摘要
实时定量RT-PCR广泛应用于定量检测mRAN表达水平,为基础研究、分子药物学和生物技术研究提供了一种有力的方法。该法具有易操作、高通量、敏感性高和特异性强的特点,随着新酶、新探针和新仪器的发展而得到快速的发展。本文将对实时定量RT-PCR的定量原理、仪器应用、探针种类的研究进展以及细胞因子mRNA表达水平检测上的应用作一综述。
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can combine high sensitivity with reliable specificity. The technology is evolving rapidly with the introduction of new enzymes, probes and instrumentation. In this review we discuss the principle of real-time RT-PCR, the different metho...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第S1期145-150,共6页
Immunological Journal
基金
"中国科学院百人计划"资助项目
关键词
细胞因子
定量
实时定量RT-PCR
Cytokines
Quantification
Real-time reverse transcription polymerase chain reaction
