摘要
本试验通过对健康油菜组织表面消毒、内生真菌的分离培养和鉴定 ,获得以下结果 :共获得内生真菌 4 5种 ,其中子囊菌 1种 ,半知菌 4 3种 (丝孢纲 4 0种 ,腔孢纲 3种 ) ,担子菌 1种 ,分离物中 37个种分属于 2 0个不同属 ,8个种归属待定。从分离几率来看 ,没有明显优势的种类存在 ,在叶部分出率最高的是Alternariaalternata ,分离几率仅为 7.0 4 %。不同油菜器官的内生真菌种类有一定的差异 ,从叶部分离出 2 5种 ,根部分离出 12种、花器 11种、茎 12种、幼苗 2种。以上结果显示油菜内生真菌复杂、具有丰富的种类多样性。
In this survey,we isolated endophytic fungi from surface-sterilized healthy oilseed rape( Brassica napus ) and identified them.Forty five endophytic fungi were achieved,including 1 Ascomycetces, 1 Basidiomycetes and 43 Fungi Imperfecti(40 in Hyphomycetes and 3 in Coelomycetes ).Thirty seven belonged to 20 genera,and 8 species were unidentified.There were no apparent dominant species. Alternaria alternate from leaf was the highest frequency,7.04%.The species amounts of endophytic fungi isolated from different organs of oilseed rape was variable.We found 25 species from leaves,12 species from roots,11 species from flowers,12 species from stems and 2 species from seedlings.It could be seen from our results that oilseed rape was rich in endophytic fungi.
出处
《石河子大学学报(自然科学版)》
CAS
2004年第z1期66-68,共3页
Journal of Shihezi University(Natural Science)
关键词
油菜
内生菌
多样性
Brassica napus
entophytes
diversity