摘要
目的:分别用化学发光法和胶体金法检测新型冠状病毒(2019-nCoV)特异性抗体,比较两种方法抗体检出率的差异,为新型冠状病毒肺炎(COVID-19)的诊疗提供参考。方法:纳入2020年2月16日至2月29日就诊于武汉雷神山医院确诊和疑似COVID-19患者,共191例,根据入院及抗体测定时核酸检测结果分为2019-nCoV持续阳性组、2019-nCoV阳转阴组及2019-nCoV疑似组。并另外收取27例排除COVID-19患者作为对照组,用以检测测量方法的特异性。采集血清样本,分别用胶体金法和化学发光法检测各组IgM+IgG总抗体及IgM抗体。使用SPSS 24.0比较两种方法抗体检出率差异。结果:胶体金法和化学发光法检测2019-nCoV特异性抗体临床特异性为100%。持续阳性组胶体金法和化学发光法无明显差异。阳转阴组和疑似组化学发光法检测总抗体的阳性检出率明显优于胶体金法(95.2%vs 76.2%,94.9%vs 70.5%),差异有统计学意义(P<0.05)。化学发光法检测IgM在3组间无明显差异。结论:在COVID-19的诊疗过程中,抗体的检测可以辅助弥补咽拭子核酸检测的不足,化学发光法测定总抗体优于胶体金法,化学发光法检测IgM适用于疾病各个阶段。
Objective:To compare the chemiluminescence and colloidal gold method in the detection of2019 novel Coronavirus(2019-nCoV,or SARS-CoV-2)specific antibody and to evaluate the importance in the diagnosis and treatment of COVID-19.Methods:A total of 191 cases of diagnosed and suspected COVID-19 patients admitted in Febrary 2020 in Wuhan Leishenshan Hospital were included.The patients were divided into 2019-nCoV continuous positive group,2019-nCoV positive to negative group,and 2019-nCoV suspect group according to the results of pharyngeal swab 2019-nCoV nucleic acid detection.In addition,27 patients without COVID-19 were set as control group to test the specificity.The serum samples were collected,and colloidal gold and chemiluminescence method were used to detect total antibodies(IgM+IgG)and IgM.SPSS 24.0 was used to compare the difference in antibody detection rates between the two methods.Results:The specificity of total antibodies(IgM+IgG)and IgM detection was 100%.Two methods of total antibody detection had no significant difference in continuous positive group(P=1.00).In positive to negative group and suspect group,the positive detection rate of chemiluminescence method was significantly higher than colloidal gold method(95.2%vs 76.2%and 94.9%vs 70.5%,respectively,both P<0.05).The chemiluminescence method showed no significant difference between the three groups in IgM detection(P>0.05).Conclusion:The detection of 2019-nCoV specific antibody can be an important supplemental method to pharyngeal swab 2019-nCoV nucleic acid detection during the diagnosis and treatment of COVID-19.The chemiluminescence method is better than colloidal gold method in the detection of total antibody.The IgM detection using chemiluminescence method is suitable for all stages of the disease.
作者
唐鹏
赵自武
刘颖娟
陈雅文
彭定辉
王冬
李一荣
谢文
TANG Peng;ZHAO Ziwu;LIU Yinjuan;CHEN Yawen;PENG Dinghui;WANG Dong;LI Yirong;XIE Wen(Dept.of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei,China)
出处
《武汉大学学报（医学版）》
CAS
2020年第4期517-520,共4页
Medical Journal of Wuhan University
