摘要
本文利用高产β-葡萄糖苷酶菌种制备游离细胞和固定化细胞,在温和条件下可将京尼平苷转化为京尼平,转化率高达98%。通过条件优化,得到了最优转化条件:京尼平添加量1.5%,pH自然,摇瓶装量20%,28℃、150rpm转化24h。固定化细胞可连续使用4次。这种微生物转化法安全、高效,产品纯度高,是生产京尼平的一种新方法。
This new method is used to produce free cells and immobilized cells by the fungus with high β-glucosidase activity.It can transform geniposide into genipin under mild conditions and the rate of transformation could reach 98%.The optimum conditions of fermentation were adding 20% medium containing 1.5% geniposide into erlenmeyer flask and then incubating at 28 ℃ with shaking at the speed of 150 rpm for 24 h.Immobilized cells can be used no more than 4 times.This microbial transformation method is a security ...
出处
《天然产物研究与开发》
CAS
CSCD
2008年第1期70-73,共4页
Natural Product Research and Development
关键词
京尼平
京尼平苷
固定化细胞
微生物转化
geniposide
genipin
immobilized cells
microbial transformation
