摘要
表面活性素和伊枯草菌素是一类在生物防治方面具有重要作用的蛋白质。本研究利用生物信息学方法对枯草芽孢杆菌中表面活性素和伊枯草菌素的基因及蛋白序列进行了详细的分析。结果表明:其表面活性素和伊枯草菌素大多数蛋白为酸性蛋白质和不稳定蛋白,且全部为非分泌性蛋白,多数定位在细胞质中,多数成员在丝氨酸处最可能通过相应位点的磷酸化来实现其功能的调控,其二级结构主要由α螺旋、无规则卷曲、延伸链和β转角组成。表面活性素蛋白的氨基酸总数在71~393个,pI范围在4.67~9.98,跨膜域预测显示只有1个成员存在跨膜现象,亲疏水性预测显示多数为亲水蛋白,根据构建的系统发育树的分支可以将其分为8组,其中Group I成员最多,有16个,约占总数的30.2%,Group V进化速度最慢,是进化过程中保留下来的较原始的一类,而Group I分化速度最快,亲缘关系相对较远;伊枯草菌素蛋白的氨基酸总数在79~277个,pI范围在4.73~10.11,跨膜域预测显示有3个成员存在跨膜现象,亲疏水性预测显示全部为亲水蛋白,根据构建的系统发育树的分支可以将其分为6组,其中Group VI成员最多,有11个,约占总数的33.3%,Group IV进化速度最慢,是进化过程中保留下来的较原始的一类,而Group I分化速度最快,因而亲缘关系相对较远。这些结果可为今后深入研究枯草芽孢杆菌表面活性素和伊枯草菌素的生物学功能和调控机制提供一定的理论依据,为枯草芽孢杆菌生产表面活性素和伊枯草菌素提供参考。
Surfactin and iturin are proteins that play an important role in biological control. Gene and protein sequences of the surfactin and iturin in Bacillus subtilis were analyzed in detail using bioinformatics method in this study. The results showed that most proteins of surfactin and iturin in Bacillus subtilis were acid and unstable protein. All of them were non-secretive proteins. Most of them were located in the cytoplasm. Most members were likely to regulate their functions by phosphorylation of corresponding sites at serine sites. Its secondary structure was mainly composed of alpha helix, random coil, extended strand and beta turn. The total number of amino acids of surfactin protein ranged from 71 aa to 393 aa, and the pI ranged from 4.67 to 9.98. Transmembrane prediction showed that only one member had transmembrane phenomena, and hydrophilic prediction showed that most of them were hydrophilic proteins. They can be divided into 8 groups according to the branches of the phylogenetic tree. Among them, there are at most 16 members of Group I, accounting for 30.2% of the total. Group V had the slowest evolutionary speed and retained the primitive group in the evolutionary process, while Group I had the fastest differentiation speed and relatively distant genetic relationship. The total number of amino acids in iturin proteins ranged from 79 aa to 277 aa, and the pI ranged from 4.73 to 10.11. The transmembrane prediction showed that three members have transmembrane phenomena, and the hydrophilic prediction showed that all of them were hydrophilic proteins. According to the branches of phylogenetic tree, they can be divided into six groups. Group VI has 11 members, accounting for 33.3% of the total. Group IV has the slowest evolution speed and retains the primitive group in the evolutionary process. Group I differentiated the fastest and had a relatively distant relationship.These results can provide theoretical basis for further studies on biological functions and regulatory mechanisms of surfactin and iturin in Bacillus subtilis, and provide reference for production of surfactin and Ictilisin by Bacillus subtilis.
作者
易志飞
付春
江纳
杨瑶君
肖开兴
Yi Zhifei;Fu Chun;Jiang Na;Yang Yaojun;Xiao Kaixing(Key Laboratory of Bamboo Diseases and Insect Pests Control and Resource Development in Sichuan Province,College of Life Science,Leshan Normal University,Leshan,614000;College of Mathematics and Information Science,Leshan Normal University,Leshan,614000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第5期2177-2200,共24页
Genomics and Applied Biology
基金
四川省科技厅项目(801800572)
乐山师范学院人才启动项目(801800676)
乐山师范学院校级项目(80141-9001)共同资助
