摘要
目的探讨血清嗜酸性粒细胞趋化因子-1(Eotaxin-1)、白细胞介素-35(IL-35)及钙结合蛋白S100A2表达水平对溃疡性结肠炎(UC)患者合并幽门螺旋杆菌(Hp)感染的评估价值。方法选取2017年2月-2020年2月辽宁省健康产业集团阜新矿总医院消化内科收治的活动期UC患者97例作为研究组,另选取非活动期UC患者50例作为对照组。采用酶联免疫吸附法(ELISA)、RT-PCR技术检测血清中Eotaxin-1、IL-35及S100A2 m RNA表达水平,采用14C呼气技术检测研究组患者Hp感染情况,采用受试者工作特征(ROC)曲线评估对溃疡性结肠炎合并Hp感染的诊断价值。结果研究组患者血清Eotaxin-1、S100A2 mRNA水平明显高于对照组,IL-35水平低于对照组,差异均有统计学意义(P<0.001)。研究组患者Hp感染阳性率为50.52%(49/97),高于对照组患者Hp感染阳性率28.00%(14/50),差异有统计学意义(χ2=6.830,P=0.009)。研究组中Hp阳性患者血清中Eotaxin-1、S100A2 mRNA水平明显高于阴性患者,IL-35明显低于阴性患者,差异均有统计学意义(P<0.001)。不同程度活动期UC患者血清中Eotaxin-1、IL-35、S100A2 mRNA水平差异有统计学意义(P<0.001);重度活动期患者血清Eotaxin-1、S100A2mRNA水平明显高于中度活动期、轻度活动期,IL-35水平低于中度活动期、轻度活动期,差异均有统计学意义(P<0.001);中度活动期患者血清Eotaxin-1、S100A2 mRNA水平高于轻度活动期,IL-35水平低于轻度活动期,差异均有统计学意义(P<0.001)。Eotaxin-1、IL-35、S100A2 mRNA对诊断UC合并Hp感染的曲线下面积(AUC)分别为0.782、0.813、0.734,三者联合诊断的AUC为0.956(95%CI:0.877~0.999),均高于单项诊断,差异有统计学意义(P<0.001)。结论活动期UC患者血清Eotaxin-1、IL-35、S100A2 m RNA水平与非活动期患者具有明显差异,且与是否感染Hp相关,可作为UC患者合并Hp感染的早期诊断指标及活动期严重程度的评价标准发挥临床作用。
Objective To explore the evaluation value of serum eosinophil chemokine-1(Eotaxin-1),interleukin-35(IL-35)and calcium binding protein S100A2 in patients with ulcerative colitis(UC)complicated with Helicobacter pylori(Hp)infection.Methods A total of 97 patients with active UC who were admitted to the Department of Gastroenterology in Fuxin Mine General Hospital of Liaoning Health Industry Group from February 2017 to February 2020 were selected as the study group,another 50 patients with UC in the inactive stage were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)and RT-PCR were used to detect serum Eotaxin-1,IL-35 and S100A2 mRNA expression level,14C breath technique was used to detect Hp infection in the study group.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of ulcerative colitis complicated with Hp infection.Results Serum Eotaxin-1 and S100A2mRNA levels in the study group were significantly higher than those in the control group(P<0.001),and IL-35 levels were lower than those in the control group(P<0.001).The positive rate of Hp infection in the study group was 50.52%(49/97)and the positive rate of Hp infection in the control group was 28.00%(14/50);their difference was statistically significant(χ2=6.830,P=0.009).The levels of Eotaxin-1 and S100A2 m RNA in the serum of Hp-positive patients in the study group were significantly higher than those of negative patients(P<0.001),while IL-35 was significantly lower than that of negative patients(P<0.001).The serum levels of Eotaxin-1,IL-35 and S100A2 mRNA in patients with different degrees of active UC were significantly different(P<0.001).Serum Eotaxin-1 and S100A2 m RNA levels in patients with severe active stage were significantly higher than those in moderate active stage and mild active stage(P<0.001),and serum IL-35 level in patients with severe active stage was significantly lower than that in moderate active stage and mild active stage(P<0.001).The serum levels of Eotaxin-1 and S100A2 mRNA in patients with moderate activity were significantly higher than those in mild activity,and the level of IL-35 in patients with moderate activity was significantly lower than that with mild activity(P<0.001).The area under curve(AUC)of Eotaxin-1,IL-35 and S100A2 mRNA for diagnosing UC complicated with Hp infection were0.782,0.813 and 0.734,respectively.The AUC of the three combined diagnosis was 0.956(95%CI:0.877-0.999),which was significantly higher than that of the single diagnosis(P<0.001).Conclusions The serum levels of Eotaxin-1,IL-35 and S100A2 mRNA in patients with active UC were significantly different from those in inactive patients,and were related to whether they were infected with Hp.These markers might play a clinical role as an early diagnostic index of UC patients complicated with Hp infection and be used as an evaluation standard for the severity of active stage.
作者
王欣欣
吴博
刘言
WANG Xin⁃xin;WU Bo;LIU Yan(Endoscope Center of Fuxin Mine General Hospital of Liaoning Health Industry Group,Fuxin,Liaoning 123000;Department of Gastroenterology,Fuxin Mine General Hospital of Liaoning Health Industry Group,Fuxin,Liaoning 123000,China)
出处
《热带医学杂志》
CAS
2022年第6期832-836,共5页
Journal of Tropical Medicine
基金
辽宁省科技厅基金项目(2017020367)
