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肝细胞生长因子在心肌缺血-再灌注损伤中的表达改变及意义 被引量：2

Investigation of changing and significance of HGF mRNA expression during myocardial ischemia-reperfusion process
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摘要 [目的]通过对缺血再灌注心肌细胞内肝细胞生长因子(HGF)mRNA及心肌细胞内MDA和SOD检测,揭示缺血再灌注过程中HGF mRNA表达上调的可能机制及作用。[方法]雄性SD大鼠40只,随机分为假手术组及实验组,每组20只,建立缺血再灌注损伤的模型。对比两组间心肌HGF mRNA、MDA及SOD的含量。[结果]大鼠心电图显示冠脉结扎后ST段明显抬高,打开结扎线后ST段回落,表明心肌缺血再灌注有效。与假手术组SOD(95.311±3.472)U/mL、MDA(18.842±1.166)μmol/g相比,再灌注组心肌细胞SOD活性明显降低(61.257±1.242)U/mL,P<0.01;MDA含量增高至(36.396±0.653)μmol/g,二者相比差异具有显著性意义(P<0.01);心肌HGF mRNA表达,再灌注组与假手术组分别为0.72074±0.00067和0.53668±0.00103,差异有显著性意义(P<0.01)。缺血再灌注过程中,心肌HFG mRNA变化与SOD变化成正相关,与MDA呈负相关。[结论]自由基的损伤性刺激可能是再灌注损伤过程中引起心肌HGF mRNA表达上调的原因之一。 [Objective] To test the level of HGF mRNA,SOD and MDA values within the myocardial cell during the process of ischemia reperfusion in order to investigate the possible mechanisms and function of the increased expression of HGF mRNA.[Methods] A total of 40 male SD rats were randomly divided into sham group and the experimental group,and then duplicate the model of ischemia-reperfusion injury.We compared the level of MDA,SOD and HGF mRNA values between the model group and the sham group.[Results] After the co...
作者 付饶 赵肸 金成 王艳 鲁晓宁 任焕所 姜妙娜 赵赫男
机构地区 大连医科大学 大连医科大学病理生理学教研室
出处 《大连医科大学学报》 CAS 2009年第1期42-44,74,共4页 Journal of Dalian Medical University
关键词 心肌缺血-再灌注损伤 氧自由基 肝细胞生长因子 myocardial ischemia-reperfusion injury（MIRI） oxygen free radical（OFR） hepatocyte growth factor （HGF）
分类号 R363 [医药卫生—病理学]
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共引文献10

同被引文献33

  • 1李晓东,黄跃生,张家平.机械牵张对缺血缺氧心肌细胞肌球蛋白重链mRNA表达的影响[J].中华烧伤杂志,2004,20(3):138-140. 被引量：18
  • 2王丹波,王林富,许加广,汪钊.醋蛋浸泡中蛋白质的变化及醋蛋粉的制备研究[J].食品科技,2006,31(5):48-50. 被引量：17
  • 3刘桂芝,陈祖培,王婉真,张朝,叶振坤,韦迎娜,阎玉芹.碘缺乏和碘过量对大鼠心肌肌球蛋白重链基因表达的影响[J].中国地方病学杂志,2006,25(6):618-621. 被引量：8
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大连医科大学学报
2009年 第1期

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