摘要
目的:研究聚酰胺树脂纯化贯叶金丝桃中金丝桃苷的工艺条件。方法:采用HPLC法测定金丝桃苷的含量;单因素法考察聚酰胺树脂对金丝桃苷的吸附性能以及洗脱参数。结果:聚酰胺树脂对提取液中金丝桃苷的最佳分离条件为:上柱液浓度为1.2 mg.mL-1、流速为3.6 BV.h-1,金丝桃苷比吸附量达2.33 mg.g-1,金丝桃苷纯度可达28.96%。结论:聚酰胺树脂可用于贯叶金丝桃中金丝桃苷的分离富集。
Objective:To study the technological parameters of the purification process of hyperoside from Hypericum perforatum extrative.Methods: HPLC was used to analyze the content of hyperoside in the samples from the different technological process,and the adsorption and elution parameters were investigated.Results: The optimal parameters were as follows:the sample extract solution,whose content of hyperoside was 1.2 mg·mL^(-1),was passed through the packed column with a flow rate of 3.6 BV·h^(-1).The adsorption r...
出处
《中国实验方剂学杂志》
CAS
2008年第11期9-11,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
聚酰胺树脂
贯叶金丝桃
金丝桃苷
吸附
纯化
polyamide resin
Hypericum perforatum L.
hyperoside
adsorption
purification
