摘要
目的:观察As2O3对血管内皮细胞增殖、凋亡和细胞周期的干扰,探讨As2O3对血管内皮细胞生长的直接影响。方法:血管内皮细胞EVC-304体外培养,As2O3干预。采用MTT测定细胞活性,流式细胞仪测定细胞周期、细胞凋亡。结果:ECV-304细胞经过As2O3干预,其存活率明显降低,并且呈剂量依赖性。As2O3干预后,进入S期的细胞减少,细胞周期被阻在G1期,细胞可能在G1期进入凋亡。As2O3诱导早期凋亡是对照组的2.88～5.1倍,并且呈剂量依赖性;其晚期凋亡是对照组的1.17～1.67倍。结论:As2O3可直接干扰血管内皮细胞的细胞周期,阻滞细胞在G1期,并诱导细胞凋亡,进而抑制血管内皮细胞的增殖。
Objective:To study the effecs on effect of arsenic trioxide on proliferation and cell cycle of vascular endothelial cells.Methods:ECV304 cells were treated with As2O3.Cell viability of ECV304 cells was measured by MTT assay.Cell cycle was analyzed by using flow cytometry with PI staining;apoptosis was estimated by flow cytometry by Annexin V-FITC conjugated with PI staining.Results:FCM assays showed that the percent of the cells treated with As2O3 were higher in sub-G1,lower in sub-S and lower in G2/G1 than...
出处
《陕西医学杂志》
CAS
北大核心
2008年第6期651-653,共3页
Shaanxi Medical Journal
基金
陕西省自然科学基金项目(2002C239)
西安交通大学医学院第二附属医院科研基金(2003-YL-35)
关键词
三氧化二砷
细胞增殖
细胞周期
血管内皮细胞
Arsenic trioxide Proliferation Cell cycle Vascular endothelial cells
