摘要
目的探讨基于超高效液相色谱(ultra performance liquid chromatography,UPLC)法,对细胞培养液中游离氨基酸浓度的测定方法。方法采用UPLC技术,结合优化的样品处理方法,对细胞培养液中游离氨基酸进行高效、精准的测定。结果该UPLC法能够快速、可靠地测定细胞培养液中的游离氨基酸浓度,为生物制药工业中细胞培养监控与优化提供了有效手段。结论对氨基酸浓度的准确测定,不仅有助于更好地了解细胞培养液中关键营养成分的动态变化,还为生产过程中的工艺优化提供了重要数据支持。
Objective:This study aims to explore a method for determining the concentration of free amino acids in cell culture media based on ultra performance liquid chromatography(UPLC).Methods Researchers used UPLC technology combined with optimized sample processing Methods to efficiently and accurately determine free amino acids in cell culture medium.Results The research results indicate that the UPLC method can quickly and reliably determine the concentration of free amino acids in cell culture medium,providing an effective means for monitoring and optimizing cell culture in the biopharmaceutical industry.Conclusion Accurate determination of amino acid concentration not only helps to better understand the dynamic changes of key nutrients in cell culture media,but also provides important data support for process optimization in the production process.
作者
田燕
王英超
王雅萍
TIAN Yan;WANG Ying-Chao;WANG Ya-Ping(Shandong Weigao Ruisheng Medical Equipment Co.,Ltd.,Weihai 250014,China)
出处
《实验室检测》
2024年第2期28-31,共4页
Laboratory Testing
关键词
超高效液相色谱法
氨基酸
细胞培养液
代谢分析
ultra high performance liquid chromatography
amino acids
cell culture medium
metabolism analysis
