摘要
目的系统研究复方伤痛胶囊的主要化学成分,并探讨其发挥药效的主要作用机制,为其药效物质研究提供一定的参考依据。方法采用UHPLC-Q-Orbitrap HRMS对复方伤痛胶囊的主要化学成分进行全面分析,色谱柱采用Waters Acquity UPLC? BEH C18色谱柱(50 mm×2.1 mm,1.7μm),流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱,体积流量为0.2 mL/min。根据化合物的一级、二级质谱信息,并与对照品或参考文献进行比对,以实现对药物的化学信息进行快速、精准识别。在此基础上,采用网络药理学方法对药物的化学成分进行靶标分析、功能富集,初步筛选出药物的主要药效物质,并探讨其作用机制。结果从复方伤痛胶囊中共鉴定出36个化学成分,靶标功能富集分析表明该药主要从调节血管内皮、血管平滑肌、影响血小板功能、促进能量供应、减轻炎症反应以及缓解疼痛等过程,从而发挥其活血化瘀、行气止痛的治疗作用。结论本研究采用UHPLC-Q-Orbitrap HRMS高分辨质谱分析结合网络药理学的方法,为复方伤痛胶囊的有效成分识别、质量标志物筛选以及潜在作用机制研究提供了科学的理论依据和重要参考。
Objective To systematically study the main chemical components of Fufang Shangtong Capsule and explore the main mechanism of its effect, and provide some reference for the research of its pharmacodynamic substance. Methods In this study, UHPLC-Q-Orbitrap HRMS was used to comprehensively analyze the main chemical components of Fufang Shangtong Capsule. The chromatographic column was Waters Acquity UPLC? BEH C18 chromatographic column(50 mm ×2.1 mm, 1.7 μm) and the mobile phase was acetonitrile(A)-0.1% formic acid water(B). According to the MS/MS spectrometry information of compounds, and the comparison with standards or references, the chemical information of drugs can be quickly and accurately identified. On this basis, the network pharmacology method was used to analyze the chemical composition target of the drug, enrich its function, preliminarily select the main effective substances of the drug, and simultaneously explore its mechanism of action. Results A total of 36 chemicals were identified in this study from Fufang Shangtong Capsule. The target function of enrichment analysis showed that the drug mainly played its therapeutic effect on regulating vascular endothelial, vascular smooth muscle pain, affecting platelet function, promoting energy supply, reducing inflammation and relieving pain, so as to exert its efficacy in promoting blood circulation and removing stasis, invigorating qi and relieving pain. Conclusion In this study, UHPLC-Q-Orbitrap HRMS combined with network pharmacology was used, wich provided scientific theoretical basis and important reference for the identification of effective ingredients, screening of quality markers and the study of potential mechanism of action of Fufang Shangtong Capsule.
作者
曹辉
王肖辉
李卓伦
李震
左莉华
金建文
武婧
康建
周霖
孙志
CAO Hui;WANG Xiao-hui;LI Zhuo-lun;LI Zhen;ZUO Li-hua;JIN Jian-wen;WU Jing;KANG Jian;ZHOU Lin;SUN Zhi(Department of Endovascular Surgery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Ultrasonography,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Pharmacy,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Henan Precision Medicine Clinical Mass Spectrometry Engineering Research Center,Zhengzhou 450052,China;Shaanxi Licai Pharmaceutical Co.,Ltd.,Xianyang 712000,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第3期599-606,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81600377)
河南省高等学校重点科研项目基础研究计划(19A320070).
