摘要
莱克多巴胺俗称“瘦肉精”,可被用来饲养牲畜以提高胴体瘦肉率。食用含有莱克多巴胺的畜禽肉或内脏可引起健康问题甚至危害生命。传统的莱克多巴胺检测周期较长,费时费力,不利于实际大范围推广使用。在一些屠宰场往往采用抽检的方式进行检测,存在严重滞后性。研发了一种猪肉中“瘦肉精”莱克多巴胺便携式快速筛查装置,该装置主要包括光谱采集模块、光源模块、控制模块和电源。并基于NI LabVIEW软件开发工具,采用G语言编写猪肉“瘦肉精”智能快速检测的控制软件。首先,在碱性环境下利用乙酸乙酯对猪肉中莱克多巴胺进行提取,并采用表面增强拉曼散射(SERS)方法进行检测。研究对比了不同浓度NaCl水溶液作为聚集剂对莱克多巴胺SERS光谱的影响,结果表明以1 mol/L NaCl为聚集剂的增强效果最好。其次,比较了液滴蒸发对拉曼信号的影响,结果表明在滴加样品后,4 s后采集的拉曼信号较好。然后,制备不同莱克多巴胺含量(1、2、4、6、8、10μg/g)的猪肉样品进行定量分析,采用自动惠塔克拟合算法(AWF)对光谱数据进行预处理,扣除原始拉曼光谱中包含的荧光背景。建立836 cm^(-1)处SERS强度与猪肉样品中莱克多巴胺含量之间的一元线性回归模型。结果表明,模型具有较好的线性关系,决定系数R^(2)为0.99,均方根误差为0.178μg/g。最后,重新制作一批莱克多巴胺含量相同的猪肉样本,利用研发的装置对猪肉中莱克多巴胺进行检测,预测值与样品标准理化值具有较好的线性关系,决定系数R^(2)为0.99,均方根误差为0.317μg/g。本装置简单便携,价格便宜,检测时间小于1 h,检出限为1μg/g,可以用于猪肉中莱克多巴胺的快速筛查。
Ractopamine is a syntheticβ-receptor agonist that can be used to feed livestock to improve lean meat yield.Ractopamine can cause health problems and even endanger life if eaten from livestock,poultry or viscera.Traditional ractopamine detection cycle is long,time-consuming and laborious,which is not conducive to the actual large-scale promotion of use.In some slaughterhouses,sampling inspection is often used for testing which is a serious lag.A portable rapid screening device for ractopamine in pork was developed.The device mainly included spectra acquisition module,light source module,control module,power supply and computer.Based on NI LabVIEW software development tool,G language was used to write the control software of intelligent and rapid detection of pork“clenbuterol”.Firstly,ractopamine was extracted from pork by ethyl acetate in alkaline environment,and detected by SERS method.The effects of several different concentrations of NaCl aqueous solutions as aggregators on SERS spectra of ractopamine were compared.The results showed that 1 mol/L NaCl as aggregator had the best enhancement effect.Secondly,the effect of droplet evaporation on Raman signal was compared.The results showed that the Raman signal was better after 4 s.Then,pork samples with different ractopamine contents(1μg/g,2μg/g,4μg/g,6μg/g,8μg/g and 10μg/g)were prepared for quantitative analysis.Automatic Whittaker fitting algorithm(AWF)was used for preprocessing,and the fluorescence background contained in the original Raman spectrum was deducted.A linear regression model was established between SERS intensity at 836 cm^(-1)and ractopamine content in pork samples.The results showed that the model has a good linear relationship,the correlation coefficient R^(2)was 0.99,and the root mean square error was 0.178μg/g.Finally,a batch of pork samples with the same ractopamine content were prepared,and the device was used to detect ractopamine in pork.The predicted values had a good linear relationship with the standard physicochemical values of the samples,the correlation coefficient R^(2)was 0.99,and the root mean square error was 0.317μg/g.The device was simple,portable,inexpensive,and the detection time was less than 1 h.The detection limit was 1μg/g.It can be used for rapid screening of ractopamine in pork.
作者
郭庆辉
彭彦昆
庄齐斌
陈雅惠
GUO Qinghui;PENG Yankun;ZHUANG Qibin;CHEN Yahui(College of Engineering,China Agricultural University,Beijing 100083,China;National R&D Center for Agro⁃processing Equipment,Beijing 100083,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2022年第S02期278-284,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金国际(地区)合作交流项目(32020103016)
关键词
莱克多巴胺
瘦肉精
便携式快速筛查装置
表面增强拉曼散射
ractopamine
brown meat essence
portable rapid screening device
surface-enhanced Raman scattering
