摘要
目的采用超高效液相色谱法同时测定清肺汤中苦杏仁苷、桑皮苷A、栀子苷、芸香柚皮苷、橙皮苷、汉黄芩苷和黄芩素7个成分的含量,对清肺汤标准汤剂进行质量控制。方法采用Waters cortecs T3色谱柱(150 mm×2.1 mm,1.6μm),柱温35℃下以质量分数0.05%磷酸水(A)-乙腈(B)为流动相进行梯度洗脱(0~6 min,5%~6%B;6~22 min,6%~6.4%B;22~25 min,6.4%~13%B;25~45 min,13%~15%B;45~50 min,15%B;50~55 min,15%~16%B,55~60 min,16%~17%B;60~78 min,17%~23%B,78~82 min,23%~25%B;82~92 min,25%~50%B;92~95 min,50%~75%B;95~98 min,75%~90%B,98~103 min,90%B),流速为0.35 mL·min-1,DAD检测波长为210、239 nm。结果主要色谱峰完全分离且峰形良好,在考察范围内线性关系良好(r>0.999),具有较好的重复性和稳定性,加样回收率在92.3%~101.8%内。结论本实验作者所建立的方法为清肺汤汤标准剂质量的科学评价提供了依据,为清肺汤的开发和标准制定提供了参考。
Objective To establish an UHPLC-DAD method for simultaneous determination of 7 contents including amygdalin,mulberroside,geniposide,narirutin,hesperidin,wogonoside and baicalein in Qingfeitang as quality control.Methods The separation was performed on a Waters cortecs T3 column(150 mm×2.1 mm,1.6 μm) using UHPLC-DAD as the mobile phase was acetonitrile-water(containing 0.05% phosphoric acid) by gradient elution(0-6 min,5%-6%B;6-22 min,6%-6.4%B;22-25 min,6.4%-13%B;25-45 min,13%-15%B;45-50 min,15%B;50-55 min,15%-16%B;55-60 min,16%-17%B;60-78 min,17%-23%B,78-82 min,23%-25%B;82-92 min,25%-50%B;92-95 min,50%-75%B;95-98 min,75%-90%B;98-103 min,90%B) at flow rate of 0.35 mL·min-1.The detection wavelengths of DAD were 210 nm and 239 nm.Results The results show that all the peaks were well separated.All the components had a good linear relationship in the investigation range,respectively(r>0.999)The repeatability and stability were good and the recovery was between 91.3%-101.8%.Conclusion The developed method for assay of 7 components in Qingfeitang was simple,accurate and reliable.It provides the base for quality control of Qingfeitang.
作者
李佳慧
李清
LI Jiahui;LI Qing(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2020年第2期152-156,共5页
Journal of Shenyang Pharmaceutical University
