Escherichia coli type 1 was used as a model system to determine whether static magnetic fields are a general stress factor. The bacterial broth culture were exposed to different magnetic force （400, 800, 1200 and 160...Escherichia coli type 1 was used as a model system to determine whether static magnetic fields are a general stress factor. The bacterial broth culture were exposed to different magnetic force （400, 800, 1200 and 1600 Gauss） with incubation at 37 ℃ for different times （24, 48 and 72 hrs） under aerobic conditions. The response of the cells to the magnetic fields was estimated from the change in total protein synthesis by using spectrophotometer at 550 nm and by using of SDS-PAGE （Sodium dodecyl sulfate- polyacrylamide gel electrophoresis）. Results concluded that was approximately no reproducible changes qualitatively in extracellular proteins were observed in the SDS-PAGE electrophoresis and did not act as a general stress factor. While, increases in the level of extra-cellular synthesis were observed using different magnetic field exposed samples when compared with the control.展开更多
We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which pr...We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which prepared locally with incubation for different period times （24, 48 and 72 hrs） at 37℃. The effects were evaluated by optical density （OD） at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard （0.5）, in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.展开更多
A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to ...A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to obtain specific antibodies in their serum. Serial dilutions of the prepared antibodies were tested directly against samples of bacteria and compared with control samples, which include bacteria that produce Gramicidin (S) (as a positive control) and the bacteria that does not produce Gramicidin (S) (as a negative control) depending slide method after the incubation of bacteria in a suitable media for 3 - 4 hours at 37°C. The prepared kites were evaluated in reference Laboratories and they proved to be highly sensitive (100%), specific (100%), more economic and reliable procedure, in addition to its stability for more than one year. It was the first time to prepare a kit for detection of bacteria which produce Gramicidin (S) directly within few minutes.展开更多
In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nu...In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.展开更多
This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves （Ficus elastica, Philodendrom bipinnatifidum and Aglonema） against some pathogenic bacteria （ Escheri...This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves （Ficus elastica, Philodendrom bipinnatifidum and Aglonema） against some pathogenic bacteria （ Escherichia coli, P. aeruginosa and Staph. Aureus）, and fungi （Microsporum gypseum and Aspergillus flavus）. It was concluded that all tested washing water of the plants leaves secretes exhibited various inhibitory effects, both Philodendrom more antibacterial activity than Ficus elastica. While both E. coli and P bipinnatifidum and Aglonema commutatum had exhibited aeruginosa were being more sensitive than Staph. aureus. The same inhibitory effects were observed when the plants leaves inoculated in their surface with pathogenic bacteria. In contrast to bacterial inhibitory effects, the washing water of natural surface secretes of tested ornamental plants leaves induced mycelium growth of both tested fungi. Microsporum gypseum mycelium growth induced more than Aspergillusflavus especially in case of the washing water of Ficus elastic that have highest effects at 7.5/500 mL of medium. This study concluded the uses of ornamental plants for the indoors and outdoors to control the growth of pathogenic microbes and problems associated with hospital.展开更多
文摘Escherichia coli type 1 was used as a model system to determine whether static magnetic fields are a general stress factor. The bacterial broth culture were exposed to different magnetic force （400, 800, 1200 and 1600 Gauss） with incubation at 37 ℃ for different times （24, 48 and 72 hrs） under aerobic conditions. The response of the cells to the magnetic fields was estimated from the change in total protein synthesis by using spectrophotometer at 550 nm and by using of SDS-PAGE （Sodium dodecyl sulfate- polyacrylamide gel electrophoresis）. Results concluded that was approximately no reproducible changes qualitatively in extracellular proteins were observed in the SDS-PAGE electrophoresis and did not act as a general stress factor. While, increases in the level of extra-cellular synthesis were observed using different magnetic field exposed samples when compared with the control.
文摘We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which prepared locally with incubation for different period times （24, 48 and 72 hrs） at 37℃. The effects were evaluated by optical density （OD） at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard （0.5）, in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.
文摘A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to obtain specific antibodies in their serum. Serial dilutions of the prepared antibodies were tested directly against samples of bacteria and compared with control samples, which include bacteria that produce Gramicidin (S) (as a positive control) and the bacteria that does not produce Gramicidin (S) (as a negative control) depending slide method after the incubation of bacteria in a suitable media for 3 - 4 hours at 37°C. The prepared kites were evaluated in reference Laboratories and they proved to be highly sensitive (100%), specific (100%), more economic and reliable procedure, in addition to its stability for more than one year. It was the first time to prepare a kit for detection of bacteria which produce Gramicidin (S) directly within few minutes.
文摘In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.
文摘This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves （Ficus elastica, Philodendrom bipinnatifidum and Aglonema） against some pathogenic bacteria （ Escherichia coli, P. aeruginosa and Staph. Aureus）, and fungi （Microsporum gypseum and Aspergillus flavus）. It was concluded that all tested washing water of the plants leaves secretes exhibited various inhibitory effects, both Philodendrom more antibacterial activity than Ficus elastica. While both E. coli and P bipinnatifidum and Aglonema commutatum had exhibited aeruginosa were being more sensitive than Staph. aureus. The same inhibitory effects were observed when the plants leaves inoculated in their surface with pathogenic bacteria. In contrast to bacterial inhibitory effects, the washing water of natural surface secretes of tested ornamental plants leaves induced mycelium growth of both tested fungi. Microsporum gypseum mycelium growth induced more than Aspergillusflavus especially in case of the washing water of Ficus elastic that have highest effects at 7.5/500 mL of medium. This study concluded the uses of ornamental plants for the indoors and outdoors to control the growth of pathogenic microbes and problems associated with hospital.
