Objective:To analyze the factors related to vessel vasovagal reaction(VVR)in apheresis donors,establish a mathematical model for predicting the correlation factors and occurrence risk,and use the prediction model to i...Objective:To analyze the factors related to vessel vasovagal reaction(VVR)in apheresis donors,establish a mathematical model for predicting the correlation factors and occurrence risk,and use the prediction model to intervene in high-risk VVR blood donors,improve the blood donation experience,and retain blood donors.Methods:A total of 316 blood donors from the Xi'an Central Blood Bank from June to September 2022 were selected to statistically analyze VVR-related factors.A BP neural network prediction model is established with relevant factors as input and DRVR risk as output.Results:First-time blood donors had a high risk of VVR,female risk was high,and sex difference was significant(P value<0.05).The blood pressure before donation and intergroup differences were also significant(P value<0.05).After training,the established BP neural network model has a minimum RMS error of o.116,a correlation coefficient R=0.75,and a test model accuracy of 66.7%.Conclusion:First-time blood donors,women,and relatively low blood pressure are all high-risk groups for VVR.The BP neural network prediction model established in this paper has certain prediction accuracy and can be used as a means to evaluate the risk degree of clinical blood donors.展开更多
目的探讨血小板储存过程中伴随储存时间延长pri-mioroRNA-223的表达变化及意义。方法使用血细胞分析仪检测不同保存时间(1、3、5 d)的血小板质量,并进行代谢组学检测。同时,建立液滴式数字PCR(droplet digital PCR,ddPCR)方法,对其敏感...目的探讨血小板储存过程中伴随储存时间延长pri-mioroRNA-223的表达变化及意义。方法使用血细胞分析仪检测不同保存时间(1、3、5 d)的血小板质量,并进行代谢组学检测。同时,建立液滴式数字PCR(droplet digital PCR,ddPCR)方法,对其敏感性、重复性进行评价。用ddPCR检测血小板样本不同储存时间的pri-miR-223表达量,分析其对血小板储存损伤(PSL)的影响及意义。结果随着保存时间的延长,血小板不同保存时间的血小板计数(PLT)、血小板比例(P-LCR)、平均血小板体积(MPV)和血小板分布宽度(PDW)差异均无统计学意义(P>0.05)。而代谢组学研究共筛选出955种代谢产物,其中54种代谢产物上调,6种代谢产物下调。所建立的ddPCR方法特异性好,敏感性高,标准曲线表现出良好的线性相关性(Y=0.9736X+0.0832),R^(2)值为0.9984,线性动态范围灵敏度为3.3×10~0~1.9×10~5拷贝/μL。ddPCR结果显示第3、5天样本中pri-miR-223表达量比第1天明显降低。结论随着血小板体外贮存时间的延长,对PLT、P-LCR、MPV和PDW没有影响,储存期末部分代谢产物改变,且通过ddPCR检测发现pri-miR-223表达量降低,这种代谢变化可能与pri-miR-223的表达调控有关。展开更多
基金Xi'an Municipal Bureau of Science and Technology,Science and Technology Program,Medical Research Project。
文摘Objective:To analyze the factors related to vessel vasovagal reaction(VVR)in apheresis donors,establish a mathematical model for predicting the correlation factors and occurrence risk,and use the prediction model to intervene in high-risk VVR blood donors,improve the blood donation experience,and retain blood donors.Methods:A total of 316 blood donors from the Xi'an Central Blood Bank from June to September 2022 were selected to statistically analyze VVR-related factors.A BP neural network prediction model is established with relevant factors as input and DRVR risk as output.Results:First-time blood donors had a high risk of VVR,female risk was high,and sex difference was significant(P value<0.05).The blood pressure before donation and intergroup differences were also significant(P value<0.05).After training,the established BP neural network model has a minimum RMS error of o.116,a correlation coefficient R=0.75,and a test model accuracy of 66.7%.Conclusion:First-time blood donors,women,and relatively low blood pressure are all high-risk groups for VVR.The BP neural network prediction model established in this paper has certain prediction accuracy and can be used as a means to evaluate the risk degree of clinical blood donors.
文摘目的探讨血小板储存过程中伴随储存时间延长pri-mioroRNA-223的表达变化及意义。方法使用血细胞分析仪检测不同保存时间(1、3、5 d)的血小板质量,并进行代谢组学检测。同时,建立液滴式数字PCR(droplet digital PCR,ddPCR)方法,对其敏感性、重复性进行评价。用ddPCR检测血小板样本不同储存时间的pri-miR-223表达量,分析其对血小板储存损伤(PSL)的影响及意义。结果随着保存时间的延长,血小板不同保存时间的血小板计数(PLT)、血小板比例(P-LCR)、平均血小板体积(MPV)和血小板分布宽度(PDW)差异均无统计学意义(P>0.05)。而代谢组学研究共筛选出955种代谢产物,其中54种代谢产物上调,6种代谢产物下调。所建立的ddPCR方法特异性好,敏感性高,标准曲线表现出良好的线性相关性(Y=0.9736X+0.0832),R^(2)值为0.9984,线性动态范围灵敏度为3.3×10~0~1.9×10~5拷贝/μL。ddPCR结果显示第3、5天样本中pri-miR-223表达量比第1天明显降低。结论随着血小板体外贮存时间的延长,对PLT、P-LCR、MPV和PDW没有影响,储存期末部分代谢产物改变,且通过ddPCR检测发现pri-miR-223表达量降低,这种代谢变化可能与pri-miR-223的表达调控有关。
