摘要
目的探明补体在^(60)Coγ射线单次20 Gy胸部照射所致小鼠放射性肺损伤中的作用。方法^(60)Coγ射线单次20 Gy胸部照射C57BL/6小鼠,观察早期(15 d内)肺组织炎性反应和后期(30 d、180 d)肺纤维化,ELISA测定照射后1 d、3 d、7 d、15 d、30 d、180 d肺组织C2、C3a、C4、C5b-9含量,RT-PCR检测Beas-2B细胞照射后补体mRNA的表达。结果照射后小鼠放射性肺损伤表现为早期炎性反应和晚期纤维化。补体C2、C4和C5b-9复合物在照射后早期(3 d或7 d)增高(P<0.05),可能与照射引起的炎性反应有关。补体C3a在照射后3~180 d均明显高于对照组水平,提示其与放射性肺损伤关系密切。照射细胞中,补体C2、C3的mRNA表达增高,而C4、C5的mRNA水平无变化。结论不同补体在放射性肺损伤中的反应存在差异,其中补体C3a与放射性肺损伤关系极为密切,提示通过调控补体C3a及其受体可能是防治放射性肺损伤的新途径。
Objective To investigate the role of complement in radiation-induced lung injury in mice after chest irradiation with^(60)Coγ-rays at a single dose of 20 Gy.Methods C57BL/6 mice underwent chest irradiation with^(60)Coγ-rays at a single dose of 20 Gy,followed by observation for the inflammatory reaction of the lung tissue in the early stage(within 15 d)and pulmonary fibrosis in the later stage(30 and 180 d).Enzyme-linked immunosorbent assay was used to measure the levels of C2,C3a,C4,and C5b-9 in the lung tissues at 1,3,7,15,30,and 180 d after irradiation.The expression of complement mRNA in BEAS-2B cells after irradiation was determined using RT-PCR.Results Radiation-induced lung injury in micepresented as inflammatory response in the early stage and fibrosis in the late stage.Complement C2,C4,and C5b-9 complexes were increased in the early period(3 or 7 d)after irradiation(P<0.05),which might be associated with the inflammatory response induced by irradiation.During 3 to 180 d,complement C3a was significantly higher in the irradiated mice than in the control mice,suggesting a close relationship between C3a and radiation-induced lung injury.The irradiated cells showed increased mRNA expression of C2 and C3,with no changes in the mRNA levels of C4 and C5.Conclusion Different complement proteins have varying responses to radiation-induced lung injury,among which C3a is closely related to radiation-induced lung injury,suggesting that regulating C3a and its receptors may be a new way to prevent and treat radiationinduced lung injury.
作者
耿爽
李倩
郗停停
王美玉
郭浩鑫
王志鑫
毕晓光
鄢成名
杨陟华
王易龙
朱茂祥
GENG Shuang;LI Qian;XI Tingting;WANG Meiyu;GUO Haoxin;WANG Zhixin;BI Xiaoguang;YAN Chengming;YANG Zhihua;WANG Yilong;ZHU Maoxiang(Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing Key Laboratory for Radiobiology,Beijing 100850 China;Nanhua University,Hengyang 421001 China;College of Life Sciences,Hebei University,Baoding 071002 China)
出处
《中国辐射卫生》
2022年第5期535-541,共7页
Chinese Journal of Radiological Health
