摘要
目的:采用正交试验对桑叶蛋白的提取工艺进行优化。方法:采用超声法提取桑叶中的蛋白,采用考马斯亮蓝法测定蛋白含量,以蛋白提取率为评价指标,设计6个单因素,依据单因素考察筛选出主要因素,并采用正交试验优化桑叶蛋白的提取工艺。结果:桑叶蛋白的最佳提取工艺:NaCl质量分数为0.3%,料液比为1∶35(g/mL),超声时间为25min,浸提时间为55min。在此提取工艺下验证3次,得到桑叶蛋白提取率分别为5.94%、6.01%、6.03%,平均提取率为6.01%,RSD为1.29%。结论:正交试验法优化得到的工艺可有效提高桑叶中蛋白的提取率,结果重复性好、稳定可行,为今后合理利用该资源提供实验依据。
Objective:To optimize the extraction process of mulberry leaf protein by orthogonal test.Methods:Ultrasonic method was used to extract the protein in mulberry leaves,and the protein content was determined by the Coomassie brilliant blue method.The protein extraction rate was used as the evaluation index.Six single factors were designed.The main factors were screened based on the single factor inspection and orthogonal test optimization Mulberry leaf protein extraction process.Results:The best extraction process of mulberry leaf protein was:NaCl mass fraction 0.3%,material-liquid ratio 1∶35(g/mL),ultrasonic time 25 min,extraction time 55 min.The extraction process was verified three times,and the mulberry leaf protein extraction rates were 5.94%,6.01%,6.03%,the average extraction rate was 6.01%,and the RSDwas 1.29%.Conclusion:The orthogonal test method can effectively improve the extraction rate of protein from mulberry leaves.The results are reproducible,stable and feasible,and provide experimental basis for the rational use of this resource in the future.
作者
刘苗苗
史锐
吴鹏
丛龙娇
Liu Miaomiao;Shi Rui;Wu Peng;Cong Longjiao(School of Pharmacy,Liaoninging University of Traditional Chinese Medicine,Shenyang 110000,China)
出处
《亚太传统医药》
2020年第12期63-66,共4页
Asia-Pacific Traditional Medicine
基金
辽宁省高校科研基金(L201938)
关键词
桑叶蛋白
正交试验
含量测定:考马斯亮蓝
Mulberry Leaf Protein
Orthogonal Test
Content Determination:Coomassie Brilliant Blue
