摘要
以探究污水处理系统反硝化速率与活性污泥反硝化功能基因活性之间的联系为目的,建立反硝化反应器装置,以实际水质净化厂缺氧池污泥作为研究对象,考察了其微生物菌群结构和反硝化效能并进行了分子生物学分析。结果表明:缺氧池活性污泥中存在硝化螺旋菌科(Nitrospiraceae)和红环菌科(Rhodocyclaceae)两种与氮代谢相关的优势菌群。反应器模拟废水进水NO^(-)_(3)-N浓度为30 mg·L^(-1),经过反应器运行约2 h,可去除约95%的NO^(-)_(3)-N,平均反硝化速率为3.31 mg·(g·h)^(-1);瞬时反硝化速率呈现不规则变化,最高瞬时反硝化速率为6.47 mg·(g·h)^(-1);经分子生物学qPCR实验测定,活性污泥反硝化功能基因活性(包括narG、nirK、nirS、norB和nosZ)也呈现不规则变化;通过对数据相关性、拟合度分析,证明反硝化速率与反硝化功能基因活性存在一定程度上的相关性,其中关联程度最高的是编码亚硝酸盐还原酶的nir基因。
In order to explore the relationship between the denitrification rate of sewage treatment system and the activity of sludge denitrification functional genes,the denitrification reactor equipment was built.Taking sludge from anoxic tanks of sewage treatment plants as research objects,investigate its microbial flora structure and denitrification efficiency,and conduct molecular biology analysis.The results showed that Nitrospiraceae and Rhodocyclaceae exist in the sludge related to nitrogen metabolism.The concentration of NO^(-)_(3)-N in the simulated wastewater of the reactor is 30 mg·L^(-1),the reactor runs for about 2 hours and can remove about 95%of NO^(-)_(3)-N.the average denitrification rate is 3.3109 mg·(g·h)^(-1),the instantaneous denitrification rate presents irregular changes,which is up to 6.4748 mg·(g·h)^(-1).Determined by molecular biology qPCR experiment,the activity of sludge denitrification functional genes also showed irregular changes,including narG、nirK、nirS、norB and nosZ.Through the analysis of the data correlation and the degree of R-squared,it is proved that to a certain extent,the denitrification rate and the activity of denitrification functional genes are highly correlated,and the nir gene which codes for nitrite reductase is the most correlated.This research helps sewage treatment plant quickly and accurately detect the sludge denitrification activity,provides convenient and fast detection methods for energy efficiency monitoring,ensuring the normal operations of sewage treatment enterprises.
作者
周星煜
张金松
ZHOU Xingyu;ZHANG Jinsong(Shenzhen Water(Group)Ecological&Environmental Technology Co.,Ltd.,Shenzhen 518031,China;School of Environment,Harbin Institute of Technology,Harbin 150090,China;Shenzhen Water and Environment Group Co.,Ltd.,Shenzhen 518031,China)
出处
《给水排水》
CSCD
北大核心
2022年第S01期711-717,共7页
Water & Wastewater Engineering
关键词
反硝化功能基因
脱氮效率
关联性
检测手段
Denitrification functional genes
Nitrogen removal efficiency
Relevance
Detection means
