摘要
为了探索海上石油终端生产污水A/O生化处理中的菌群构效关系,使用氯气校正法测量COD含量和基于16S r DNA基因构建克隆文库。结果发现上岸油水样OW、厌氧处理ABR进口、ABR出口和好氧处理SBR出口污水的COD分别为(630±52)、(776±40)、(385±31)、(76±13)mg/L。文库的α多样性指数表明ABR的微生物种类最多;冗余分析表明COD含量对SBR菌群影响最小。对于微生物组成,OW主要包含:Tepidiphilus(33.6%)、脱硫弧菌(9.0%)、脱铁杆菌(7.40%)和紫单胞菌(4.9%);ABR:海细菌(15.6%)、弓形杆菌(9.4%)、互营菌(7.0%)、Desulfocaldus(4.7%)和脱硫微菌(2.3%);SBR:Caloranaerobacter(20.2%)、着色菌(8.3%)和小红卵菌(2.4%)。这些发现有助于提高海上油田生产污水处理效率、减少海洋石油工业对海洋环境的影响。
To explore the microbial communities and structure activity relationship in the waste water treatment by A/O process on an offshore oil and gas terminal,COD was determined by chlorine correction method and 16 S rDNA gene clone libraries were constructed.The COD of the water samples onshore oilwater OW,the influent of anaerobic ABR,the effluent of ABR and the effluent of aerobic SBR were 630±52,776±40,385±31,76±13 mg/L,respectively.Theαdiversity indexes showed that ABR was most abundant in microbial species;and redundancy analysis demonstrated that the effect of COD on microbial community of SBR was minimum.Microbial composition of OW:Tepidiphilus(33.6%),Desulfovibrio(9.0%),Deferribacteraceae(7.40%)and Porphyromonadaceae(4.9%);ABR:Marinobacterium(15.6%),Arcobacter(9.4%),Syntrophomonas(7.0%),Desulfocaldus(4.7%)and Desulfomicrobium(2.3%);and SBR:Caloranaerobacter(20.2%),Chromatiales(8.3%)and Rhodovulum(2.4%).These findings could be used for improving the efficiency of production water treatment and alleviating the potential pollution caused by offshore oil and gas industry.
作者
苏三宝
王林海
林军
喻高明
佘跃惠
Su Sanbao;Wang Linhai;Lin Jun;Yu Gaoming;She Yuehui(School of Petroleum Engineering,Yangtze University,WuHan 430100,China;CNOOC EnerTech-Drilling&Production Co.,ZhanJiang Guangdong 524057,China)
出处
《科技通报》
2020年第3期124-129,共6页
Bulletin of Science and Technology
基金
国家自然科学基金(51474034和51574038)
关键词
海上石油终端
化学需氧量
微生物多样性
冗余分析
构效关系
offshore oil and gas terminal
microbial community
chemical oxygen demand(COD)
redundancy analysis
structure activity relationship
