Gout has an increasing morbidity rate around the world,correlating well with hypertension,hyperlipidemia,atherosclerosis,obesity and insulin resistance,and so on.In view of Traditional Chinese Medicine(TCM),gout belon...Gout has an increasing morbidity rate around the world,correlating well with hypertension,hyperlipidemia,atherosclerosis,obesity and insulin resistance,and so on.In view of Traditional Chinese Medicine(TCM),gout belongs to arthromyodynia.Keys to syndrome differentiation of TCM are in differentiation,corporeality and coated tongue(characterized to be damp).According to the dialectical characteristics of gout TCM,this paper summarized the research of gout TCM,including food-based traditional Chinese medicine,bill of lading medicine,drug extraction,optimal herbs and dosage,individual herb and compound prescription,in order to explore and elaborate the objective curative effect and exact mechanism of gout treated by TCM.展开更多
Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ov...Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ovariectomy(OVX)combined with chronic unpredictable mild stimulation(CUMS),54 female Kunming mice were randomly divided into Blank group,Model group(OVX+CUMS group),Sham operation group(SHAM group),Estradiol group(E2 group 20.090 mg∙Kg-1∙d-1),Naringin group(100 mg∙Kg-1∙d-1),Naringin+ICI182780 group(100 mg∙Kg-1∙d-1+0.075 mg∙Kg-1∙d-1),each group of 9 mice were administered for 21 consecutive days.The depression-like behaviour changes of each group of mice were observed by tail suspension and forced swimming experiments;The morphological changes of hippocampal neurons were observed by HE staining;Immunohistochemical method was used to detect the expression of ERαand ERβpositive cells in the hippocampus of mice;The expression of ERβ,GluR2,CAMK栻,NMDAR1,Bad,Bcl-2 and Caspase3 proteins in the hippocampus of the mice was detected by western blot.Results:Compared with the Blank group,the immobility time of the mice in the OVX+CUMS group was significantly increased(P<0.01),mice hippocampal neurons with a sparsely organized cell arrangement,nuclear fixation,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P<0.001),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.05,P<0.01);Compared with the OVX+CUMS group,the immobility time of the mice in the SHAM group,E2 group and Naringin group was significantly reduced(P<0.01),mice hippocampal neuronal cells are relatively intact and tightly packed,the expressions of ERβ,GluR2 and Bcl-2 were increased(P<0.01,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad and Caspase3 were decreased(P<0.05,P<0.01);Compared with the Naringin group,the quiescent time of the mice in the Naringin+ICI182780 group was increased(P<0.05),the smaller cell body of mouse hippocampal neurons,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P>0.05,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.01,P<0.05).Conclusions:Naringin may reduce neuronal cell excitotoxicity caused by glutamate receptor activation,reduce neuronal cell apoptosis and improve depression-like behavior by activating ERβreceptors in the hippocampus in a mouse model of menopause depression.展开更多
基金Supported by Traditional Chinese Medicine Research Project of Wuxi Commission of Health on"Study on the Mechanism of Bi Xie Yin on Neutrophils and Inflammatory Signal Transduction Pathway of NLRP3 in Gout Model Rats"(Project No.:ZYZL201803)Six Talent Peaks Project in Jiangsu Province on Clinical Application of huRAT1 Combined with ABCG2 Gene Detection in Predicting Hyperuricemia/Gout Susceptibility(Project No.:2015-WSN-089)
文摘Gout has an increasing morbidity rate around the world,correlating well with hypertension,hyperlipidemia,atherosclerosis,obesity and insulin resistance,and so on.In view of Traditional Chinese Medicine(TCM),gout belongs to arthromyodynia.Keys to syndrome differentiation of TCM are in differentiation,corporeality and coated tongue(characterized to be damp).According to the dialectical characteristics of gout TCM,this paper summarized the research of gout TCM,including food-based traditional Chinese medicine,bill of lading medicine,drug extraction,optimal herbs and dosage,individual herb and compound prescription,in order to explore and elaborate the objective curative effect and exact mechanism of gout treated by TCM.
基金Research Project of Wuxi Municipal Health Commission (No.M202206)。
文摘Objective:To explore the effect of naringin on hippocampus estrogen receptor and neuronal cell apoptosis in a mouse model of menopausal depression.Methods:The menopausal depression model was replicated by bilateral ovariectomy(OVX)combined with chronic unpredictable mild stimulation(CUMS),54 female Kunming mice were randomly divided into Blank group,Model group(OVX+CUMS group),Sham operation group(SHAM group),Estradiol group(E2 group 20.090 mg∙Kg-1∙d-1),Naringin group(100 mg∙Kg-1∙d-1),Naringin+ICI182780 group(100 mg∙Kg-1∙d-1+0.075 mg∙Kg-1∙d-1),each group of 9 mice were administered for 21 consecutive days.The depression-like behaviour changes of each group of mice were observed by tail suspension and forced swimming experiments;The morphological changes of hippocampal neurons were observed by HE staining;Immunohistochemical method was used to detect the expression of ERαand ERβpositive cells in the hippocampus of mice;The expression of ERβ,GluR2,CAMK栻,NMDAR1,Bad,Bcl-2 and Caspase3 proteins in the hippocampus of the mice was detected by western blot.Results:Compared with the Blank group,the immobility time of the mice in the OVX+CUMS group was significantly increased(P<0.01),mice hippocampal neurons with a sparsely organized cell arrangement,nuclear fixation,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P<0.001),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.05,P<0.01);Compared with the OVX+CUMS group,the immobility time of the mice in the SHAM group,E2 group and Naringin group was significantly reduced(P<0.01),mice hippocampal neuronal cells are relatively intact and tightly packed,the expressions of ERβ,GluR2 and Bcl-2 were increased(P<0.01,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad and Caspase3 were decreased(P<0.05,P<0.01);Compared with the Naringin group,the quiescent time of the mice in the Naringin+ICI182780 group was increased(P<0.05),the smaller cell body of mouse hippocampal neurons,the expressions of ERβ,GluR2,and Bcl-2 were decreased(P<0.01,P>0.05,P<0.05),and the expressions of NMDAR1,CAMK栻,Bad,and Caspase3 were increased(P<0.01,P<0.05).Conclusions:Naringin may reduce neuronal cell excitotoxicity caused by glutamate receptor activation,reduce neuronal cell apoptosis and improve depression-like behavior by activating ERβreceptors in the hippocampus in a mouse model of menopause depression.
