摘要
目的探讨丹参酸乙(SA-B)对血管紧张素Ⅱ(AngⅡ)孵育的新生大鼠心脏成纤维细胞的影响。方法将培养的心脏成纤维细胞分组加药后,采用 Alamar blue 荧光分析法和逆转录多聚酶链反应(RT-PCR)技术分别测定细胞增殖和 c-fos 基因的转录水平。结果 10μmol/L SA-B 和100μmol SA-B 可有效抑制由 AngⅡ引起的心脏成纤维细胞增殖(P<0.05)。1μmol/L SA-B 和10μmol/L SA-B 也可有效抑制 c-fos 基因 mRNA的表达(P<0.05)。结论一定浓度的丹参酸乙可抑制由 AngⅡ引起的心脏成纤维细胞的增殖和 c-fos 基因的表达水平。
Objective To study the effect of salvianolic acid B(SAB)on cardiac fibroblast of newborn rats incubated with an- giotensin Ⅱ(Ang Ⅱ).Methods Cultured cardiac fibroblasts were grouped and treated.The cell proliferation and c-fos gene transforming levels were determined by Alamar blue fluorescent analysis,and RT-PCR technique.Results SAB of 10 and 100 μmol/L concentration could effectively inhibit the proliferation of cardiac fibroblast induced by Ang Ⅱ(P< 0.05),that of 1 and 10 μmol/L also could inhibit th...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第S1期1-3,共3页
Chinese Journal of Integrated Traditional and Western Medicine
