Background: The restrictive lockdown measures that were introduced as a response to the COVID-19 pandemic imposed constraints that affected university students’ drinking habits and mental health. Methods: The current...Background: The restrictive lockdown measures that were introduced as a response to the COVID-19 pandemic imposed constraints that affected university students’ drinking habits and mental health. Methods: The current study evaluated the rate of binge drinking among university students as they returned to normal life post-lockdown and whether binge drinking affected their mental health and quality of life. A cross-sectional study included 1382 university students (18 - 24 years) of one private Southwestern university in Brazil. Alcohol Use Disorders Identification, the Beck Depression Inventory-II, and the World Health Organization Quality of Life Brief were used. Data were collected after the COVID-19 pandemic lockdown period (from October 2021 to May 2022). Results: 52.6% of the students reported binge drinking, and 35.6% reported symptoms of depression. Binge drinkers were 1.41 times more likely to experience depressive symptoms when compared with non-binge drinkers, and binge drinking was a risk factor for lower quality of life in all three domains (physical, psychological and environmental). Conclusion: Our study highlights an increased prevalence of binge drinking in university students after the pandemic lockdown. The rise in binge drinking rates was linked to depressive symptoms and worsening of quality of life.展开更多
Climate change is a reality. The burning of fossil fuels from oil, natural gas and coal is responsible for much of the pollution and the increase in the planet’s average temperature, which has raised discussions on t...Climate change is a reality. The burning of fossil fuels from oil, natural gas and coal is responsible for much of the pollution and the increase in the planet’s average temperature, which has raised discussions on the subject, given the emergencies related to climate. An energy transition to clean and renewable sources is necessary and urgent, but it will not be quick. In this sense, increasing the efficiency of oil extraction from existing sources is crucial, to avoid waste and the drilling of new wells. The purpose of this work was to add diffusive and dispersive terms to the Buckley-Leverett equation in order to incorporate extra phenomena in the temporal evolution between the water-oil and oil-water transitions in the pipeline. For this, the modified Buckley-Leverett equation was discretized via essentially weighted non-oscillatory schemes, coupled with a three-stage Runge-Kutta and a fourth-order centered finite difference methods. Then, computational simulations were performed and the results showed that new features emerge in the transitions, when compared to classical simulations. For instance, the dispersive term inhibits the diffusive term, adding oscillations, which indicates that the absorption of the fluid by the porous medium occurs in a non-homogeneous manner. Therefore, based on research such as this, decisions can be made regarding the replacement of the porous medium or the insertion of new components to delay the replacement.展开更多
The identification of natural, plant-derived compounds with pesticidal properties is crucial for developing environmentally sustainable alternatives to synthetic pesticides. In this study, four major lignans—dihydroc...The identification of natural, plant-derived compounds with pesticidal properties is crucial for developing environmentally sustainable alternatives to synthetic pesticides. In this study, four major lignans—dihydroclusin, cubebin, clusin, and yatein—were isolated from the crude extract of Piper cubeba fruit. Phytotoxicity assays revealed herbicidal activity against Agrostis stolonifera, with dihydroclusin and clusin exhibiting the highest efficacy, inhibiting seed germination by 50% and showing IC50 values of 2.9 µM and 45 µM, respectively, against Lemna paucicostata. Additionally, all compounds, except dihydroclusin, demonstrated fungicidal activity against the strawberry anthracnose pathogen Colletotrichum fragariae. Moreover, only dihydroclusin exhibited larvicidal activity against Aedes aegypti, causing 96% mortality of mosquito larvae at the 100-ppm concentration tested. These findings highlight the broad-spectrum bioactivity of Piper cubeba lignans, suggesting their potential as alternative agents of synthetic pesticides for managing agricultural pests.展开更多
The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for lit...The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.展开更多
The expression of miRNAs is associated with a variety of diseases, including neoplasms. In recent years, a large number of abnormally expressed miRNAs have been shown to be effective in understanding the oncogenesis, ...The expression of miRNAs is associated with a variety of diseases, including neoplasms. In recent years, a large number of abnormally expressed miRNAs have been shown to be effective in understanding the oncogenesis, development, progression and prognosis of meningiomas. Furthermore, it is known that miRNAs act as oncogenes or tumor suppressors and that they regulate essential molecular pathways such as transcription factors involved in the pluripotency phenotype of stem cells. Therefore, the aim of this study was to analyze the expression of microRNAs miR-34a, miR-145 and miR-221 that regulate the pluripotency pathway of stem cells and correlate with tumor recurrence in grade I meningiomas. We used 30 samples, belonging to 15 patients who presented recurrences of grade I meningiomas. We observed low expression levels of miR-34a in the group of tumor recurrences when compared to control individuals and primary tumors, which may be associated with the tumor suppressor role of this miR. The miR-145 also showed decreased expression levels between the control group and the group of tumor recurrences. We also observed decreased expression levels in miR-145 between the control group and the primary tumors group. MiR-221 did not differ between the studied groups. MiR-34a and miR-145 microRNAs that regulate the stem cell pluripotency pathway are shown to be hypo expressed in tumor recurrences of grade I meningiomas and are shown to be good candidates for prognosis and recurrence biomarkers in meningiomas.展开更多
Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram...Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity.In our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus formation.Calluses were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf sheath.The secondary embryogenesis was formed in yellow-globular callus.The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis.Glucose was more efficiency in the presence of light.Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.展开更多
AIM: To evaluate the effects of protein deprivation on the myenteric plexus of the esophagus of weanling rats. METHODS: Pregnant female Wistar rats were divided into 2 groups: nourished (N),receiving normal diet,and u...AIM: To evaluate the effects of protein deprivation on the myenteric plexus of the esophagus of weanling rats. METHODS: Pregnant female Wistar rats were divided into 2 groups: nourished (N),receiving normal diet,and undernourished (D),receiving a protein-deprived diet,which continued after birth. At twenty-one days of age,13 esophagi from each group were submitted to light microscopy and morphometrical analysis employing the NADH diaphorase,NADPH diaphorase and acetylcholinesterase techniques. Three other esophagi from each group were evaluated with transmission electron microscopy (TEM). RESULTS: In both the NADH- and the NADPH-reactive mounts,the neurons of the N mounts were more intensely stained,while in the D esophagi only the larger neurons were reactive. Many myenteric neurons of N were intensely reactive for AChE activity but only a few neurons of D exhibited these aspects. Ultrastructural analysis revealed that the granular reticulum of N showed large numbers of ribosomes aligned on the outer surface of its regularly arranged membrane while the ribosomes of D were disposed in clusters. The chromatin was more homogeneously scattered inside the neuron nucleus of N as well as the granular component of the nucleolus was evidently more developed in this group. Statistically significant differences between N and D groups were detected in the total estimated number of neurons stained by the NADPH technique. CONCLUSION: The morphological and quantitative data shows that feeding with protein-deprived diet in 21-d old rats induces a delay in the development of the myenteric neurons of the esophagus.展开更多
AIM： To evaluate the potential of S-nitroso-N-acetylcysteine （SNAC） in inhibition of lipid peroxidation and the effect of oral SNAC administration in the prevention of nonalcoholic fatty liver disease （NAFLD） in ...AIM： To evaluate the potential of S-nitroso-N-acetylcysteine （SNAC） in inhibition of lipid peroxidation and the effect of oral SNAC administration in the prevention of nonalcoholic fatty liver disease （NAFLD） in an animal model.METHODS： NAFLD was induced in Wistar male rats by choline-deficient diet for 4 wk. SNAC-treated animals （n=6） （1.4 mg/kg/day of SNAC, orally） were compared to 2 control groups： one （n=6） received PBS solution and the other （n=6） received NAC solution （7 mg/kg/d）. Histological variables were semiquantitated with respect to macro and microvacuolar fat changes, its zonal distribution, foci of necrosis, portal and perivenular fibrosis, and inflammatory infiltrate with zonal distribution. LOOHs from samples of liver homogenates were quantified by HPLC. Nitrate levels in plasma of portal vein were assessed by chemiluminescence. Aqueous low-density lipoprotein （LDL） suspensions （200 pg protein/mL） were incubated with CuCl2 （300 μmol/L） in the absence and presence of SNAC （300 μmol/L） for 15 h at 37 ℃ Extent of LDL oxidation was assessed by fluorimetry. Linoleic acid （LA） （18.8 μmol/L） oxidation was induced by soybean lipoxygenase （SLO） （0.056 μmol/L） at 37 ℃ in the presence and absence of N-acetylcysteine （NAC） and SNAC （56 and 560 pmol/L） and monitored at 234 nm. RESULTS： Animals in the control group developed moderate macro and microvesicular fatty changes in periportal area. SNAC-treated animals displayed only discrete histological alterations with absence of fatty changes and did not develop liver steatosis. The absence of NAFLD in the SNAC-treated group was positively correlated with a decrease in the concentration of LOOH in liver homogenate, compared to the control group （0.7±0.2 nmol/mg vs 3.2±0.4 nmol/mg protein, respectively, P〈0.05）, while serum levels of aminotransferases were unaltered. The ability of SNAC in preventing lipid peroxidation was confirmed in in vitro experiments using LA and LDL as model substrates. CONCLUSION： Oral administration of SNAC prevents the onset of NAFLD in Wistar rats fed with cholinedeficient diet. This effect is correlated with the ability of SNAC to block the propagation of lipid peroxidation in vitro and in vitro.展开更多
The chemical composition of marabou（Dichrostachys cinerea） wood and its treatment with acetic acid were investigated.Two different treatment approaches,direct acetosolv and combined acid prehydrolysis/acetosolv,were...The chemical composition of marabou（Dichrostachys cinerea） wood and its treatment with acetic acid were investigated.Two different treatment approaches,direct acetosolv and combined acid prehydrolysis/acetosolv,were evaluated.The effects of acetic acid concentration（50%,70% and 90%） and temperature（normal boiling temperature and 121°C） on yield of solids,solubilization of lignin and hemicelluloses and recovery of cellulose were evaluated for both treatments.High solubilization of marabou components was observed in the direct acetosolv treatment at 121°C,especially at the highest acetic acid concentration,where around 84.8% of lignin and 78% of hemicelluloses were removed.When the material was subjected to acid prehydrolysis prior to acetosolv treatment,lignin solubilization was improved,especially at low acetic acid concentrations.Above 80% of the solubilized lignin was recovered from the liquors in the direct acetosolv treatment,but the recovery was lower in the combined treatment.Cellulose was well preserved in all the treatment schemes.展开更多
The article contains a list of 25 structured technical sheets on fruits and pseudo-fruits that will serve as guidance in improving the quality of health of people, whether with chronic diseases or not. The technical s...The article contains a list of 25 structured technical sheets on fruits and pseudo-fruits that will serve as guidance in improving the quality of health of people, whether with chronic diseases or not. The technical sheets contain data related to the composition, active ingredients, interaction with medication, health conditions in which they may be useful in health care by various health professionals and references used to structure the material referring to each fruit or pseudo-fruit.展开更多
During the production of nitrile rubber, significant amounts of nitrogen in the form of ammonium are generated in the wastewater. The discharge of this high-nitrogen wastewater can lead to serious environmental issues...During the production of nitrile rubber, significant amounts of nitrogen in the form of ammonium are generated in the wastewater. The discharge of this high-nitrogen wastewater can lead to serious environmental issues, including eutrophication, disruption of aquatic ecosystems, and groundwater contamination. To mitigate these impacts, this research explored the bioremediation capabilities of the macroalgae Ulva lactuca (Chlorophyta) for removing nitrogen from nitrile rubber production wastewater. The study employed single-phase and Michaelis-Menten decay models based on ammonium consumption, using various dilutions of wastewater to identify the optimal concentration for treatment. The physiological state of the macroalgae was monitored by measuring the photosynthetic capacity and specific growth rate during the experiments. In the presence of U. lactuca, ammonium concentrations decreased in all treatment groups, confirming that the ammonium kinetics conformed to both applied models. Our results show that U. lactuca effectively reduces ammonium concentrations, with an approximate removal rate of 0.020 µM·g−1·min−1 across different wastewater concentrations (70%, 80%, 90%, and 100%). Notably, the treatments with 70%, 80%, and 90% wastewater strength achieved about 67% reduction in ammonium, demonstrating the alga’s capacity to treat high-nitrogen wastewater. The photosynthetic performance of U. lactuca initially declined in control conditions but stabilized across all treatments, highlighting its adaptability. The kinetic analysis using the Michaelis-Menten model indicated a Vmax of 1342 μM·g−1·DMh−1, suggesting a robust capacity for ammonium uptake when fully saturated. Our study underscores the potential of Ulva lactuca as a cost-effective and efficient agent for wastewater bioremediation, particularly in settings with high nitrogen loads.展开更多
This study investigated the effects of yerba mate consumption, a South American beverage, on cardiac remodeling in rats. For this purpose, 24 male Wistar rats were divided into Control Group (CG) which received filter...This study investigated the effects of yerba mate consumption, a South American beverage, on cardiac remodeling in rats. For this purpose, 24 male Wistar rats were divided into Control Group (CG) which received filtered water and a standard diet, and Yerba Mate Group (YM), 6 g of Ilex paraguariensis in 100 ml water and the same diet, for 30 days. The YM group showed a reduction in final body weight and food consumption without altering weight gain. Regarding cardiac remodeling, the YM group exhibited a decrease in the right ventricle weight/final body weight ratio, suggesting cardiac atrophy, without affecting the atria and left ventricle. There was no change in cardiomyocyte area or nuclear fractal dimension in both groups. However, animals that consumed yerba mate showed increased collagen deposition and a smaller fractal dimension in the left ventricle. The consumption of yerba mate at room temperature for 30 days induced changes in cardiac remodeling, as evidenced by increased collagen deposition and alterations in fractal dimension in the left ventricle.展开更多
Togo’s economy is heavily dependent on rainfed agriculture. Therefore, anomalies in precipitation can have a significant impact on crop yields, affecting food production and security. Thus, monitoring anomalous clima...Togo’s economy is heavily dependent on rainfed agriculture. Therefore, anomalies in precipitation can have a significant impact on crop yields, affecting food production and security. Thus, monitoring anomalous climate conditions in Togo through the combination of precipitation satellite-based data and Standard Precipitation Index (SPI) help anticipate the development of drought scenarios or excessive rainfall, allowing farmers to adjust their strategies and minimize losses. Continuous and adequate spatial monitoring of these climate anomalies provided by satellite-based products can be central to an effective early warning system (EWS) implementation in Togo. Precipitation satellite-based products have been presented invaluable tools for assessing droughts and , offering timely and comprehensive data that supports a wide range of applications. In this study, we applied the Integrated Multi-satellite Retrievals for GPM (IMERG) rainfall product, a unified satellite global precipitation product developed by NASA, to identify and characterize the severity of dry and wet climate events in Togo during the period from 2001 to 2019. The Standard Precipitation Index (SPI), as the main index recommended by the World Meteorological Organization to monitor drought wide world, was selected as the reference index to monitor dry and wet climate events across Togo regions. The results show two distinct major climate periods in Togo in the timeframe analyzed (2001-2019), one dominated by wet events from 2008 to 2010, and a second marked by severe and extreme dry events from 2013 to 2015;MERG rainfall and SPI combination were able to capture these events consistently.展开更多
Obesity is a highly prevalent disease worldwide, associated with substantial economic and social costs. Besides the traditional treatment approach with low-calorie diet and regular physical exercise, some adjunct ther...Obesity is a highly prevalent disease worldwide, associated with substantial economic and social costs. Besides the traditional treatment approach with low-calorie diet and regular physical exercise, some adjunct therapies have been investigated to assist body fat loss in obese patients. Carboxytherapy is an invasive intervention already studied for a range of conditions, from wound healing to the treatment of abdominal adiposity, due to its oxidative effect on adipocytes, in addition to contributing to improved skin elasticity. Thus, this study aimed to investigate the efficacy and safety of carboxytherapy in reducing abdominal circumference in patients hospitalized for obesity treatment. Fifty-eight adult inpatients with obesity underwent an individualized treatment (low-calorie diet and daily physical exercise for 40 - 90 days) associated with a mean of 11 sessions of carboxytherapy in the abdominal region and were assessed for body mass index (BMI), abdominal circumference (AC), waist to hip ratio (WHR) and percent body fat (%BF). After the intervention, significant reductions were observed in all studied variables (p < 0.001). The adherence rate was 53.67%, and absences were mainly related to the discomfort caused by the sessions. However, no important side effects were registered. Carboxytherapy was a safe intervention for obese patients, and when employed in combination with an inpatient program involving a low-calorie diet and regular exercise, contributed to the reduction of abdominal adiposity.展开更多
Bariatric and metabolic surgeries have gained extensive popularity and trust due to their documented efficacy and safety in managing not only obesity but also associated comorbidities such as diabetes mellitus, hypert...Bariatric and metabolic surgeries have gained extensive popularity and trust due to their documented efficacy and safety in managing not only obesity but also associated comorbidities such as diabetes mellitus, hypertension, dyslipidemia, sleep apnea, and joint pain. Traditionally, bariatric surgeries have been categorized into hypoabsorptive, restrictive, or hybrid approaches. However, these classifications inadequately reflect the complex anatomical and physiological alterations associated with modern surgical methodologies. This paper explores the evolution of metabolic surgeries, emphasizing the integration of physiological concepts into classic procedures to provide more tailored and effective treatment options for obesity and its comorbidities. Finally, the proposal for a new classification based on current metabolic concepts will facilitate communication among patients, doctors, and healthcare professionals. Additionally, it will enable a more didactic and standardized approach to data collection for conducting studies and publications.展开更多
AIM：To compare narrow-band imaging（NBI）without image magnification,and chromoendoscopy with Lugol＇s solution for detecting high-grade dysplasia and intramu-cosal esophageal squamous cell carcinoma（SCC）in patient...AIM：To compare narrow-band imaging（NBI）without image magnification,and chromoendoscopy with Lugol＇s solution for detecting high-grade dysplasia and intramu-cosal esophageal squamous cell carcinoma（SCC）in patients with head and neck cancer.METHODS：This was a prospective observational study of 129 patients with primary head and neck tumors consecutively referred to the Gastrointestinal Endoscopy Unit of Hospital das Clínicas,Sao Paulo University Medical School,Brazil,between August 2006 and Feb-ruary 2007.Conventional examinations with NBI and Lugol chromoendoscopy were consecutively performed,and the discovered lesions were mapped,recorded and sent for biopsy.The results of the three methods were compared regarding sensitivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood value and negative likelihood value.RESULTS：Of the 129 patients,nine（7%）were diag-nosed with SCC,5 of which were in situ and 4 which were intramucosal.All carcinomas were detected through NBI and Lugol chromoendoscopy.Only 4 le-sions were diagnosed through conventional examination,all of which were larger than 10 mm.CONCLUSION：NBI technology with optical filters has high sensitivity and high negative predictive value for detecting superficial esophageal SCC,and produces results comparable to those obtained with 2.5%Lugol chromoendoscopy.展开更多
AIM: To investigate the systemic hemodynamic effects of two surgical procedures largely employed for treatment of schistosomal portal hypertension. METHODS: Thirty-six patients undergoing elective surgical treatment o...AIM: To investigate the systemic hemodynamic effects of two surgical procedures largely employed for treatment of schistosomal portal hypertension. METHODS: Thirty-six patients undergoing elective surgical treatment of portal hypertension due to hepatosplenic mansonic schistosomiasis were prospectively evaluated. All patients were subjected to preoperative pulmonary artery catheterization; 17 were submitted to esophagogastric devascularization and splenectomy (EGDS) and 19 to distal splenorenal shunt (DSRS). The systemic hemodynamic assessment was repeated 4 d after the surgical procedure. RESULTS: Preoperative evaluation revealed (mean ± SD) an increased cardiac index (4.78 ± 1.13 L/min per m2),associated with a reduction in systemic vascular resistance index (1457 ± 380.7 dynes.s/cm5.m2). The mean pulmonary artery pressure (18 ± 5.1 mmHg) as well as the right atrial pressure (7.9 ± 2.5 mmHg) were increased,while the pulmonary vascular resistance index (133 ± 62 dynes.s/cm5.m2) was decreased. Four days after EGDS,a significant reduction in cardiac index (3.80 ± 0.4 L/min per m2,P < 0.001) and increase in systemic vascular resistance index (1901.4 ± 330.2 dynes.s/cm5. m2,P < 0.001) toward normal levels were observed. There was also a significant reduction in pulmonary artery pressure (12.65 ± 4.7 mmHg,P < 0.001) and no significant changes in the pulmonary vascular resistance index (141.6 ± 102.9 dynes.s/cm5.m2). Four days after DSRS,a non-significant increase in cardiac index (5.2 ± 0.76 L/min per m2) and systemic vascular resistance index (1389 ± 311 dynes.s/cm5.m2) was observed. There was also a non-significant increase in pulmonary artery pressure (19.84 ± 5.2 mmHg),right cardiac work index (1.38 ± 0.4 kg.m/m2) and right ventricular systolic work index (16.3 ± 6.3 g.m/m2),without significant changes in the pulmonary vascular resistance index (139.7 ± 67.8 dynes.s/cm5.m2). CONCLUSION: The hyperdynamic circulatory state observed in mansonic schistosomiasis was corrected by EGDS,but was maintained in patients who underwent DSRS. Similarly,the elevated mean pulmonary artery pressure was corrected after EGDS and maintained after DSRS. EGDS seems to be the most physiologic surgery for patients with schistosomal portal hypertension.展开更多
Schwannoma is a tumor derived from Schwann cells which usually arises in the upper extremities, trunk, head and neck, retroperitoneum, mediastinum, pelvis, and peritoneum. However, it can arise in the gastro-intestina...Schwannoma is a tumor derived from Schwann cells which usually arises in the upper extremities, trunk, head and neck, retroperitoneum, mediastinum, pelvis, and peritoneum. However, it can arise in the gastro-intestinal tract, including biliary tract. We present a 24-year-old male patient with obstructive jaundice, whose investigation with computed tomography abdomen showed focal wall thickening in the common hepatic duct, difficult to differentiate with hilar adenocarcinoma. He was diagnosed intraoperatively schwannoma of common bile duct and treated with local resection. The patient recovered well without signs of recurrence of the lesion after 12 mo. We also reviewed the common bile duct schwannoma related in the literature and evaluated the difficulty in pre and intraoperative differential diagnosis with adenocarcinoma hilar. Resection is the treatment of choice for such cases and the tumordid not recur in any of the resected cases.展开更多
AIM：To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics.METHODS： Methylation-specific PCR （MSP） assay was per...AIM：To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics.METHODS： Methylation-specific PCR （MSP） assay was performed in 13 nonneoplastic gastric adenocarcinorna, 30 intestinal-type gastric adenocarcinorna and 35 diffuse-type gastric adenocarcinorna samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher＇s exact test to assess associations between rnethylation status and clinico-pathological characteristics.RESULTS： Hypermethylation frequencies of CDH1, FHIT, MTAPand PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hyperrnethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hyperrnethylation frequency was significantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender.CONCLUSION： Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition.展开更多
Familial adenomatous polyposis (FAP) is an autosomal dominant inherited syndrome characterized by multiple adenomatous polyps (predisposing to colorectal cancer development) and numerous extracolonic manifestations. T...Familial adenomatous polyposis (FAP) is an autosomal dominant inherited syndrome characterized by multiple adenomatous polyps (predisposing to colorectal cancer development) and numerous extracolonic manifestations. The underlying genetic burden generates variable clinical features that may influence operative management. As a precancerous hereditary condition, the rationale of performing a prophylactic surgery is a mainstay of FAP management. The purpose of the present paper is to bring up many controversial aspects regarding surgical treatment for FAP, and to discuss the results and perspectives of the operative choices and approaches. Preferably, the decision-making process should not be limited to the conventional confrontation of pros and cons of ileorectal anastomosis or restorative proctocolectomy. A wide discussion with the patient may evaluate issues such as age, genotype, family history, sphincter function, the presence or risk of desmoid disease, potential complications of each procedure and chances of postoperative surveillance. Therefore, the definition of the best moment and the choice of appropriate procedure constitute an individual decision that must take into consideration patient’s preferences and full information about the complex nature of the disease. All these facts reinforce the idea that FAP patients should be managed by experienced surgeons working in specialized centers to achieve the best immediate and long-term results.展开更多
文摘Background: The restrictive lockdown measures that were introduced as a response to the COVID-19 pandemic imposed constraints that affected university students’ drinking habits and mental health. Methods: The current study evaluated the rate of binge drinking among university students as they returned to normal life post-lockdown and whether binge drinking affected their mental health and quality of life. A cross-sectional study included 1382 university students (18 - 24 years) of one private Southwestern university in Brazil. Alcohol Use Disorders Identification, the Beck Depression Inventory-II, and the World Health Organization Quality of Life Brief were used. Data were collected after the COVID-19 pandemic lockdown period (from October 2021 to May 2022). Results: 52.6% of the students reported binge drinking, and 35.6% reported symptoms of depression. Binge drinkers were 1.41 times more likely to experience depressive symptoms when compared with non-binge drinkers, and binge drinking was a risk factor for lower quality of life in all three domains (physical, psychological and environmental). Conclusion: Our study highlights an increased prevalence of binge drinking in university students after the pandemic lockdown. The rise in binge drinking rates was linked to depressive symptoms and worsening of quality of life.
文摘Climate change is a reality. The burning of fossil fuels from oil, natural gas and coal is responsible for much of the pollution and the increase in the planet’s average temperature, which has raised discussions on the subject, given the emergencies related to climate. An energy transition to clean and renewable sources is necessary and urgent, but it will not be quick. In this sense, increasing the efficiency of oil extraction from existing sources is crucial, to avoid waste and the drilling of new wells. The purpose of this work was to add diffusive and dispersive terms to the Buckley-Leverett equation in order to incorporate extra phenomena in the temporal evolution between the water-oil and oil-water transitions in the pipeline. For this, the modified Buckley-Leverett equation was discretized via essentially weighted non-oscillatory schemes, coupled with a three-stage Runge-Kutta and a fourth-order centered finite difference methods. Then, computational simulations were performed and the results showed that new features emerge in the transitions, when compared to classical simulations. For instance, the dispersive term inhibits the diffusive term, adding oscillations, which indicates that the absorption of the fluid by the porous medium occurs in a non-homogeneous manner. Therefore, based on research such as this, decisions can be made regarding the replacement of the porous medium or the insertion of new components to delay the replacement.
文摘The identification of natural, plant-derived compounds with pesticidal properties is crucial for developing environmentally sustainable alternatives to synthetic pesticides. In this study, four major lignans—dihydroclusin, cubebin, clusin, and yatein—were isolated from the crude extract of Piper cubeba fruit. Phytotoxicity assays revealed herbicidal activity against Agrostis stolonifera, with dihydroclusin and clusin exhibiting the highest efficacy, inhibiting seed germination by 50% and showing IC50 values of 2.9 µM and 45 µM, respectively, against Lemna paucicostata. Additionally, all compounds, except dihydroclusin, demonstrated fungicidal activity against the strawberry anthracnose pathogen Colletotrichum fragariae. Moreover, only dihydroclusin exhibited larvicidal activity against Aedes aegypti, causing 96% mortality of mosquito larvae at the 100-ppm concentration tested. These findings highlight the broad-spectrum bioactivity of Piper cubeba lignans, suggesting their potential as alternative agents of synthetic pesticides for managing agricultural pests.
文摘The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.
文摘The expression of miRNAs is associated with a variety of diseases, including neoplasms. In recent years, a large number of abnormally expressed miRNAs have been shown to be effective in understanding the oncogenesis, development, progression and prognosis of meningiomas. Furthermore, it is known that miRNAs act as oncogenes or tumor suppressors and that they regulate essential molecular pathways such as transcription factors involved in the pluripotency phenotype of stem cells. Therefore, the aim of this study was to analyze the expression of microRNAs miR-34a, miR-145 and miR-221 that regulate the pluripotency pathway of stem cells and correlate with tumor recurrence in grade I meningiomas. We used 30 samples, belonging to 15 patients who presented recurrences of grade I meningiomas. We observed low expression levels of miR-34a in the group of tumor recurrences when compared to control individuals and primary tumors, which may be associated with the tumor suppressor role of this miR. The miR-145 also showed decreased expression levels between the control group and the group of tumor recurrences. We also observed decreased expression levels in miR-145 between the control group and the primary tumors group. MiR-221 did not differ between the studied groups. MiR-34a and miR-145 microRNAs that regulate the stem cell pluripotency pathway are shown to be hypo expressed in tumor recurrences of grade I meningiomas and are shown to be good candidates for prognosis and recurrence biomarkers in meningiomas.
基金supported by FAPESP (So Paulo Research Foundation, Brazil)CAPES (Coordination for the Improvement of Higher Level, Brazil)
文摘Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity.In our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus formation.Calluses were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf sheath.The secondary embryogenesis was formed in yellow-globular callus.The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis.Glucose was more efficiency in the presence of light.Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.
文摘AIM: To evaluate the effects of protein deprivation on the myenteric plexus of the esophagus of weanling rats. METHODS: Pregnant female Wistar rats were divided into 2 groups: nourished (N),receiving normal diet,and undernourished (D),receiving a protein-deprived diet,which continued after birth. At twenty-one days of age,13 esophagi from each group were submitted to light microscopy and morphometrical analysis employing the NADH diaphorase,NADPH diaphorase and acetylcholinesterase techniques. Three other esophagi from each group were evaluated with transmission electron microscopy (TEM). RESULTS: In both the NADH- and the NADPH-reactive mounts,the neurons of the N mounts were more intensely stained,while in the D esophagi only the larger neurons were reactive. Many myenteric neurons of N were intensely reactive for AChE activity but only a few neurons of D exhibited these aspects. Ultrastructural analysis revealed that the granular reticulum of N showed large numbers of ribosomes aligned on the outer surface of its regularly arranged membrane while the ribosomes of D were disposed in clusters. The chromatin was more homogeneously scattered inside the neuron nucleus of N as well as the granular component of the nucleolus was evidently more developed in this group. Statistically significant differences between N and D groups were detected in the total estimated number of neurons stained by the NADPH technique. CONCLUSION: The morphological and quantitative data shows that feeding with protein-deprived diet in 21-d old rats induces a delay in the development of the myenteric neurons of the esophagus.
基金Supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico(CNPq)Fundac■o de Amparo á Pesquisa do Estado de S■o Paulo(FAPESP)
文摘AIM： To evaluate the potential of S-nitroso-N-acetylcysteine （SNAC） in inhibition of lipid peroxidation and the effect of oral SNAC administration in the prevention of nonalcoholic fatty liver disease （NAFLD） in an animal model.METHODS： NAFLD was induced in Wistar male rats by choline-deficient diet for 4 wk. SNAC-treated animals （n=6） （1.4 mg/kg/day of SNAC, orally） were compared to 2 control groups： one （n=6） received PBS solution and the other （n=6） received NAC solution （7 mg/kg/d）. Histological variables were semiquantitated with respect to macro and microvacuolar fat changes, its zonal distribution, foci of necrosis, portal and perivenular fibrosis, and inflammatory infiltrate with zonal distribution. LOOHs from samples of liver homogenates were quantified by HPLC. Nitrate levels in plasma of portal vein were assessed by chemiluminescence. Aqueous low-density lipoprotein （LDL） suspensions （200 pg protein/mL） were incubated with CuCl2 （300 μmol/L） in the absence and presence of SNAC （300 μmol/L） for 15 h at 37 ℃ Extent of LDL oxidation was assessed by fluorimetry. Linoleic acid （LA） （18.8 μmol/L） oxidation was induced by soybean lipoxygenase （SLO） （0.056 μmol/L） at 37 ℃ in the presence and absence of N-acetylcysteine （NAC） and SNAC （56 and 560 pmol/L） and monitored at 234 nm. RESULTS： Animals in the control group developed moderate macro and microvesicular fatty changes in periportal area. SNAC-treated animals displayed only discrete histological alterations with absence of fatty changes and did not develop liver steatosis. The absence of NAFLD in the SNAC-treated group was positively correlated with a decrease in the concentration of LOOH in liver homogenate, compared to the control group （0.7±0.2 nmol/mg vs 3.2±0.4 nmol/mg protein, respectively, P〈0.05）, while serum levels of aminotransferases were unaltered. The ability of SNAC in preventing lipid peroxidation was confirmed in in vitro experiments using LA and LDL as model substrates. CONCLUSION： Oral administration of SNAC prevents the onset of NAFLD in Wistar rats fed with cholinedeficient diet. This effect is correlated with the ability of SNAC to block the propagation of lipid peroxidation in vitro and in vitro.
文摘The chemical composition of marabou（Dichrostachys cinerea） wood and its treatment with acetic acid were investigated.Two different treatment approaches,direct acetosolv and combined acid prehydrolysis/acetosolv,were evaluated.The effects of acetic acid concentration（50%,70% and 90%） and temperature（normal boiling temperature and 121°C） on yield of solids,solubilization of lignin and hemicelluloses and recovery of cellulose were evaluated for both treatments.High solubilization of marabou components was observed in the direct acetosolv treatment at 121°C,especially at the highest acetic acid concentration,where around 84.8% of lignin and 78% of hemicelluloses were removed.When the material was subjected to acid prehydrolysis prior to acetosolv treatment,lignin solubilization was improved,especially at low acetic acid concentrations.Above 80% of the solubilized lignin was recovered from the liquors in the direct acetosolv treatment,but the recovery was lower in the combined treatment.Cellulose was well preserved in all the treatment schemes.
文摘The article contains a list of 25 structured technical sheets on fruits and pseudo-fruits that will serve as guidance in improving the quality of health of people, whether with chronic diseases or not. The technical sheets contain data related to the composition, active ingredients, interaction with medication, health conditions in which they may be useful in health care by various health professionals and references used to structure the material referring to each fruit or pseudo-fruit.
文摘During the production of nitrile rubber, significant amounts of nitrogen in the form of ammonium are generated in the wastewater. The discharge of this high-nitrogen wastewater can lead to serious environmental issues, including eutrophication, disruption of aquatic ecosystems, and groundwater contamination. To mitigate these impacts, this research explored the bioremediation capabilities of the macroalgae Ulva lactuca (Chlorophyta) for removing nitrogen from nitrile rubber production wastewater. The study employed single-phase and Michaelis-Menten decay models based on ammonium consumption, using various dilutions of wastewater to identify the optimal concentration for treatment. The physiological state of the macroalgae was monitored by measuring the photosynthetic capacity and specific growth rate during the experiments. In the presence of U. lactuca, ammonium concentrations decreased in all treatment groups, confirming that the ammonium kinetics conformed to both applied models. Our results show that U. lactuca effectively reduces ammonium concentrations, with an approximate removal rate of 0.020 µM·g−1·min−1 across different wastewater concentrations (70%, 80%, 90%, and 100%). Notably, the treatments with 70%, 80%, and 90% wastewater strength achieved about 67% reduction in ammonium, demonstrating the alga’s capacity to treat high-nitrogen wastewater. The photosynthetic performance of U. lactuca initially declined in control conditions but stabilized across all treatments, highlighting its adaptability. The kinetic analysis using the Michaelis-Menten model indicated a Vmax of 1342 μM·g−1·DMh−1, suggesting a robust capacity for ammonium uptake when fully saturated. Our study underscores the potential of Ulva lactuca as a cost-effective and efficient agent for wastewater bioremediation, particularly in settings with high nitrogen loads.
文摘This study investigated the effects of yerba mate consumption, a South American beverage, on cardiac remodeling in rats. For this purpose, 24 male Wistar rats were divided into Control Group (CG) which received filtered water and a standard diet, and Yerba Mate Group (YM), 6 g of Ilex paraguariensis in 100 ml water and the same diet, for 30 days. The YM group showed a reduction in final body weight and food consumption without altering weight gain. Regarding cardiac remodeling, the YM group exhibited a decrease in the right ventricle weight/final body weight ratio, suggesting cardiac atrophy, without affecting the atria and left ventricle. There was no change in cardiomyocyte area or nuclear fractal dimension in both groups. However, animals that consumed yerba mate showed increased collagen deposition and a smaller fractal dimension in the left ventricle. The consumption of yerba mate at room temperature for 30 days induced changes in cardiac remodeling, as evidenced by increased collagen deposition and alterations in fractal dimension in the left ventricle.
文摘Togo’s economy is heavily dependent on rainfed agriculture. Therefore, anomalies in precipitation can have a significant impact on crop yields, affecting food production and security. Thus, monitoring anomalous climate conditions in Togo through the combination of precipitation satellite-based data and Standard Precipitation Index (SPI) help anticipate the development of drought scenarios or excessive rainfall, allowing farmers to adjust their strategies and minimize losses. Continuous and adequate spatial monitoring of these climate anomalies provided by satellite-based products can be central to an effective early warning system (EWS) implementation in Togo. Precipitation satellite-based products have been presented invaluable tools for assessing droughts and , offering timely and comprehensive data that supports a wide range of applications. In this study, we applied the Integrated Multi-satellite Retrievals for GPM (IMERG) rainfall product, a unified satellite global precipitation product developed by NASA, to identify and characterize the severity of dry and wet climate events in Togo during the period from 2001 to 2019. The Standard Precipitation Index (SPI), as the main index recommended by the World Meteorological Organization to monitor drought wide world, was selected as the reference index to monitor dry and wet climate events across Togo regions. The results show two distinct major climate periods in Togo in the timeframe analyzed (2001-2019), one dominated by wet events from 2008 to 2010, and a second marked by severe and extreme dry events from 2013 to 2015;MERG rainfall and SPI combination were able to capture these events consistently.
文摘Obesity is a highly prevalent disease worldwide, associated with substantial economic and social costs. Besides the traditional treatment approach with low-calorie diet and regular physical exercise, some adjunct therapies have been investigated to assist body fat loss in obese patients. Carboxytherapy is an invasive intervention already studied for a range of conditions, from wound healing to the treatment of abdominal adiposity, due to its oxidative effect on adipocytes, in addition to contributing to improved skin elasticity. Thus, this study aimed to investigate the efficacy and safety of carboxytherapy in reducing abdominal circumference in patients hospitalized for obesity treatment. Fifty-eight adult inpatients with obesity underwent an individualized treatment (low-calorie diet and daily physical exercise for 40 - 90 days) associated with a mean of 11 sessions of carboxytherapy in the abdominal region and were assessed for body mass index (BMI), abdominal circumference (AC), waist to hip ratio (WHR) and percent body fat (%BF). After the intervention, significant reductions were observed in all studied variables (p < 0.001). The adherence rate was 53.67%, and absences were mainly related to the discomfort caused by the sessions. However, no important side effects were registered. Carboxytherapy was a safe intervention for obese patients, and when employed in combination with an inpatient program involving a low-calorie diet and regular exercise, contributed to the reduction of abdominal adiposity.
文摘Bariatric and metabolic surgeries have gained extensive popularity and trust due to their documented efficacy and safety in managing not only obesity but also associated comorbidities such as diabetes mellitus, hypertension, dyslipidemia, sleep apnea, and joint pain. Traditionally, bariatric surgeries have been categorized into hypoabsorptive, restrictive, or hybrid approaches. However, these classifications inadequately reflect the complex anatomical and physiological alterations associated with modern surgical methodologies. This paper explores the evolution of metabolic surgeries, emphasizing the integration of physiological concepts into classic procedures to provide more tailored and effective treatment options for obesity and its comorbidities. Finally, the proposal for a new classification based on current metabolic concepts will facilitate communication among patients, doctors, and healthcare professionals. Additionally, it will enable a more didactic and standardized approach to data collection for conducting studies and publications.
文摘AIM：To compare narrow-band imaging（NBI）without image magnification,and chromoendoscopy with Lugol＇s solution for detecting high-grade dysplasia and intramu-cosal esophageal squamous cell carcinoma（SCC）in patients with head and neck cancer.METHODS：This was a prospective observational study of 129 patients with primary head and neck tumors consecutively referred to the Gastrointestinal Endoscopy Unit of Hospital das Clínicas,Sao Paulo University Medical School,Brazil,between August 2006 and Feb-ruary 2007.Conventional examinations with NBI and Lugol chromoendoscopy were consecutively performed,and the discovered lesions were mapped,recorded and sent for biopsy.The results of the three methods were compared regarding sensitivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood value and negative likelihood value.RESULTS：Of the 129 patients,nine（7%）were diag-nosed with SCC,5 of which were in situ and 4 which were intramucosal.All carcinomas were detected through NBI and Lugol chromoendoscopy.Only 4 le-sions were diagnosed through conventional examination,all of which were larger than 10 mm.CONCLUSION：NBI technology with optical filters has high sensitivity and high negative predictive value for detecting superficial esophageal SCC,and produces results comparable to those obtained with 2.5%Lugol chromoendoscopy.
文摘AIM: To investigate the systemic hemodynamic effects of two surgical procedures largely employed for treatment of schistosomal portal hypertension. METHODS: Thirty-six patients undergoing elective surgical treatment of portal hypertension due to hepatosplenic mansonic schistosomiasis were prospectively evaluated. All patients were subjected to preoperative pulmonary artery catheterization; 17 were submitted to esophagogastric devascularization and splenectomy (EGDS) and 19 to distal splenorenal shunt (DSRS). The systemic hemodynamic assessment was repeated 4 d after the surgical procedure. RESULTS: Preoperative evaluation revealed (mean ± SD) an increased cardiac index (4.78 ± 1.13 L/min per m2),associated with a reduction in systemic vascular resistance index (1457 ± 380.7 dynes.s/cm5.m2). The mean pulmonary artery pressure (18 ± 5.1 mmHg) as well as the right atrial pressure (7.9 ± 2.5 mmHg) were increased,while the pulmonary vascular resistance index (133 ± 62 dynes.s/cm5.m2) was decreased. Four days after EGDS,a significant reduction in cardiac index (3.80 ± 0.4 L/min per m2,P < 0.001) and increase in systemic vascular resistance index (1901.4 ± 330.2 dynes.s/cm5. m2,P < 0.001) toward normal levels were observed. There was also a significant reduction in pulmonary artery pressure (12.65 ± 4.7 mmHg,P < 0.001) and no significant changes in the pulmonary vascular resistance index (141.6 ± 102.9 dynes.s/cm5.m2). Four days after DSRS,a non-significant increase in cardiac index (5.2 ± 0.76 L/min per m2) and systemic vascular resistance index (1389 ± 311 dynes.s/cm5.m2) was observed. There was also a non-significant increase in pulmonary artery pressure (19.84 ± 5.2 mmHg),right cardiac work index (1.38 ± 0.4 kg.m/m2) and right ventricular systolic work index (16.3 ± 6.3 g.m/m2),without significant changes in the pulmonary vascular resistance index (139.7 ± 67.8 dynes.s/cm5.m2). CONCLUSION: The hyperdynamic circulatory state observed in mansonic schistosomiasis was corrected by EGDS,but was maintained in patients who underwent DSRS. Similarly,the elevated mean pulmonary artery pressure was corrected after EGDS and maintained after DSRS. EGDS seems to be the most physiologic surgery for patients with schistosomal portal hypertension.
文摘Schwannoma is a tumor derived from Schwann cells which usually arises in the upper extremities, trunk, head and neck, retroperitoneum, mediastinum, pelvis, and peritoneum. However, it can arise in the gastro-intestinal tract, including biliary tract. We present a 24-year-old male patient with obstructive jaundice, whose investigation with computed tomography abdomen showed focal wall thickening in the common hepatic duct, difficult to differentiate with hilar adenocarcinoma. He was diagnosed intraoperatively schwannoma of common bile duct and treated with local resection. The patient recovered well without signs of recurrence of the lesion after 12 mo. We also reviewed the common bile duct schwannoma related in the literature and evaluated the difficulty in pre and intraoperative differential diagnosis with adenocarcinoma hilar. Resection is the treatment of choice for such cases and the tumordid not recur in any of the resected cases.
基金Supported by Fundao de Amparo à Pesquisa do Estado de So Paulo, Coordenao de Aperfeioamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico
文摘AIM：To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics.METHODS： Methylation-specific PCR （MSP） assay was performed in 13 nonneoplastic gastric adenocarcinorna, 30 intestinal-type gastric adenocarcinorna and 35 diffuse-type gastric adenocarcinorna samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher＇s exact test to assess associations between rnethylation status and clinico-pathological characteristics.RESULTS： Hypermethylation frequencies of CDH1, FHIT, MTAPand PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hyperrnethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hyperrnethylation frequency was significantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender.CONCLUSION： Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition.
文摘Familial adenomatous polyposis (FAP) is an autosomal dominant inherited syndrome characterized by multiple adenomatous polyps (predisposing to colorectal cancer development) and numerous extracolonic manifestations. The underlying genetic burden generates variable clinical features that may influence operative management. As a precancerous hereditary condition, the rationale of performing a prophylactic surgery is a mainstay of FAP management. The purpose of the present paper is to bring up many controversial aspects regarding surgical treatment for FAP, and to discuss the results and perspectives of the operative choices and approaches. Preferably, the decision-making process should not be limited to the conventional confrontation of pros and cons of ileorectal anastomosis or restorative proctocolectomy. A wide discussion with the patient may evaluate issues such as age, genotype, family history, sphincter function, the presence or risk of desmoid disease, potential complications of each procedure and chances of postoperative surveillance. Therefore, the definition of the best moment and the choice of appropriate procedure constitute an individual decision that must take into consideration patient’s preferences and full information about the complex nature of the disease. All these facts reinforce the idea that FAP patients should be managed by experienced surgeons working in specialized centers to achieve the best immediate and long-term results.
