摘要
浙江省金华市和丽水市出现了会导致茭白叶鞘产生褐色斑点的病害,感染该病害的植株会产生短而发黄的茭白。采用组织分离法从病样中分离得到XYQ3菌株。通过形态学观察、多序列比对分析和致病性试验,确定茭白叶鞘病的病原菌为Microdochium seminicola。在实验室条件下,对田间常用的12种杀菌剂进行毒性试验,结果表明,250 g·L^(-1)咪鲜胺乳油对病原菌菌丝体的抑制效果最好,EC_(50)值为0.067 mg·L^(-1);其次是300 g·L^(-1)苯并丙环唑乳油和60%苯醚甲环唑WG,EC_(50)值分别为0.373和1.083 mg·L^(-1)。综合考虑,250 g·L^(-1)咪鲜胺乳油为最佳杀菌剂,50%异菌脲可湿性粉剂可作为替代品。本研究为茭白叶鞘褐斑病的鉴定和防治提供了科学依据。
Some diseases cause the leaf sheath of Zizania latifolia to produce brown spots and the infected plants produce short and yellowish Jiaobai in Jinhua City and Lishui City,Zhejiang Province.XYQ3 strain was obtained from diseased samples by tissue isolation.The pathogen causing leaf sheath disease of Z.latifolia was identified as Microdochium seminicola by morphological characterization,sequence alignment analysis and pathogenicity test.Toxicity test of 12 fungicides commonly used in fields was carried out in laboratory conditions.The results showed that the inhibition effect on mycelium was best for prochloraz EC at 250 g·L^(-1),with an EC_(50)of 0.067 mg·L^(-1);followed by 300 g·L^(-1)Benzo propiconazole EC and 60%Difenoconazole WG,with EC_(50)of 0.373 and 1.083 mg·L^(-1),respectively.In comprehensive consideration,250 g·L^(-1)Prochloraz EC is the best as a fungicide agent,and 50%Iprodione WP can be used as an alternative.This study provided scientific basis for the identification and control of leaf sheath brown spot of Z.latifolia.
作者
李怡鹏
郑寨生
宋瑞琪
杨梦飞
LI Yipeng;ZHENG Zhaisheng;SONG Ruiqi;YANG Mengfei(Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation,Jinhua Academy of Agricultural Sciences,Jinhua 321000,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第3期485-490,共6页
Acta Phytopathologica Sinica
基金
财政部和农业农村部国家现代农业产业技术体系项目(CARS-24-09)
浙江省农业(蔬菜新品种选育)新品种选育重大科技专项(2021C02065)
金华市科技计划项目(2020-2-010)
