摘要
相比于2D细胞模型和动物模型,类器官更能够重现来源器官的关键结构和功能特征,在生物医学领域得到了广泛的研究和运用。类器官芯片结合了类器官培养腔、微流控等多种功能单元,不仅可以根据研究者对靶器官的认知来设计仿生结构,模拟人体靶器官的复杂性;而且能控制并检测类器官所处微环境的变化,具有高通量和高灵敏度的特点。对类器官芯片的组成元件以及类器官芯片在医学研究中的应用,包括生物发育模型及疾病模型构建、药物研发、免疫评价等方面进行概述,并根据类器官芯片在目前研究应用中的不足进行讨论及展望,旨在为研究疾病或生物发育机理以及临床前研究提供有力的策略。
Compared with 2 D cell model and animal model,organoids can better reproduce the key structural and functional characteristics of the source organs,which have been widely studied and applied in the biomedical field.Organoids-on-a-chip combines organoid culture chamber,microfluidics and other functional units,which can not only be designed according to researchers’cognition of target organs,but also simulate the complexity of target organs.With the characteristics of high throughput and high sensitivity,it can control and detect the changes of the microenvironment in which organoids are located.This review summarizes the units and applications of organoids-on-a-chip in medical research,including construction of biological models and disease models,drug research and development,and immune evaluation,and discusses the shortcomings of organoids-on-a-chip in current research and application and proposes directions for future research.The aim is to provide a powerful strategy for the study of disease or biological development mechanism and preclinical research.
作者
冯晓莹
孟倩
陈巍
余磊
黄卫人
FENG Xiao-ying;MENG Qian;CHEN Wei;YU Lei;HUANG Wei-ren(State and Local Government Joint Engineering Laboratory of Synthetic Biology Medicine and Clinical Application of Key Technologies,Shenzhen Second Hospital,Shenzhen 518036,China;Shantou University Medical College,Shantou 515041,China;Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2022年第1期112-118,共7页
China Biotechnology
基金
国家重点研发计划(2019YFA0906000)
广东省自然科学基金(2020A1515010235)资助项目
关键词
类器官芯片
微流控
药物筛选
模型构建
免疫评价
Organoids-on-a-chip
Microfluidics
Drug screening
Construction of models
Evaluation of immune effect
