摘要
目的研究回转模拟失重条件下 ,心肌成纤维细胞中某些生长因子表达与分泌的变化 ,以及细胞外信号调节激酶 (extracellularsignal regulatedkinase ,ERK)信号通路的活化状态。方法通过新生大鼠原代培养获取心肌成纤维细胞 ,利用水平回转器模拟失重效应 ,对碱性成纤维细胞生长因子 (bFGF)和转化生长因子β1 (TGFβ1 )的表达进行蛋白质印迹 (westernblotting)分析 ,以放射免疫检测方法测定细胞培养上清液中血管紧张素II(AngII)浓度的变化 ;利用westernblotting对ERK1 /2及磷酸化ERK1 /2分别进行检测并分析其活化状态。结果与对照组比较 ,回转后心肌成纤维细胞的bFGF表达增加 ,TGFβ1表达没有明显变化 ,AngII分泌增加 ;ERK1 /2表达总量增加 ,但其磷酸化水平下降。结论不同的生长因子对回转模拟失重有不同的响应 ;作为生长因子的重要下游信号通路 ,ERK1 /2的活化受到抑制。
Objective To study the changes of expression and secretion of some growth factors and ERK1/2 activation in cardiac fibroblasts under clinorotation conditions. Method Primary cultured neonatal rat cardiac fibroblasts were clinorotated to simulate weightlessness. The bFGF and TGFβ1 expressions in the cells and Ang II concentration in the medium were detected by western blotting and radioimmunoassay respectviely. The expression and phosphorylated level of ERK1/2 were analyzed by western blotting. Result Compared to the control group, bFGF expression and Ang II concentration increased in clinorotation group, but there was no evident change in TGFβ1 expression. At the same time, the expression of ERK1/2 increased but their phosphorylated level decreased. Conclusion Different growth factors responds to clinorotation by different ways. As one of the most important reactions of growth factor signal downstream pathways, ERK1/2 activation is inhibited in clinorotation.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2003年第z1期532-537,共6页
Space Medicine & Medical Engineering
基金
国家高技术研究发展计划 ( 863计划 )资助 ( 863 2 2 10 3 )
关键词
失重模拟
回转器
心肌成纤维细胞
生长因子
信号传导
weightlessness simulation
clinostats
cardiac fibroblasts
growth factors
signal transduction
