A field experiment was conducted at the farm of Yangzhou University, Yangzhou, China, to study the effects of organic fertilizers made from maize straw on rice grain yield and the emission of greenhouse gases. Four or...A field experiment was conducted at the farm of Yangzhou University, Yangzhou, China, to study the effects of organic fertilizers made from maize straw on rice grain yield and the emission of greenhouse gases. Four organic fertilizer treatments were as follows： maize straw （MS）, compost made from maize straw （MC）, methane-generating maize residue （MR）, and black carbon made from maize straw （BC）. These organic fertilizers were applied separately to paddy fields before rice transplanting. No organic fertilizer was applied to the control （CK）. The effects of each organic fertilizer on rice grain yield and emission of greenhouse gases were investigated under two conditions, namely, no nitrogen （N） application （ON） and site-specific N management （SSNM）. Rice grain yields were significantly higher in the MS, MC and MR treatments than those in CK under either ON or SSNM. The MS treatment resulted in the highest grain yield and agronomic N use efficiency. However, no significant difference was observed for these parameters between the BC treatment and CK. The changes in the emissions of methane （CH4） carbon dioxide （CO2）, or nitrous oxide （N20） from the fields were similar among all organic fertilizer treatments during the entire rice growing season. The application of each organic fertilizer significantly increased the emission of each greenhouse gas （except N20 emission in the BC treatment） and global warming potential （GWP）. Emissions of all the greenhouse gases and GWP increased under the same organic fertilizer treatment in the presence of N fertilizer, whereas GWP per unit grain yield decreased. The results indicate that the application of organic fertilizer （MS, MC or MR） could increase grain yield, but also could enhance the emissions of greenhouse gases from paddy fields. High grain yield and environmental efficiency could be achieved by applying SSNM with MR.展开更多
In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribb...In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.展开更多
Real-time monitoring and investigation in fields showed that the disease starting period of Sclerotinia sclerotiorum in mechanical direct seeding fields was earlier. Disease conditions rose with the advancing growth s...Real-time monitoring and investigation in fields showed that the disease starting period of Sclerotinia sclerotiorum in mechanical direct seeding fields was earlier. Disease conditions rose with the advancing growth stage, which grew more quickly during the early stage of anthesis, flower withering period and early si- lique mature period. Yield less rate increased with the increasing stem disease rate and disease index. Yield loss rate（ Y1 ） had extremely significant correlation with stem disease rate（X1 ）and disease index（X2 ）, and the related models were Y1 = 0. 544 330 2X1 -2. 316 1 （n = 14, r --0. 986 0 ＊ ＊ ）and YI = 0. 673 408 X2 - 1. 016 4 （ n = 14 ,r = 0.977 2 ~ ＊ ）, respectively. Disease control indexes for one time control against S. sclerotlorum in mechanical direct seeding fields were stem disease rate as 11.8% and disease index as 7.6, respectively. The control indexes for twice control against the disease were stem disease rate as 16.8% and disease index as 11.7. This paper provided a new scientific basis for preventing S. sclerotiorum in mechanical direct seeding fields.展开更多
[ Objective ] This study aimed to explore immature embryo culture of Lagerstroemia indica and investigate the appropriate conditions for growth and differentiation. [ Method] Immature embryos of L. indica were employe...[ Objective ] This study aimed to explore immature embryo culture of Lagerstroemia indica and investigate the appropriate conditions for growth and differentiation. [ Method] Immature embryos of L. indica were employed as the explants for germination induction to establish aseptic lines. Based on that, the effects of different hormone levels and culture conditions on immature embryo culture of L. indica were analyzed. [ Result ] Peeled immature embryos of L. indica were germinated easily, leading to a germination rate of 100%. The optimal initial medium was MS ＋ BA0.5 ＋ NAA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ; the optimal shoot induction medium was MS ＋ BA0.5 ＋ NAA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ＋ coconut milk 10% ; the optimal rooting medium was MS ＋ BA0.5 ＋ IBA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ＋ coconut milk 10%. [ Conclusion] This study provided a technical reference for subsequent optimized breeding of L. indica.展开更多
[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive cont...[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).S.exigua and S.litura occurred severely at seedling stage;S.litura,S.exigua and P.xylostella occurred simultaneously at rosette and bud growth period;P.xylostella damaged florets of lateral branches in late harvest period.The trapping effects of combination A and E against S.litura,S.exigua and P.xylostella were the best;more than 1260 individuals of S.litura,S.exigua and P.xylostella were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically controlled.With the sex attract produced in Ningbo as an example,the combination of S.litura S type∶S.exigua S type∶P.xylostella blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for P.xylostella),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on S.litura,S.exigua and P.xylostella were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.展开更多
The field experiment results showed that 250 g/l mandipropamid suspension had good control effect on red taro blight,and the 1∶1500 suspension was applied twice continuously during the early period( June 25) and culm...The field experiment results showed that 250 g/l mandipropamid suspension had good control effect on red taro blight,and the 1∶1500 suspension was applied twice continuously during the early period( June 25) and culmination period( July 12) of taro blight; the control effect 23 d after the last application reached 79. 28%,significantly better than that of conventional pesticide,and it was safe,so it had good prospects for the development and application in production.展开更多
[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Metho...[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Method] The monitoring data of sexual attractants at fixed points for 11 consecutive years were summarized and analyzed.[Result] The large occurrence frequency of S.litura in Taizhou was 36.4%;the annual movement showed a downward-opening parabolic form,and the movement trajectory was N =-18.416 t^(2)+182.51 t +179.71,t ={1,2,3,…,n}(n =11;r =0.691 7*).The seasonal fluctuation showed a trend of peak oscillation attenuation curve,and the total moth quantity gradually decreased from sowing to heading stage.The functional model of average daily quantity was m=0.000 2 d^(2)-0.067 5 d+6.797 3,d={1,2,3,…,n}(n =186,r=0.955 2**).Air temperature was the main external factor affecting the seasonal variation of quantity,and there was a significant correlation be-tween the number of ten-day moth trapping quantity and ten-day average temperature:M =0.243 T^(2)-6.935 1 T +46.681(n =108,r =0.560 2**)or M=2.734 4 T-35.446(n=108,r=0.500 3**).Therefore,the moth quantity in the field increased with the increase of ten-day average temperature,and the period from July to September with high temperature and less rainfall was the peak occurrence period of S.litura.[Conclusion] These models can be used to improve the prediction and early warning levels of S.litura in broccoli,and has good guiding significance for decision-making and prevention.展开更多
[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to ...[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to 2018.[Result]The use of carbendazim or carbendazim+mancozeb,and carbendazim+embamycin had significant control effect on stem base rot of sweet potato.However,the applied concentration should be higher than the recommended concentration.Especially the concentration of 50%carbendazim WP should be no more than 300 times,and the prevention and control effect of 100 times dilution would be better.[Conclusion]The types and concentrations of prevention and control agents are determined in the test,which will provide certain basis for chemical prevention and control against stem base rot of sweet potato.展开更多
Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance（ST） was genetically dissected by phenotypin...Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance（ST） was genetically dissected by phenotyping two sets of BC2F5 introgression lines（ILs） for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL（AB-QTL） analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and nonstress conditions were identified, most（〉90%） of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan（HHZ） ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection（MAS） or genome selection.展开更多
基金supported by the grants from the National Basic Research Program(Grant No.2009CB118603)the National Science Foundation of China(NSFC-IRRI Joint Research Project,Grant No.31061140457)+3 种基金the Natural Science Foundation of China(Grant Nos.31071360 and 31271641)the Basic Scientific Research Special Operation Cost of the Central Research Institutions(Grant Nos.201103003 and 201203079)the National Key Technology Support Program of China(Grant Nos.2011BAD16B14 and 2012BAD04B08)the Jiangsu Advantages of Key Construction Projects and Research Innovation Project by Graduate Student(Grant No.CXZZ13_0902)
文摘A field experiment was conducted at the farm of Yangzhou University, Yangzhou, China, to study the effects of organic fertilizers made from maize straw on rice grain yield and the emission of greenhouse gases. Four organic fertilizer treatments were as follows： maize straw （MS）, compost made from maize straw （MC）, methane-generating maize residue （MR）, and black carbon made from maize straw （BC）. These organic fertilizers were applied separately to paddy fields before rice transplanting. No organic fertilizer was applied to the control （CK）. The effects of each organic fertilizer on rice grain yield and emission of greenhouse gases were investigated under two conditions, namely, no nitrogen （N） application （ON） and site-specific N management （SSNM）. Rice grain yields were significantly higher in the MS, MC and MR treatments than those in CK under either ON or SSNM. The MS treatment resulted in the highest grain yield and agronomic N use efficiency. However, no significant difference was observed for these parameters between the BC treatment and CK. The changes in the emissions of methane （CH4） carbon dioxide （CO2）, or nitrous oxide （N20） from the fields were similar among all organic fertilizer treatments during the entire rice growing season. The application of each organic fertilizer significantly increased the emission of each greenhouse gas （except N20 emission in the BC treatment） and global warming potential （GWP）. Emissions of all the greenhouse gases and GWP increased under the same organic fertilizer treatment in the presence of N fertilizer, whereas GWP per unit grain yield decreased. The results indicate that the application of organic fertilizer （MS, MC or MR） could increase grain yield, but also could enhance the emissions of greenhouse gases from paddy fields. High grain yield and environmental efficiency could be achieved by applying SSNM with MR.
基金Supported by"San Nong Liu Fang"Science and Technology Cooperation Project of Zhejiang Province(ZNJF[2011]No.85)Major Project of Science and Technology of Zhejiang Province(2009C2006-1-8)
文摘In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.
基金Supported by Agricultural Key Projects of Science and Technology Plan of Taizhou City(102KY03)
文摘Real-time monitoring and investigation in fields showed that the disease starting period of Sclerotinia sclerotiorum in mechanical direct seeding fields was earlier. Disease conditions rose with the advancing growth stage, which grew more quickly during the early stage of anthesis, flower withering period and early si- lique mature period. Yield less rate increased with the increasing stem disease rate and disease index. Yield loss rate（ Y1 ） had extremely significant correlation with stem disease rate（X1 ）and disease index（X2 ）, and the related models were Y1 = 0. 544 330 2X1 -2. 316 1 （n = 14, r --0. 986 0 ＊ ＊ ）and YI = 0. 673 408 X2 - 1. 016 4 （ n = 14 ,r = 0.977 2 ~ ＊ ）, respectively. Disease control indexes for one time control against S. sclerotlorum in mechanical direct seeding fields were stem disease rate as 11.8% and disease index as 7.6, respectively. The control indexes for twice control against the disease were stem disease rate as 16.8% and disease index as 11.7. This paper provided a new scientific basis for preventing S. sclerotiorum in mechanical direct seeding fields.
文摘[ Objective ] This study aimed to explore immature embryo culture of Lagerstroemia indica and investigate the appropriate conditions for growth and differentiation. [ Method] Immature embryos of L. indica were employed as the explants for germination induction to establish aseptic lines. Based on that, the effects of different hormone levels and culture conditions on immature embryo culture of L. indica were analyzed. [ Result ] Peeled immature embryos of L. indica were germinated easily, leading to a germination rate of 100%. The optimal initial medium was MS ＋ BA0.5 ＋ NAA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ; the optimal shoot induction medium was MS ＋ BA0.5 ＋ NAA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ＋ coconut milk 10% ; the optimal rooting medium was MS ＋ BA0.5 ＋ IBA0.1 ＋ sucrose 3.0% ＋ agar 0.7% ＋ coconut milk 10%. [ Conclusion] This study provided a technical reference for subsequent optimized breeding of L. indica.
基金Supported by Science and Technology Project of Taizhou City"Research,Integration and Industrial-ization Development of Broccoli Production Technology Coping with Japan’s Affirmative List System(Research and Application of Key Technologies in Promoting Broccoli Industry)"(071TG04)Taizhou Broccoli Breeding and Promotion Integration Innovation Team Project(TRCL[2014]No.7)。
文摘[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).S.exigua and S.litura occurred severely at seedling stage;S.litura,S.exigua and P.xylostella occurred simultaneously at rosette and bud growth period;P.xylostella damaged florets of lateral branches in late harvest period.The trapping effects of combination A and E against S.litura,S.exigua and P.xylostella were the best;more than 1260 individuals of S.litura,S.exigua and P.xylostella were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically controlled.With the sex attract produced in Ningbo as an example,the combination of S.litura S type∶S.exigua S type∶P.xylostella blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for P.xylostella),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on S.litura,S.exigua and P.xylostella were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.
基金Supported by Taizhou Science and Technology Plan Project(Agriculture)(131-ky24/16ny05)
文摘The field experiment results showed that 250 g/l mandipropamid suspension had good control effect on red taro blight,and the 1∶1500 suspension was applied twice continuously during the early period( June 25) and culmination period( July 12) of taro blight; the control effect 23 d after the last application reached 79. 28%,significantly better than that of conventional pesticide,and it was safe,so it had good prospects for the development and application in production.
基金Supported by Science and Technology Planning Project of Taizhou City (071TG04)Taizhou Broccoli Breeding,Propagation and Promotion Integrated Innovation Team Project (TRCL[2014]7)。
文摘[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Method] The monitoring data of sexual attractants at fixed points for 11 consecutive years were summarized and analyzed.[Result] The large occurrence frequency of S.litura in Taizhou was 36.4%;the annual movement showed a downward-opening parabolic form,and the movement trajectory was N =-18.416 t^(2)+182.51 t +179.71,t ={1,2,3,…,n}(n =11;r =0.691 7*).The seasonal fluctuation showed a trend of peak oscillation attenuation curve,and the total moth quantity gradually decreased from sowing to heading stage.The functional model of average daily quantity was m=0.000 2 d^(2)-0.067 5 d+6.797 3,d={1,2,3,…,n}(n =186,r=0.955 2**).Air temperature was the main external factor affecting the seasonal variation of quantity,and there was a significant correlation be-tween the number of ten-day moth trapping quantity and ten-day average temperature:M =0.243 T^(2)-6.935 1 T +46.681(n =108,r =0.560 2**)or M=2.734 4 T-35.446(n=108,r=0.500 3**).Therefore,the moth quantity in the field increased with the increase of ten-day average temperature,and the period from July to September with high temperature and less rainfall was the peak occurrence period of S.litura.[Conclusion] These models can be used to improve the prediction and early warning levels of S.litura in broccoli,and has good guiding significance for decision-making and prevention.
基金Major Science and Technology Project for New Variety Breeding of Agriculture(Grain)in Zhejiang Province"Sweet Potato,Yam Germplasm Screening and New Variety Breeding"(2016C 02050-7-3)Key Agricultural Science and Technology Project of Taizhou City"Study on Pathogen Identification,Occurrence Regularity and Prevention and Control Technology of Sweet Potato Stem Base Rot(1701ny12)+2 种基金China Agricultural(Sweet Potato)Industry Research System“Fresh Sweet Potato Improvement"(CARS-10-C-17)Project of Guangzhou Comprehensive Test Station(CARS-10-B-4)2017 Special Project on Agricultural Development and Rural Work in Guangdong Province"Creation and Application of New Sweet Potato Varieties for High Quality and Resistance".
文摘[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to 2018.[Result]The use of carbendazim or carbendazim+mancozeb,and carbendazim+embamycin had significant control effect on stem base rot of sweet potato.However,the applied concentration should be higher than the recommended concentration.Especially the concentration of 50%carbendazim WP should be no more than 300 times,and the prevention and control effect of 100 times dilution would be better.[Conclusion]The types and concentrations of prevention and control agents are determined in the test,which will provide certain basis for chemical prevention and control against stem base rot of sweet potato.
基金funded by the National High-Tech R&D Program of China (2012AA101101)the 948 Project from the Ministry of Agriculture, China (2010-G2B)+1 种基金the International Cooperative Project from the Ministry of Science and Technology, China (S2012ZR0160)the Bill & Melinda Gates Foundation Project (OPP51587)
文摘Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance（ST） was genetically dissected by phenotyping two sets of BC2F5 introgression lines（ILs） for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL（AB-QTL） analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and nonstress conditions were identified, most（〉90%） of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan（HHZ） ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection（MAS） or genome selection.
