摘要
从海洋真菌Phomopsis sp.分离得到的南强菌素是新发现的高抗肿瘤活性化合物,为新颖罕见的去乙酰真菌环氧二烯类化合物(Deacety Mycoepoxydiene)。以Phomopsis sp.A123经诱变后获得的818菌株为出发菌株,对其进行液体发酵培养。考察了几种常见培养基对南强菌素发酵生产的影响,确定出较适的培养基。同时以该培养基为基础培养基,探讨了碳源、氮源以及几种维生素对南强菌素积累的影响。培养条件的初步优化结果表明:最佳的培养基为PD培养基;最适碳源和氮源分别为麦芽糖及马铃薯煮汁;生物素的添加有利于南强菌素的积累,产量提高了25%。
Deacety Mycoepoxydiene is a novel antitumor agent extracted from a marine endophytic fungi Phomopsis sp.A123.In order to improve the yield of Deacety Mycoepoxydiene,the liquid fermentation medium composition is optimized by a one-factor-at-a-time method for a mutant No.818,which is derived from Phomopsis sp.A123.Potato dextrose(PD) culture medium is selected as the preliminary culture medium,and the optimal carbon and nitrogen sources for liquid fermentation are investigated.The effect of several vitamins,i...
出处
《现代化工》
CAS
CSCD
北大核心
2008年第S2期275-277,279,共4页
Modern Chemical Industry
基金
国家高技术研究发展("863")计划海洋技术领域海洋微生物产品中试研究重点项目资助
关键词
海洋真菌
抗肿瘤化合物
南强菌素
培养基优化
marine endophytic fungi
antitumor agent
Deacety Mycoepoxydiene
medium optimization
