摘要
为了解鸭梨花香的生理生态功能,采用顶空固相微萃取(HS-SPME)与气质联用(GC-MS)相结合的方法对鸭梨花朵香气成分进行分析,比较了65μm PDMS/DVB和50/30μm DVB/CAR/PDMS 2种不同涂层纤维的萃取头对鸭梨花朵香气成分的萃取效果和特点。结果表明,65μm PDMS/DVB和50/30μm DVB/CAR/PDMS 2种萃取头检测出的挥发物数量和种类分别为20种和41种、5类和10类物质,其中65μm PDMS/DVB萃取头检测出的挥发性成分以烷烃类的种类和含量最高(烷烃类7种,38.63%),而50/30μm DVB/CAR/PDMS萃取头检测出的挥发物以烯类(烯类7种,41.16%)和醇类(醇类7种,24.11%)为主要成分。根据萃取化合物的数量、种类和含量,50/30μm DVB/CAR/PDMS萃取头更适于提取分析鸭梨花朵的香气成分。
To understand the physiological and ecological function of pear flower scents,the volatile compounds of Pyrus bretschneideri flowers were analyzed by headspace solid phase microextraction(HS-SPME)and GC-MS.The extraction effects and characteristics of 65μm PDMS/DVB and 50/30μm DVB/CAR/PDMS were compared.Results showed that the number and type of volatile compounds of pear flowers detected by 65μm PDMS/DVB and 50/30μm DVB/CAR/PDMS extraction heads were respectively 20 and 41 species,5 and 10 types of substances,of which,alkane had the most kinds and the highest content(7 types,38.63%)among the volatile organic compounds detected by 65μm PDMS/DVB extraction heads,while alkenes(7 types,41.16%)and alcohols(7 types,24.11%)were the main compounds detected by 50/30μm DVB/CAR/PDMS extraction heads.According to the number,type and content of extracted compounds,the 50/30μm DVB/CAR/PDMS extraction heads are more suitable for extracting and analyzing the volatile compounds of pear flowers.
作者
马卫华
李立新
申晋山
武文卿
宋怀磊
张旭凤
李捷
Ma Weihua;Li Lixin;Shen Jinshan;Wu Wenqing;Song Huailei;Zhang Xufeng;Li Jie(Institute of Horticulture,Shanxi Academy of Agricultural Sciences,Taiyuan 030031)
出处
《中国农学通报》
2020年第4期147-150,共4页
Chinese Agricultural Science Bulletin
基金
山西省农业科学院博士基金“蜜蜂对梨花挥发物的嗅觉行为研究”(YBSJJ1713)
现代农业产业技术(蜜蜂)体系建设“设施作物授粉技术研究”(CARS-44-KXJ23).
关键词
鸭梨花
顶空固相微萃取
气相色谱-质谱联用
萃取头
挥发性成分
Pyrus bretschneideri flowers
headspace solid phase microextraction
gas chromatography-mass spectrometry
extraction heads
volatile components
