摘要
氮源是生物过滤塔高效稳定净化甲烷(CH^(4))废气的关键因素,然而关于畜禽养殖废水中铵氮是否可以作为除CH^(4)生物过滤塔氮源以及相应的作用机制尚不明晰。启动并成功运行2个生物过滤塔BF_no(对照,无氮源)和BF_A(铵态氮为氮源),比较分析了不同停留时间(EBRT)下,2个生物过滤塔的CH^(4)净化性能,并采用宏基因组技术解析了铵态氮影响生物过滤塔CH^(4)净化性能的微生物学机理。结果表明,生物过滤塔BF_A的CH^(4)去除性能优于生物过滤塔BF_no,当EBRT为44 min时,BF_A的CH^(4)去除效率稳定在80%以上,而生物过滤塔BF_no的CH^(4)去除效率不足70%。宏基因组分析结果表明:BF_no和BF_A具有显著不同的微生物群落结构,其中硝化螺旋菌门(Nitrospirae)是生物过滤塔BF_A中的特有菌属。生物过滤塔BF_A中与硝化过程相关的amoA和hao基因,以及与CH^(4)氧化相关的fae、mtdA和fmdA基因相对丰度均显著高于BF_no(P≤0.05),证明以铵态氮为氮源的生物过滤塔BF_A中不仅具有较高的硝化能力,还具有较高的CH^(4)氧化能力。本研究结果可为生物过滤法净化畜禽养殖含CH^(4)废气和液态养殖粪污的综合利用提供参考。
Nitrogen is the key factor for the efficient and stable operation of biofilters treating methane(CH^(4))containing gases.However,it is unclear whether the livestock wastewater with high concentrations of NH4+-N can be used as the nitrogen source of biofilter for CH^(4)removal and the corresponding mechanism.This study successfully operated two biofilters treating simulated CH^(4)containing gases,BF_no(control,no nitrogen source)and BF_A(NH4+-N as nitrogen source),and compared CH^(4)purification performances of the two biofilters under different empty bed residence time(EBRT).The corresponding microbiological mechanism was investigated based on metagenomic analysis.The results showed that BF_A exhibited a better CH^(4)purification performance in comparison with BF_no.When the EBRT was 44 min,the CH^(4)removal efficiency of BF_A was stable at more than 80%,while that of BF_no was less than 70%.The two biofliters had significantly different microbial community structures.The species Nitrospirae showed a quite high relative abundance in BF_A.The relative abundance of amoA and hao genes related to nitrification process,and the relative abundance of fae,mtdA and fmdA genes related to CH^(4)oxidation in BF_A were significantly higher than that in BF_no(P≤0.05),which proved that BF_A with NH4+-N as nitrogen source had higher nitrification and CH^(4)oxidation ability.
作者
刘臻岳
范玉婧
陈苗苗
姚惠娇
高志岭
刘春敬
谢建治
耿仕呈
代宇菲
LIU Zhenyue;FAN Yujing;CHEN Miaomiao;YAO Huijiao;GAO Zhiling;LIU Chunjing;XIE Jianzhi;GENG Shicheng;DAI Yufei(College of Resources and Environmental Sciences/Key Laboratory of Farmland Ecological Environment of Hebei Province,Baoding071000,China;Hebei Animal Husbandry Station,Shijiazhuang050000,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2023年第3期1001-1010,共10页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金资助项目(51908189)
河北省自然科学基金资助项目(B2022204029)
河北省在读研究生创新能力培养资助项目(CXZZBS2023072)
