Plant biofertilization involves introducing compounds containing living mi-croorganisms into the coating medium to sustainably enhance plant production and soil health. This is a complex process that undergoes multipl...Plant biofertilization involves introducing compounds containing living mi-croorganisms into the coating medium to sustainably enhance plant production and soil health. This is a complex process that undergoes multiple stages of development before yielding a final product. The final biofertilizer is used by legumes-protein-rich crops in symbiosis with rhizobia to enable biological nitrogen fixation increasing natural soil fertility. This study aims to determine the optimal formulation of a rhizobial biofertilizer to improve the performance of soybean (Glycine max L. cv. Docko). To this end, soybean seeds obtained from IRAD were coated with different formulations derived from locally sourced materials. Palm kernel oil was used as an adhesive in one group, while corn powder served as an adhesive in another. The coated seeds were then sown in the field. The results indicate that the combination of pigeon pea powder + sugarcane molasses, with palm kernel oil as an adhesive, produced the best nodulation (nitrogen fixation). This formulation also led to significant improvements in growth (+350%) and total nitrogen content (+1100%) compared to the bacterial broth inoculum control (B0) (P ≤ 0.01). These findings represent a significant advancement in improving nitrogen-fixing bacterial inoculants and enhancing soil fertility for the sustainable cultivation of soybeans in this tropical soil.展开更多
The aim of this work is to assess soil types’ effect on the growth and production of <em>Curcuma longa</em> rhizomes. The Rhizome of <em>Curcuma longa</em> was grown in a greenhouse in pots fo...The aim of this work is to assess soil types’ effect on the growth and production of <em>Curcuma longa</em> rhizomes. The Rhizome of <em>Curcuma longa</em> was grown in a greenhouse in pots for seven months on different soil types. Physico-chemical analyses of the different soils were carried out. Collar diameter, the height of the plants, and yield of rhizome were measured. Total microbial density, number of spores, and root colonization of arbuscular mycorrhizal fungi (AMF) from these soils were assessed. Results show that soils are sandy clay loam, clay sandy, clay, acidic (pH 4.16 to 6.62), and have a C/N ratio from 6.10 to 19.83. Nitrogen (N) is between 0.49 to 2.41 g/kg, available phosphorus (P) between 2 to 16 ppm, Organic matter (OM) from 14.6 to 51.4 g/kg. Total microbial density is between 2 to 16 × 10<sup>6</sup> Cell/mm<sup>3</sup>, number of AMF spores <em>in situ</em> between 4 to 67 spores/g of soil. The frequency of root colonization is between 47% to 78%. The average growth and rhizome production are between 5 to 15 g/plant. All the results obtained show that the soils with clay to sandy clayey loam texture, medium acidity (pH 5.5 - 6.6), low C content (7.3 - 9.6 g/kg), low N content (0.49 - 1.13 g/kg), good C/N ratio (<14), low to medium AMF sporulation (28 - 41 spores/g) and AMF root colonization between 55% to 78% are the most suitable for the cultivation and rhizome production of <em>C</em><em>urcuma longa</em> in Cameroon.展开更多
The aim of this research was to assess the diversity of the Cameroon cotton zone in soybean associated rhizobia in order to formulate the most efficient elite inoculant to boost both the cotton and soybean production....The aim of this research was to assess the diversity of the Cameroon cotton zone in soybean associated rhizobia in order to formulate the most efficient elite inoculant to boost both the cotton and soybean production. Therefore, soybean associated rhizobia were isolated and characterized morphologically, physiologically and biochemically on YEMA culture media. For each of the two soybean varieties (Houla1 and TGX1910 14F) used, the trials were laid out in two IRAD-fields of North Cameroon (Sanguere-Paul) and Far-North (Soukoundou) respectively, under a complete randomized complete block design, the isolate formulations representing the treatments. The six isolated strains (IS1, IS2, IS3, IS4, IS5, IS6) from which seven liquid inoculant were formulated were revealed to belong to the same slow growing group of rhizobia, with a high level of tolerance to temperature, pH, and salinity, with optimum growth at respectively 28˚C, pH (7 - 9), salt (1% - 5%). Not surprisingly, root nodules were formed by both inoculated and uninoculated soybean plants. However, the most efficient soybean-rhizobia symbiosis for nodulations were isolate IS6 associated to TGX1910 14F variety, and isolate IS5 associated to Houla1variety at Sanguere-Paul. Whereas isolate M was associated to TGX1910 14F variety, Houla 1 variety had affinity with native rhizobia isolates at Soukoundou. The present results suggest the adaptability of rhizobia isolates to a particular soybean variety at a particular cotton fields zone. These findings should be taken into consideration for commercial inoculant formulation.展开更多
文摘Plant biofertilization involves introducing compounds containing living mi-croorganisms into the coating medium to sustainably enhance plant production and soil health. This is a complex process that undergoes multiple stages of development before yielding a final product. The final biofertilizer is used by legumes-protein-rich crops in symbiosis with rhizobia to enable biological nitrogen fixation increasing natural soil fertility. This study aims to determine the optimal formulation of a rhizobial biofertilizer to improve the performance of soybean (Glycine max L. cv. Docko). To this end, soybean seeds obtained from IRAD were coated with different formulations derived from locally sourced materials. Palm kernel oil was used as an adhesive in one group, while corn powder served as an adhesive in another. The coated seeds were then sown in the field. The results indicate that the combination of pigeon pea powder + sugarcane molasses, with palm kernel oil as an adhesive, produced the best nodulation (nitrogen fixation). This formulation also led to significant improvements in growth (+350%) and total nitrogen content (+1100%) compared to the bacterial broth inoculum control (B0) (P ≤ 0.01). These findings represent a significant advancement in improving nitrogen-fixing bacterial inoculants and enhancing soil fertility for the sustainable cultivation of soybeans in this tropical soil.
文摘The aim of this work is to assess soil types’ effect on the growth and production of <em>Curcuma longa</em> rhizomes. The Rhizome of <em>Curcuma longa</em> was grown in a greenhouse in pots for seven months on different soil types. Physico-chemical analyses of the different soils were carried out. Collar diameter, the height of the plants, and yield of rhizome were measured. Total microbial density, number of spores, and root colonization of arbuscular mycorrhizal fungi (AMF) from these soils were assessed. Results show that soils are sandy clay loam, clay sandy, clay, acidic (pH 4.16 to 6.62), and have a C/N ratio from 6.10 to 19.83. Nitrogen (N) is between 0.49 to 2.41 g/kg, available phosphorus (P) between 2 to 16 ppm, Organic matter (OM) from 14.6 to 51.4 g/kg. Total microbial density is between 2 to 16 × 10<sup>6</sup> Cell/mm<sup>3</sup>, number of AMF spores <em>in situ</em> between 4 to 67 spores/g of soil. The frequency of root colonization is between 47% to 78%. The average growth and rhizome production are between 5 to 15 g/plant. All the results obtained show that the soils with clay to sandy clayey loam texture, medium acidity (pH 5.5 - 6.6), low C content (7.3 - 9.6 g/kg), low N content (0.49 - 1.13 g/kg), good C/N ratio (<14), low to medium AMF sporulation (28 - 41 spores/g) and AMF root colonization between 55% to 78% are the most suitable for the cultivation and rhizome production of <em>C</em><em>urcuma longa</em> in Cameroon.
文摘The aim of this research was to assess the diversity of the Cameroon cotton zone in soybean associated rhizobia in order to formulate the most efficient elite inoculant to boost both the cotton and soybean production. Therefore, soybean associated rhizobia were isolated and characterized morphologically, physiologically and biochemically on YEMA culture media. For each of the two soybean varieties (Houla1 and TGX1910 14F) used, the trials were laid out in two IRAD-fields of North Cameroon (Sanguere-Paul) and Far-North (Soukoundou) respectively, under a complete randomized complete block design, the isolate formulations representing the treatments. The six isolated strains (IS1, IS2, IS3, IS4, IS5, IS6) from which seven liquid inoculant were formulated were revealed to belong to the same slow growing group of rhizobia, with a high level of tolerance to temperature, pH, and salinity, with optimum growth at respectively 28˚C, pH (7 - 9), salt (1% - 5%). Not surprisingly, root nodules were formed by both inoculated and uninoculated soybean plants. However, the most efficient soybean-rhizobia symbiosis for nodulations were isolate IS6 associated to TGX1910 14F variety, and isolate IS5 associated to Houla1variety at Sanguere-Paul. Whereas isolate M was associated to TGX1910 14F variety, Houla 1 variety had affinity with native rhizobia isolates at Soukoundou. The present results suggest the adaptability of rhizobia isolates to a particular soybean variety at a particular cotton fields zone. These findings should be taken into consideration for commercial inoculant formulation.
