摘要
目的:探讨急性胆汁淤积诱导大鼠肝细胞损伤的病理机制。方法:48只雄性SD大鼠随机分为胆总管结扎组(BDL组)和假手术组(SO组),分别于手术后1、3、5、7d取血及肝组织(n=6),检测血清胆红素、胆汁酸(BA)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(γ-GT)、丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST)水平变化。应用HE染色、Siriusred染色、电镜及DNA电泳对肝组织学、超微结构及凋亡生化学进行评估。结果:与SO组相比,BDL组大鼠血清胆红素、BA、ALP、γ-GT、ALT及AST在7d内呈进行性增高。HE及Siriusred染色显示,逐渐加重的胆管扩张伴明显的肝细胞肿胀、坏死及以胆管增生为主的结缔组织沉积。电镜显示,肝细胞内见异常包涵物,毛细胆管扩张、纤毛减少。两组大鼠肝组织均未见典型肝细胞凋亡形态及DNAladder改变。结论:急性胆汁淤积诱导的肝损伤以肝细胞坏死为主,肝细胞凋亡少见。
Objective To investigate the pathomechanism of hepatocyte injury induced by acute cholestasis in rats.Methods 48 male SD rats were randomly divided into bile duct ligation(BDL) group and sham operation(SO) group.Blood samples and hepatic tissues were collected on days 1, 3, 5 and 7.Serum levels of bilirubin(BIL), bile acid(BA), alkaline phosphatase(ALP), glutamyl transpeptidase(γ-GT), alanine aminotransferase(ALT), and aspartate aminotransferase(AST) were detected.The hepatic histopathology and ultramicrost...
出处
《实用医学杂志》
CAS
北大核心
2009年第5期700-702,共3页
The Journal of Practical Medicine
基金
江西省卫生厅基金资助项目(编号:0301104)
