摘要
以不同抗性品种柱花草为材料,研究接种胶胞炭疽菌后过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及H_2O_2、胼胝质含量的变化。结果表明:与对照相比,除高感品种的CAT活性于接种后降低外,高抗品种及中抗品种在试验中均有升高过程,其最大增幅与品种的抗病性呈正相关。除高抗品种的SOD活性于接种后降低外,其他品种在试验中均有升高过程,高感品种的增幅均大于中抗品种,且出现增幅的时间早于中抗品种,SOD活性的增幅与品种的抗病性呈负相关。接种后24 h,高抗与中抗品种接种处理的H_2O_2含量均升至最大值,而高感品种H_2O_2含量达最大值的时间稍滞后,高抗品种能更快、有效地调动H_2O_2介导的防御机制。高抗品种胼胝质含量大约是其他品种的3~5倍,且与抗病性有关,中抗品种TPRC90087接种处理与对照的胼胝质含量差异较小,中抗品种CIAT184和高感品种在受病原菌侵染后可能通过胼胝质的积累来抵御病害。
The activities of catalase(CAT),superoxide dismutase(SOD),the contents of hydrogen peroxide and callose were measured in four cultivars of Stylosanthes differing in disease resistance,Tardio,CIAT184,TPRC90087 and Graham, following inoculation with conidial suspension of Colletotrichum gloeos poriodes.CAT activity was induced and increased in the high or mediately resistant cultivars during at experimental period except the susceptible cultivar after the inoculation.It seemed that CAT activity was positive c...
出处
《扬州大学学报(农业与生命科学版)》
CAS
CSCD
北大核心
2007年第2期73-77,94,共6页
Journal of Yangzhou University:Agricultural and Life Science Edition
基金
广东省自然科学基金重点资助项目(04105978)
关键词
柱花草
胶胞炭疽菌
防御酶
过氧化氢
胼胝质
Stylosanthes spp
Colletotrichum gloeosporiodes
defense enzymes
hydrogen peroxide
callose