摘要
目的探究肝囊型包虫病患者巨噬细胞极化及临床特点。方法将肝囊型包虫病患者和健康体检者分为实验组(n=30)与对照组(n=30)。采集两组临床资料,并对其血液生化学检测结果进行统计分析,采用流式细胞术检测外周血中CD14^(+)CD86^(+)M1型巨噬细胞和CD14^(+)CD206^(+)M2型巨噬细胞的表达水平,酶联免疫吸附试验检测血清中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-12(IL-12)、白细胞介素-10(IL-10)和转化生长因子β(TGF-β)水平。结果肝囊型包虫病患者血液生化学检测中,丙氨酸氨基转移酶(ALT)、门冬氨酸转移酶(AST)、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、球蛋白(GLB)、总胆红素(TBIL)和直接胆红素(DBIL)水平显著高于对照组,而白蛋白(ALB)和间接胆红素(IBIL)低于对照组,差异均有统计学意义(P<0.05)。肝囊型包虫病患者CD14^(+)CD206^(+)M2型巨噬细胞的表达水平(24.9±2.5)%明显高于对照组(20.16±2.65)%,差异有统计学意义(P<0.05)。肝囊型包虫病患者血清中TNF-α、IL-10和TGF-β表达水平高于对照组,IFN-γ水平低于对照组,差异均有统计学意义(P<0.05)。结论肝囊型包虫病患者肝功能受损且体内M1/M2的免疫失衡可能参与细粒棘球蚴的免疫逃逸反应。
Objective To explore the macrophage polarization and clinical characteristics in patients with hepatic cystic echinococcosis.Methods Patients with hepatic cystic echinococcosis and healthy people were divided into experimental group and control group,30 cases in each group.The clinical data of the two groups were collected and the blood biochemical test results were statistically analyzed.Flow cytometry was used to detect the expression levels of M1-polarized macrophages(CD14^(+)CD86^(+)M1)and M2-polarized macrophages(CD14^(+)CD206^(+)M2)of peripheral blood.The serum levels of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-12(IL-12),interleukin-10(IL-10)and transforming growth factor-β(TGF-β)were detected by enzyme-linked immunosorbent assay(ELISA).Results In the blood biochemical test of patients with hepatic cystic hydatid disease,the expression of alanine aminotransferase(ALT),aspartate transferase(AST),alkaline phosphatase(ALP),glutamyl transpeptidase(GGT),globulin(GLB),total bilirubin(TBIL)and direct bilirubin(DBIL)were significantly higher,while albumin(ALB)and indirect bilirubin(IBIL)were lower than those in the control group(P<0.05).The expression of M2-polarized macrophages(CD14^(+)CD206^(+)M2)in patients with hepatic cystic echinococcosis was significantly higher than that of control group(P<0.05).The expression of TNF-α,IL-10 and TGF-βin serum of patients with hepatic cystic echinococcosis were higher than those in control group,on the contrary,the expression of IFN-γwas lower(P<0.05).Conclusion In patients with hepatic cystic echinococcosis,the liver function was impaired and the immune imbalance of M1/M2 might be involved in the immune escape response of Echinococcus granulosus.
作者
张佳
董丹
杨靖
王二强
陈雪玲
ZHANG Jia;DONG Dan;YANG Jin;WANG Er⁃qiang;CHEN Xue⁃ling(School of Medicine,Shihezi University,Shihezi,Xinjiang 832002;Hepatobiliary Surgery,the First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,Xinjiang 832002,China)
出处
《热带医学杂志》
CAS
2022年第1期6-11,共6页
Journal of Tropical Medicine
基金
国家自然科学基金(82060297)
石河子大学2018年度自主资助支持校级科研项目(ZZZC201803A)
2019年自治区研究生科研创新项目(XJ2019G107)
2022年石河子大学大学生创新创业训练项目
