摘要
目的:观察放炎方对大鼠放射性肺损伤的治疗作用,观察大鼠肺组织病理改变及肺组织转化生长因子-β1(TGF--β1)、白介素-6(IL-6)表达水平,探讨放炎方的作用机制。方法:通过单次18Gy剂量、6MV-X线加速器胸部照射制备放射性肺损伤大鼠模型,随机分为模型组、地塞米松组,放炎方低剂量组、中剂量组、高剂量组,另设立未行照射的空白对照组,每组18只。各组大鼠于照射后第1天开始给予药物灌胃,每日1次,直至处死,分别于照射后2周、4周、6周随机选取各组大鼠5只,腹主动脉采血后处死,取肺组织行HE染色,观察肺组织病理变化,取血清样本检测TGF-β1、IL-6水平变化。结果:放炎方各剂量组大鼠放射性肺损伤病理学改变明显轻于模型组,第6周肺部炎症恢复优于模型组,大鼠血清TGF-β1、IL-6表达水平明显低于模型组,差异均具有统计学意义(P<0.01)。结论:放炎方能减轻放射性肺损伤大鼠的肺部炎症,减少渗出,可能的作用机制是降低TGF-β1、IL-6因子的表达水平。
Objective:To observe Fangyan decoction for prevention and control of radioactive lung injury,observe the lung tissue pathology change and the expression of TGF-β1,IL-6,discuss Fangyan decoction effection mechanism.Methods:SD rats were given single irradiation linac in 18 Gy to build models,were randomLy divided into model group,dexamethasone group,low dose Fangyan decoction group,middle dose group,high dose group,and blank control group without irradiation,each group of 18.The rats were given different drug at the first day after irradiation,1 times a day,until death,respectively in 2 weeks,4 weeks,6 weeks after irradiation randomLy selected 5 rats from each group,killed rats after taking blood form abdominal aorta,and HE dyeing to observate pathology change of lung tissue,ELISA to detect serum specimen TGF-β1,IL-6 dynamically.Results:The radioactive lung injury pathological change of the Fangyan decoction intervention groups ignificantly lighter than model group,lung inflammation recovery is better than the model group at 6 weeks,the serum expression of TGF-β1,IL-6 is significantly lower than the model group(P<0.01).Conclusion:Fangyan decoction can alleviate radioactive rat lung inflammation,reduce inflammation effusion,the mechanism may be to reduce the expression of TGF-β1,IL-6.
作者
刘伟
兰东强
王柱
唐伟智
冯叶超
牛俊杰
简小兰
Liu Wei;Lan Dongqiang;Wang Zhu;Tang Weizhi;Feng Yechao;Niu Junjie;Jian Xiaolan(The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,China)
出处
《亚太传统医药》
2021年第2期18-21,共4页
Asia-Pacific Traditional Medicine
基金
湖南省中医药研究院项目(201707,201802)
国家自然科学基金(81904109)
湖南省自然科学基金(2019JJ50344)
