摘要
目的探讨异质性细胞核核糖蛋白A2B1(HNRNPA2B1)在前列腺癌中的作用,并进一步筛选靶向治疗前列腺癌的中药活性成分。方法通过GEPIA、UALCAN和THPA数据库分析HNRNPA2B1在前列腺癌组织及正常组织中的表达水平及与前列腺癌预后关系,并分析HNRNPA2B1表达水平与前列腺癌患者临床特征关系。使用TIMER数据库分析HNRNPA2B1与前列腺癌免疫细胞浸润的相关性。使用LinkedOmics和DAVID数据库分析HNRNPA2B1基因的正相关基因和KEGG通路。使用比较毒理基因组数据库(CTD)筛选能靶向作用于HNRNPA2B1的中药活性成分。结果HNRNPA2B1在前列腺癌中高表达,与前列腺癌预后相关,且与淋巴结转移及Gleason评分有关。HNRNPA2B1与细胞纯度、B细胞、CD8+T细胞、巨噬细胞、中性粒细胞、树突状细胞均呈正相关(r=0.197、0.355、0.363、0.114、0.327、0.258,均P<0.05),其参与前列腺癌细胞的免疫浸润。KEGG通路分析HNRNPA2B1及其正相关基因主要参与剪切体通路和泛素介导的蛋白水解通路。进一步通过比较毒理基因组数据库筛选发现槲皮素、白藜芦醇、染料木黄酮等可能是靶向作用于HNRNPA2B1的中药活性成分。结论HNRNPA2B1在前列腺癌中高表达,与前列腺癌预后及免疫浸润有关。槲皮素、白藜芦醇、染料木黄酮等中药活性成分可能通过靶向作用于HNRNPA2B1在前列腺癌中发挥作用。
Objective To investigate the role of heterogeneous nuclear ribonucleoprotein A2B1(HNRNPA2B1)in prostate cancer,and to screen the active components of traditional Chinese medicine for targeted treatment of prostate cancer.Methods The GEPIA,UALCAN,and THPA databases were used to analyze the expression of HNRNPA2B1 in prostate cancer tissues and normal tissues as well as its relationship with the prognosis of prostate cancer.The relationship between HNRNPA2B1 expression and clinical characteristics of prostate cancer patients was also analyzed.The TIMER database was used to analyze the correlation between HNRNPA2B1 expression and immune cell infiltration in prostate cancer.Positively related genes and KEGG pathways of HNRNPA2B1 were analyzed using the LinkedOmics and DAVID databases.The Comparative Toxicogenomics Database(CTD)was used to screen the active components of traditional Chinese medicine that target HNRNPA2B1.Results HNRNPA2B1 is highly expressed in prostate cancer,which is related to the prognosis of prostate cancer,lymph node metastasis,and Gleason score.HNRNPA2B1 participates in the immune infiltration of prostate cancer cells.KEGG pathway analysis showed that HNRNPA2B1 and its positively related genes are mainly involved in the spliceosome pathway and the ubiquitin-mediated proteolysis pathway.By searching the CTD,it was found that quercetin,resveratrol,and genistein may be the active components of traditional Chinese medicine targeting HNRNPA2B1.Conclusion HNRNPA2B1 is highly expressed in prostate cancer,which is related to the prognosis and immune invasion of prostate cancer.Quercetin,resveratrol,genistein,and other active components of traditional Chinese medicine may play a role in prostate cancer by targeting HNRNPA2B1.
作者
王苏贵
皇立媛
姜福金
吴自余
张先云
李强
严大理
Wang Sugui;Huang Liyuan;Jiang Fujin;Wu Ziyu;Zhang Xianyun;Li Qiang;Yan Dali(Department of Urology,Huai'an Hospital Affiliated to Xuzhou Medical University,Huai'an 223002,China;Department of Traditional Chinese Medicine Oncology,Huai'an Hospital Affiliated to Xuzhou Medical University,Huai'an 223002,China)
出处
《中华临床医师杂志（电子版）》
CAS
北大核心
2023年第6期731-736,共6页
Chinese Journal of Clinicians(Electronic Edition)
基金
江苏省第十六批“六大人才高峰”项目(2019-WSW-218)
淮安市自然科学基金项目(HAB201730,HAB202117)
徐州医科大学附属医院发展基金项目(XYFY202243)
