摘要
目的:探究黄芪多糖抑制内质网应激进而缓解大鼠脑缺血再灌注损伤的的作用与机制。方法:大鼠随机分为空白对照组、假手术组、模型组和黄芪多糖给药组,每组15只,预防性给药14天后阻断大脑中动脉,缺血3h后,将线栓拔出,恢复大脑血液正常供应24 h后处死大鼠,取大鼠脑组织备用。假手术组将线栓送入颈内动脉后迅速撤回。结果:模型组大鼠脑组织梗死面积比达到25.69%,而黄芪多糖预防给药组能够有效地降低大鼠脑组织缺血再灌注模型中的脑梗死面积比。缺血再灌注导致SOD含量显著下降,MDA的含量显著上调,黄芪多糖可显著上调SOD同时抑制MDA产生。与此同时黄芪多糖能够有效抑制炎症因子IL-1β、TNF-α过度产生;内质网应激相关蛋白CHOP和GRP78及促凋亡相关蛋白Bax表达显著升高,而抗凋亡相关蛋白Bcl-2蛋白水平显著抑制,黄芪多糖能够抑制内质网应激蛋白GRP78、CHOP蛋白的高表达,同时维持促凋亡和抗凋亡蛋白Bax/Bcl-2的平衡。结论:黄芪多糖通过抑制内质网应激缓解大鼠脑组织缺血再灌注损伤,并进一步抑制内质网应激引起的细胞凋亡。
Objective:To investigate the mechanism underlying the effect of astragalus polysaccharides in alleviating cerebral ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress(ERS)in rats.Methods:Experimental rats were randomized into the blank control group,sham operation group,model group and astragalus polysaccharide(AP)-medicated group(n=15 each).After the 14-day prophylactic AP medication,the middle cerebral artery was occluded.The suture thrombosis was removed after 3 h ischemia to resume normal blood supply to the brain,then the rats were sacrificed 24 h later,and harvested of brain tissues for subsequent examinations.In the sham operation group,the suture thrombois was withdrawn immediately after being advanced into the internal carotid artery.Results:The infarction area accounted for 25.69%of brain tissue in the model group.Prophylactic medication with AP could effectively reduce the percentage area of cerebral infarction in rat model of brain ischemia-reperfusion.Ischemia-reperfusion leads to a significant reduction in SOD and increase in MDA contents.Astragalus polysaccharides significantly increased SOD while inhibiting MDA production.Meanwhile,astragalus polysaccharide effectively inhibited the overproduction of inflammatory factors IL-1βand TNF-α.Ischemia-reperfusion resulted in increased expression of ERS-related CHOP and GRP78 proteins and pro-apoptotic Bax protein,and significantly inhibition of anti-apoptotic Bcl-2 protein expression.Astragalus polysaccharide could inhibit high-level expression of ERS-related GRP78 and CHOP proteins while maintaining the balance of pro-and anti-apoptotic proteins Bax/Bcl-2.Conclusion:Astragalus polysaccharide alleviates ischemia-reperfusion injury in rat brain tissue by inhibiting endoplasmic reticulum stress,which further suppresses the cell apoptosis caused by endoplasmic reticulum stress.
作者
赵硕
杨刚华
王晓
李子峰
陈强
Zhao Shuo;Yang Ganghua;Wang Xiao;Li Zifeng;Chen Qiang(Surgical Intensive Care Unit,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710000,Shaanxi,China;Department of Geriatrics,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710000,Shaanxi,China;Department of General Surgery,Zhenping County Hospital,Ankang 725000,Shaanxi,China;Department of Urology,First Affiliated Hospital of Xi’an Medical College,Xi’an 710000,Shaanxi,China;Department of Cardiovascular Surgery,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710000,Shaanxi,China)
出处
《中华生物医学工程杂志》
CAS
2020年第4期309-313,共5页
Chinese Journal of Biomedical Engineering
关键词
黄芪多糖
内质网应激
缺血再灌注损伤
细胞凋亡
Astragalus polysaccharide
Endoplasmic reticulum stress
Ischemia-reperfusion injury
Apoptosis
