摘要
研究羌活根状茎(竹节羌)生长发育过程及碳水化合物、内源激素与次生代谢产物的变化规律,对人工栽培下形成羌活优质药材商品具有重要指导意义。将一年生羌活实生苗移栽至原生境,动态监测羌活生长及生理生化的变化过程。结果表明:①春天移栽至原生境的羌活当年即可形成竹节羌;②移栽60 d后,羌活地下部分根长、根粗快速增加,根、根状茎中碳水化合物含量快速积累;移栽120 d后,羌活地下部分根和根状茎生长缓慢,根、根状茎中淀粉含量持续增加,蔗糖、可溶性总糖含量逐渐减少;③羌活根状茎中脱落酸(ABA)含量先减少后增加,生长素(IAA)含量先维持稳定后快速增加,赤霉素(GA3)、玉米素核苷(ZR)含量则持续增加;④羌活根状茎(竹节羌)的羌活醇含量显著高于根的对应值,异欧前胡素含量则显著低于根的对应值,但根状茎中二者的总含量显著高于根。因此,野生抚育下羌活可形成次生代谢产物含量较高的根状茎,且根状茎的生长发育与碳水化合物的变化密切相关,并受到相关内源激素的调控。
Study the growth and development process of rhizomes(bamboo-like part)of Notopterygium incisum and the changes of carbohydrate,endogenous hormones and secondary metabolites,and provide theoretical guidance for the formation of high-quality N.incisum medicinal commodities under artificial cultivation.The One-year-old seedlings were transplanted to the original habitat,and the growth and physiological characteristics of N.incisum were dynamically monitored.The results showed that:①Seedlings transplanted to the original habitat in spring could form rhizomes(bamboo-like part)in the same year.②After 60 days of transplantation,the root length and root diameter of underground part of N.incisum had increased rapidly,and carbohydrate content in roots and rhizomes had accumulated rapidly.After 120 days of transplantation,the roots and rhizomes of underground part had grown slowly,and starch content in roots and rhizomes increased continuously,while sucrose and total soluble sugar content decreased gradually.③The content of abscisic acid(ABA)in rhizomes decreased firstly and then increased,while the indole acetic acid(IAA)content stabilized firstly and then increased rapidly,and the contents of gibberellin(GA3)and zeatin riboside(ZR)continued to increase.④The content of notopterol in rhizomes was higher than that in roots,while the content of isoimperatorin was lower than that in roots,but the total content of the both in rhizomes was higher than that in roots.Therefore,N.incisum can form rhizomes with high content of secondary metabolites under wild tending,and the growth and development of rhizomes are closely related to changes in carbohydrates and are regulated by related endogenous hormones.
作者
杨萍
王红兰
孙辉
朱文涛
邱涛
杜玖珍
孙洪兵
周毅
蒋舜媛
YANG Ping;WANG Hong-lan;SUN Hui;ZHU Wen-tao;QIU Tao;DU Jiu-zhen;SUN Hong-bing;ZHOU Yi;JIANG Shun-yuan(Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;Department of Environmental Science and Engineering,Sichuan University,Chengdu 610065,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第4期739-745,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2017YFC1700705)
国家自然科学基金项目(81473311)
四川省科技厅重点研发项目(2018SZ0056)
2017年中医药公共卫生服务补助专项(财社[2017]66号)
2018年中医药公共卫生服务补助专项(财社[2018]43号).
关键词
羌活
根状茎
碳水化合物
内源激素
次生代谢产物
Notopterygium incisum
rhizome
carbohydrate
endogenous hormone
secondary metabolites
