摘要
以秋桑叶粉末为原料,采用超声辅助低共熔溶剂法提取桑叶多酚并研究其抗氧化活性。以20种低共熔溶剂对桑叶多酚进行提取,筛选出最优低共熔溶剂组合,在单因素试验的基础上,采用响应面分析法进一步优化桑叶多酚提取工艺参数;采用DPPH和ABTS自由基清除试验分析桑叶多酚低共熔溶剂提取物的抗氧化能力。结果表明:桑叶多酚最佳提取工艺为氯化胆碱/果糖/乙醇摩尔比1:1:2、含水量45%、液料比40 mL/g、超声功率360 W、超声温度40℃、超声时间40 min,在此条件下桑叶多酚提取量为76.82 mg/g;体外抗氧化结果表明,桑叶多酚的抗氧化作用与浓度呈正相关,对DPPH自由基和ABTS+自由基有显著的清除作用。
Using autumn mulberry leaf powder as raw material,the ultrasonic assisted deep eutectic solvents extraction method was applied to extract polyphenols.The optimal deep eutectic solvent composition was selected from among 20 types of deep eutectic solvents for extracting polyphenols from mulberry leaves.Based on single-factor experiments,the extraction process parameters were further optimized using response surface methodology,and the antioxidant capacity of mulberry leaf polyphenols in deep eutectic solvent extracts was analyzed by scavenging DPPH and ABTS+free radicals.The results showed that the optimal extraction conditions for mulberry leaf polyphenols were as follows:choline chloride/fructose/alcohol molar ratio of 1:1:2,water content of 45%,liquid-solid ratio of 40 mL/g,ultrasonic power of 360 W,ultrasonic temperature of 40℃,and an ultrasonic time of 40 min.Under these conditions,the amount of extracted polyphenols was 76.82 mg/g.In vitro antioxidant test results showed that the antioxidant ability of mulberry leaf polyphenols had a positive correlation with concentration and exhibited a significant scavenging effect on both DPPH free radicals and ABTS+free radicals.
作者
吴均
吴俊葶
赵珮
王梅
马婧秋
杨碧文
黄越
黄传书
WU Jun;WU Junting;ZHAO Pei;WANG Mei;MA Jingqiu;YANG Biwen;HUANG Yue;HUANG Chuanshu(Chongqing Sericulture Science and Technology Research institute,Chongqing 400700,China;Army Logistics University of PLA,Chongqing 401331,China)
出处
《食品科技》
CAS
北大核心
2024年第1期225-233,共9页
Food Science and Technology
基金
重庆市科研院所绩效激励引导专项(cstc2022jxjl00013)
关键词
低共熔溶剂
多酚
超声辅助
响应面
抗氧化活性
deep eutectic solvent
polyphenol
ultrasonic-assisted
response surface methodology
antioxidant activity
