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肾小管周围毛细血管损伤与肾小管上皮细胞凋亡的关系

The Relationships Between PTC Injury and Renal Tubular Cell Apoptosis Induced by Complete Ureteral Obstruction
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摘要 目的观察单侧输尿管完全梗阻(CUUO)模型中肾小管周围毛细血管(PTC)损伤与肾小管上皮细胞凋亡的关系。方法建立SD大鼠CUUO模型,用免疫组化方法检测PTC的密度,TUNEL法对PTC和肾小管上皮细胞进行原位凋亡测定,透射电镜显示超微结构变化。结果梗阻第1周,Flk-1阳性PTC数量变化不显著,肾小管上皮细胞凋亡很少见。第2、4周,Flk-1阳性的PTC数量减少,肾小管扩张或萎缩明显。电镜显示肾小管上皮细胞、PTC内皮细胞的死亡形式主要为凋亡;TUNEL显示肾小管上皮细胞凋亡在第14 d达到高峰,然后迅速下降。在梗阻第2周时,PTC密度与肾小管上皮细胞凋亡负相关(r=-0.863,P<0.01)。结论PTC损伤是以内皮细胞凋亡为主要特征,PTC减少在肾小管上皮细胞凋亡的过程中发挥了重要作用。 Objective To study the relationships between PTC injury and renal tubular cell apoptosis induced by complete ureteral obstruction.Methods Complete unilateral ureteral obstruction was induced in SD rats by ligation of the left ureter.PTC density and Flk-1 were immunohistochemically detected,and morphologic changes in PTC endothelial cells and renal tubular epithetial cells were examined by using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL) technique and electron-microsc...
作者 樊彩斌 温端改 侯建全 严春寅 周云 严向明 王兴东
机构地区 苏州大学附属第一医院泌尿外科 苏州大学附属儿童医院泌尿外科
出处 《苏州大学学报（医学版）》 CAS 北大核心 2008年第1期59-61,175,共4页 Suzhou University Journal of Medical Science
关键词 单侧输尿管完全梗阻 肾小管周围毛细血管 凋亡 complete unilateral ureteral obstruction peritubular capillary apoptosis
分类号 R692.6 [医药卫生—泌尿科学]
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参考文献8

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苏州大学学报（医学版）
2008年 第1期

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