摘要
蚊媒传病严重危害全世界人类的生命健康,其中,伊蚊在传播疾病方面扮演着十分重要的角色,是登革热、寨卡病毒病、黄热病、基孔肯雅病的媒介昆虫。因此,对伊蚊的控制在防治上述传染病中起着重要的作用。本项研究构建了伊蚊V-ATPA基因的RNAi表达载体pMaa7IR/V-ATPAIR,通过玻璃珠法转化莱茵衣藻(Chlamydomonas reinharditii),得到的转基因藻株喂饲伊蚊幼虫,结果显示pMaa7IR/V-ATPAIR转基因藻株对伊蚊幼虫的成活率、化蛹时间和数量、成虫羽化时间和数量均存在较大的影响。V-ATPA RNAi转基因藻株对伊蚊有一定的致死作用。由于微藻是蚊子幼虫天然食物,在自然界广泛存在,并且目前小球藻,螺旋藻,衣藻等绿藻门微藻均可进行工厂化大规模生产,技术成熟,生产成本低廉,所以可以长期大规模投放在封闭区域,形成优势藻种,这为生物杀蚊阻断登革热、寨卡病毒病等恶性传染病的流行提供新的思路。
Mosquito-borne diseases seriously endanger human life and health all over the world.Among them,the aedes mosquito plays a very important role in the transmission of diseases.It is a vector insect of Dengue fever,Zika virus disease,yellow fever,and Chikungunya disease.Therefore,the control of aedes mosquito plays an important role in preventing and controlling the above-mentioned infectious diseases.In this study,an RNAi expression vector pMaa7 IR/V-ATPAIR of the aedes mosquito V-ATPA gene was constructed,and the Chlamydomonas reinhardtii was transformed by the glass bead method.The obtained transgenic algae strain was fed to aedes larvae.The survival rate of aedes larvae,the time and number of pupations,and the time and number of adult emergences all had a great impact.The V-ATPA RNAi transgenic algae strain has a certain lethal effect on aedes mosquito.Because microalgae are natural food for mosquito larvae,they are widespread in nature.At present,chlorella,spirulina,Chlamydomonas,and other green algae microalgae can be produced on a large scale in the factory.The technology is mature and the production cost is low.It can be put into closed areas on a large scale for a long time to form dominant algae species.It provides a new idea for biological mosquito killing to prevent the spread of Dengue fever,Zika virus disease and other malignant infectious diseases.
作者
费小雯
肖洒
贺长皓
李亚军
黄晓晴
李江月
邓晓东
Fei Xiaowen;Xiao Sa;He Changhao;Li Yajun;Huang Xiaoqing;Li Jiangyue;Deng Xiaodong(Department of Biochemistry and Molecular Biology,Hainan Medical College,Haikou,571101;Hainan Key Laboratory of Functional Components and Utilization of Marine Biological Resources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou,571101)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第5期1961-1969,共9页
Genomics and Applied Biology
基金
海南省重点科技计划项目(ZDYF2018124)
国家自然科学基金项目(31870344)
农业农村部财政专项(NFZX2018)
中国热带农业科学院基本科研业务费专项资金(NO.19CXTD-32)共同资助
