A new phenolic compound, 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-3-methyl-2H-pyran-2-one(1), along with other six known phenolic derivatives(2-7), were isolated from the mangrove rhizosphere fungus Penicillium jant...A new phenolic compound, 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-3-methyl-2H-pyran-2-one(1), along with other six known phenolic derivatives(2-7), were isolated from the mangrove rhizosphere fungus Penicillium janthinellum HK1-6 cultured in potato dextrose broth medium containing 30 g L^(-1) of natural sea salt. The structure of the new compound(1) was elucidated by comprehensive analysis of spectroscopic data including 1D and 2D NMR spectra. The proposed biosynthetic pathway of compound 1 was also studied in this research. Interestingly, a brominated phenolic derivative, aryl bromide(compound 8), was obtained from this fungal strain cultured in medium containing 30 g L^-1 of NaBr instead of natural sea salt. Compound 8 is proposed as a new natural product and formed through bromination of compound 7 when the fungus was cultured with NaBr. The neuroprotective effect of compound 1 on oxygen-glucose deprivation(OGD)-induced injury was investigated in rat spinal cord astrocytes. MTT assay demonstrated that compound 1 can attenuate OGD-induced cell viability loss in rat spinal cord astrocytes.展开更多
Pavlova sp.is not only one of the most popular microalgae in aquaculture,but also a source of high-quality biodiesel feedstock.With white light as a control(W),Pavlova sp.was cultured in this study under varying light...Pavlova sp.is not only one of the most popular microalgae in aquaculture,but also a source of high-quality biodiesel feedstock.With white light as a control(W),Pavlova sp.was cultured in this study under varying light quality,including monochromatic red light(R),blue light(B),and combinations thereof with different proportions(illuminators of m Rn B,comprised of m red light units and n blue ones,m+n=7),to examine the effects of illuminating light quality on biocomponent production and biodiesel quality.The results show that combined monochromatic light,especially 2R5B,3R4B,4R3B,and 5R2B,could improve the growth of Pavlova sp.The dry weight of harvested algae powder in the 5R2B group reached 418.03 mg/L,and was 22.65%higher than that in the control group(W).Lipid production under combined monochromatic light of 4R3B reached 107.86 mg/L,and was 25.61%higher than in the control(W).In addition,illumination using 4R3B increased the proportion of C16∶0(palmitic acid)and C16:1(palmitoleic acid)fatty acids in Pavlova sp.by 15.55%and 21.94%,respectively,which translates into improved biodiesel quality.All cetane numbers(CN)for 4R3B–6R1B were over 51,while iodine values(IV)and degrees of unsaturation(DU)were reduced,leading to more stable biodiesel suitable for longterm storage.In addition,protein production under 6R1B was as high as 31.56 mg/L,1.8 times greater than under W.Light quality is proposed as an effective tool to regulate biocomponent production by microalgae.展开更多
Fungi isolated from mangrove rhizosphere soil of the South China Sea were investigated for the production of potential antifungal metabolites. With 28 fungal isolates, the strains Penicillium javanicum HK1-23 and P. j...Fungi isolated from mangrove rhizosphere soil of the South China Sea were investigated for the production of potential antifungal metabolites. With 28 fungal isolates, the strains Penicillium javanicum HK1-23 and P. janthinellum HK1-6 showed significant antifungal activities. A bioassay-guided investigation of the two fungal strains led to the isolation of two secondary metabolites, brefeldin A and penicillic acid, with high yields of 143 and 423 mg L^-1, respectively. Penicillic acid showed potent antifungal activities toward Rhizoctonia solani and R. cerealis, with 67.5% and 76% growth inhibition, respectively, at 50 μg mL^-1. Brefeldin A showed strong activity toward R. cerealis, with 56.4% growth inhibition at 50 μg mL^-1. The research highlights the importance of exploring microbes from mangrove rhizosphere soil for the identification of bioactive metabolites for future fungicide development.展开更多
基金supported by the National Natural Science Foundation of China (Nos.81703411, 41830535, U1606403)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (No.2018SD KJ0406-5)+3 种基金the National Science and Technology Major Project for Significant New Drugs Development (No.2018 ZX09735-004)the Program of Open Studio for Druggability Research of Marine Natural Productthe Pilot National Laboratory for Marine Science and Technology (Qingdao, China)Taishan Scholars Program, China。
文摘A new phenolic compound, 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-3-methyl-2H-pyran-2-one(1), along with other six known phenolic derivatives(2-7), were isolated from the mangrove rhizosphere fungus Penicillium janthinellum HK1-6 cultured in potato dextrose broth medium containing 30 g L^(-1) of natural sea salt. The structure of the new compound(1) was elucidated by comprehensive analysis of spectroscopic data including 1D and 2D NMR spectra. The proposed biosynthetic pathway of compound 1 was also studied in this research. Interestingly, a brominated phenolic derivative, aryl bromide(compound 8), was obtained from this fungal strain cultured in medium containing 30 g L^-1 of NaBr instead of natural sea salt. Compound 8 is proposed as a new natural product and formed through bromination of compound 7 when the fungus was cultured with NaBr. The neuroprotective effect of compound 1 on oxygen-glucose deprivation(OGD)-induced injury was investigated in rat spinal cord astrocytes. MTT assay demonstrated that compound 1 can attenuate OGD-induced cell viability loss in rat spinal cord astrocytes.
基金Supported by the National Natural Science Foundation of China (Nos.42177459,41776156,41271521)。
文摘Pavlova sp.is not only one of the most popular microalgae in aquaculture,but also a source of high-quality biodiesel feedstock.With white light as a control(W),Pavlova sp.was cultured in this study under varying light quality,including monochromatic red light(R),blue light(B),and combinations thereof with different proportions(illuminators of m Rn B,comprised of m red light units and n blue ones,m+n=7),to examine the effects of illuminating light quality on biocomponent production and biodiesel quality.The results show that combined monochromatic light,especially 2R5B,3R4B,4R3B,and 5R2B,could improve the growth of Pavlova sp.The dry weight of harvested algae powder in the 5R2B group reached 418.03 mg/L,and was 22.65%higher than that in the control group(W).Lipid production under combined monochromatic light of 4R3B reached 107.86 mg/L,and was 25.61%higher than in the control(W).In addition,illumination using 4R3B increased the proportion of C16∶0(palmitic acid)and C16:1(palmitoleic acid)fatty acids in Pavlova sp.by 15.55%and 21.94%,respectively,which translates into improved biodiesel quality.All cetane numbers(CN)for 4R3B–6R1B were over 51,while iodine values(IV)and degrees of unsaturation(DU)were reduced,leading to more stable biodiesel suitable for longterm storage.In addition,protein production under 6R1B was as high as 31.56 mg/L,1.8 times greater than under W.Light quality is proposed as an effective tool to regulate biocomponent production by microalgae.
基金supported by the National Natural Science Foundation of China (No.81703411)the Natural Science Foundation of Jiangsu Province (Nos.BK20180940, BK20180937)+1 种基金the Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences (No.KLMEES201802)the Taishan Scholars Program, China。
文摘Fungi isolated from mangrove rhizosphere soil of the South China Sea were investigated for the production of potential antifungal metabolites. With 28 fungal isolates, the strains Penicillium javanicum HK1-23 and P. janthinellum HK1-6 showed significant antifungal activities. A bioassay-guided investigation of the two fungal strains led to the isolation of two secondary metabolites, brefeldin A and penicillic acid, with high yields of 143 and 423 mg L^-1, respectively. Penicillic acid showed potent antifungal activities toward Rhizoctonia solani and R. cerealis, with 67.5% and 76% growth inhibition, respectively, at 50 μg mL^-1. Brefeldin A showed strong activity toward R. cerealis, with 56.4% growth inhibition at 50 μg mL^-1. The research highlights the importance of exploring microbes from mangrove rhizosphere soil for the identification of bioactive metabolites for future fungicide development.
