Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzhei...Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzheimer’s disease,in particular the association between changes in peripheral organ metabolism,changes in gut microbial composition,and Alzheimer’s disease development.To do this,we analyzed peripheral organ metabolism and the gut microbiota in amyloid precursor protein-presenilin 1(APP/PS1)transgenic and control mice at 3,6,9,and 12 months of age.Twelve-month-old APP/PS1 mice exhibited cognitive impairment,Alzheimer’s disease-related brain changes,distinctive metabolic disturbances in peripheral organs and fecal samples(as detected by untargeted metabolomics sequencing),and substantial changes in gut microbial composition compared with younger APP/PS1 mice.Notably,a strong correlation emerged between the gut microbiota and kidney metabolism in APP/PS1 mice.These findings suggest that alterations in peripheral organ metabolism and the gut microbiota are closely related to Alzheimer’s disease development,indicating potential new directions for therapeutic strategies.展开更多
Alzheimer's disease,the primary cause of dementia,is characterized by neuropathologies,such as amyloid plaques,synaptic and neuronal degeneration,and neurofibrillary tangles.Although amyloid plaques are the primar...Alzheimer's disease,the primary cause of dementia,is characterized by neuropathologies,such as amyloid plaques,synaptic and neuronal degeneration,and neurofibrillary tangles.Although amyloid plaques are the primary characteristic of Alzheimer's disease in the central nervous system and peripheral organs,targeting amyloid-beta clearance in the central nervous system has shown limited clinical efficacy in Alzheimer's disease treatment.Metabolic abnormalities are commonly observed in patients with Alzheimer's disease.The liver is the primary peripheral organ involved in amyloid-beta metabolism,playing a crucial role in the pathophysiology of Alzheimer's disease.Notably,impaired cholesterol metabolism in the liver may exacerbate the development of Alzheimer's disease.In this review,we explore the underlying causes of Alzheimer's disease and elucidate the role of the liver in amyloid-beta clearance and cholesterol metabolism.Furthermore,we propose that restoring normal cholesterol metabolism in the liver could represent a promising therapeutic strategy for addressing Alzheimer's disease.展开更多
Walnut oil(WO),known for abundant polyunsaturated fatty acids and an array of bioactive substances such as tocopherols,phytosterols,squalene,melatonin,and polyphenols,which is endowed with numerous health advantages.T...Walnut oil(WO),known for abundant polyunsaturated fatty acids and an array of bioactive substances such as tocopherols,phytosterols,squalene,melatonin,and polyphenols,which is endowed with numerous health advantages.The primary objective of this research was to ascertain the impact of WO on cognitive deficits in learning and memory impairment mice caused by scopolamine(SCOP).The Morris water maze and the step-down avoidance test were utilized to assess the memory and learning capabilities.WO notably counteracted the detrimental effects of SCOP on learning and memory in the Morris water maze,as indicated by a reduction in escape latency and swimming distance.Likewise,WO administration led to a notably reduced number of errors in training trial and an increased latency in testing trial when compared to the SCOP group in the step-down avoidance test.Moreover,WO activated the cholinergic system of the brain by upregulating choline acetyltransferase activity and reducing acetylcholinesterase activity.These results suggest that WO has the potential to protect against memory decline in mice,offering a promising strategy for the prevention of memory-related disorders.展开更多
Objective To explore the therapeutic effect and mechanism of Bushen Tiansui Decoction(补肾填髓方,BSTSD)and its active component icariin on Alzheimer’s disease(AD).Methods(i)Animal experiments.This study conducted exp...Objective To explore the therapeutic effect and mechanism of Bushen Tiansui Decoction(补肾填髓方,BSTSD)and its active component icariin on Alzheimer’s disease(AD).Methods(i)Animal experiments.This study conducted experiments using specific pathogen-free(SPF)grade male C57BL/6J wild-type(WT)mice and APP/PS1 double transgenic mice.The animals were divided into three groups:WT group(WT mice,n=5,receiving distilled wa-ter daily),APP/PS1 group(APP/PS1 double transgenic mice,n=5,receiving distilled water daily),and BSTSD group[APP/PS1 double transgenic mice,n=5,treated with BSTSD suspen-sion at a dosage of 27 g/(kg·d)for 90 d].Cognitive function was assessed using the Morris wa-ter maze(MWM).Post-experiment,hippocampal tissues were collected for analysis of pyra-midal cell and synaptic morphology through hematoxylin-eosin(HE)staining and transmis-sion electron microscopy(TEM).(ii)Cell experiments.The HT-22 cells were divided into con-trol group(untreated),Aβ_(25-35) group(treated with 20μmol/L Aβ_(25-35) for 24 h),icariin group(pre-treated with 20μmol/L icariin for 60 min,followed by 20μmol/L Aβ_(25-35) for an additional 24 h),and icariin+LY294002 group[treated with 20μmol/L icariin and 20μmol/L LY294002(an inhibitor of the phosphoinostitide 3-kinases(PI3K)signaling pathway)for 60 min,then exposed to 20μmol/L Aβ_(25-35) for 24 h],and cell viability was measured.Western blot was used to detect the expression levels of synapse-associated proteins[synaptophysin(SYP)and post-synaptic density-95(PSD-95)]and PI3K signaling pathway associated proteins[phosphorylat-ed(p)-PI3K/PI3K,p-protein kinase B(Akt)/Akt,and p-mechanistic target of rapamycin(mTOR)/mTOR].Results(i)Animal experiments.Compared with APP/PS1 group,BSTSD group showed that escape latency was significantly shortened(P<0.01)and the frequency of crossing the origi-nal platform was significantly increased(P<0.01).Morphological observation showed that pyramidal cells in the hippocampal CA1 region were arranged more regularly,nuclear stain-ing was uniform,and vacuole-like changes were reduced after BSTSD treatment.TEM showed that the length of synaptic active zone in BSTSD treatment group was increased com-pared with APP/PS1 group(P<0.01),and the width of synaptic gap was decreased(P<0.01).(ii)Cell experiments.Icariin had no obvious toxicity to HT-22 cells when the concentration was not more than 20μmol/L(P>0.05),and alleviated the cell viability decline induced by Aβ_(25-35)(P<0.01).Western blot results showed that compared with Aβ_(25-35) group,the ratios of p-PI3K/PI3K,p-Akt/Akt and p-mTOR/mTOR in icariin group were significantly increased(P<0.01),while the protein expression levels of SYP and PSD-95 were increased(P<0.01).These effects were blocked by LY294002(P<0.01).Conclusion BSTSD and icariin enhance cognitive function and synaptic integrity in AD mod-els and provide potential therapeutic strategies through activation of the PI3K/Akt/mTOR pathway.展开更多
Acupuncture at Baihui （GV20） and Dazhui （GV14） reduces neuronal loss and attenuates ultra- structural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction thro...Acupuncture at Baihui （GV20） and Dazhui （GV14） reduces neuronal loss and attenuates ultra- structural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 ex- pression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats.展开更多
Acupuncture can reduce cognitive deficits in Alzheimer’s disease.However,whether electroacupuncture can prevent or alleviate the cognitive deficits in animal models of aging remains poorly understood.Studies have sho...Acupuncture can reduce cognitive deficits in Alzheimer’s disease.However,whether electroacupuncture can prevent or alleviate the cognitive deficits in animal models of aging remains poorly understood.Studies have shown that disordered epigenetic modifications play a critical role in age-related cognitive decline.Therefore,we hypothesized that preventive electroacupuncture might improve cognitive functions during aging by regulating epigenetic modifications.A rat model of aging was produced by intraperitoneal injection of 120 mg/kg D-galactose for 8 weeks.Baihui and Shenshu acupoints were stimulated by electroacupuncture for 8 weeks from the first day of D-galactose administration.Preventive electroacupuncture alleviated memory impairment,decreased tau hyperphosphorylation,and reduced glycogen synthase kinase-3βprotein and mRNA expression levels in the brainstem dorsal raphe nucleus,where intracellular neurofibrillary tangle lesions first occur.In addition,the DNA methylation level in the promoter region of the glycogen synthase kinase-3βgene was increased.The effects of preventive electroacupuncture were stronger than those of preventive acupuncture.Intraperitoneal injection of 0.4 mg/kg 5-aza-2ʹ-deoxycytidine,an inhibitor of DNA methyltransferase that blocks epigenetic modifications,antagonized the effects of preventive electroacupuncture.Our results suggest that preventive electroacupuncture treatment alleviates cognitive impairment in aging rats probably by affecting the epigenetic modification of the glycogen synthase kinase-3βgene in the dorsal raphe nucleus.This study was approved by the Animal Ethics Committee of Hubei University of Chinese Medicine,China(approval No.HUCMS201712001)on November 28,2017.展开更多
The clinical efficacy was compared between 3D navigation-assisted percutaneous iliosacral screw(3DPS)and minimally invasive reconstruction plate(MIRP)in treating sacroiliac complex injury and the surgical procedures o...The clinical efficacy was compared between 3D navigation-assisted percutaneous iliosacral screw(3DPS)and minimally invasive reconstruction plate(MIRP)in treating sacroiliac complex injury and the surgical procedures of 3DPS were introduced.A retrospective analysis was performed on 49 patients with sacroiliac complex injury from March 2013 to May 2017.Twenty-one cases were treated by 3DPS,and 28 cases by MIRP.Intraoperative indexes as operative time,blood loss,incision length,length of hospital stay and postoperative complications were respectively documented.Quality of reduction was postoperatively evaluated by Matta radiological criteria,and clinical effect was assessed by Majeed scoring criteria at the last followup.Operative time and hospital stay were significantly shortened,and blood loss,and incision length were significantly reduced in 3DPS group as compared with those in MIRP group(P<0.05).No statistically significant difference was found between 3DPS group and MIRP group in the assessment of reduction and function(P>0.05).It was concluded that both 3DPS and MIRP can effectively treat the sacroiliac complex injury,and 3DPS can provide an accurate,safe and minimally invasive fixation with shorter operative time and hospital stay.展开更多
Summary:Nurses'work-related fatigue has been recognized as a threat to nurse health and patient safety.The aim of this study was to assess the prevalence of fatigue among first-line nurses combating with COVID-19 ...Summary:Nurses'work-related fatigue has been recognized as a threat to nurse health and patient safety.The aim of this study was to assess the prevalence of fatigue among first-line nurses combating with COVID-19 in Wuhan,China,and to analyze its influencing factors on fatigue.A multi-center,descriptive,cross-sectional design with a convenience sample was used.The statistical population consisted of the first-line nurses in 7 tertiary general hospitals from March 3,2020 to March 10,2020 in Wuhan of China.A total of 2667 samples from 2768 contacted participants completed the investgation,with a response rate of 96.35%.Social-demographic questionnaire,work-related questionnaire,Fatigue Scale-14,Generalized Anxiety Disorder-7,Patient Health Questionnaire-9,and Chinese Perceived Stress Scale were used to conduct online survey.The descriptive statistic of nurses'social-demographic characteristics was conducted,and the related variables of work,anxiety,depression,perceived stress and fatigue were analyzed by Z-tests,nonparametric test and Pearson's correlation analysis.The significant factors which resulted in nurses'fatigue were further analyzed by multiple linear regression analysis.The median score for the first-line nurses'fatigue in Wuhan was 4(2,8).The median score of physical and mental fatigue of them was 3(1,6)and 1(0,3)respectively.According to the scoring criteria,35.06%nurses(w=935)of all participants were in the fatigue status,their median score of fatigue was 10(8,11),and the median score of physical and mental fatigue of them was 7(5,8)and 3(2,4)respectively.Multiple linear regression analysis revealed the participants in the risk groups of anxiety,depression and perceived stress had higher scores on physical and mental fatigue and the statistically significant positive correlation was observed between the variables and nurses'fatigue,the frequency of exercise and nurses'fatigue had a statistically significant negative correlation,and average daily working hours had a significantly positive correlation with nurses'fatigue,and the frequency of weekly night shift had a low positive correlation with nurses'fatigue(P<0.01).There was a moderate level of fatigue among the first-line nurses fighting against COVID-19 pandemic in Wuhan,China.Government and health authorities need to formulate and take effective intervention strategies according to the relevant risk factors,and undertake preventive measures aimed at reducing health hazards due to increased work-related fatigue among first-line nurses,and to enhance their health status and provide a safe occupational environment worldwide.Promoting both medical and nursing safety while combating with the pandemic currently is warranted.展开更多
Objective:To study the relationship between malignant biological molecule expression during the pathological process of gastric cancer and helicobacter pylori (HP) infection.Methods:A total of 78 cases of gastric canc...Objective:To study the relationship between malignant biological molecule expression during the pathological process of gastric cancer and helicobacter pylori (HP) infection.Methods:A total of 78 cases of gastric cancer specimens that were surgically removed in our hospital between May 2013 and June 2015 were selected as the research material, the gastric cancer tissue and the tissue adjacent to carcinoma were collected, rapid urease test kits were used to test HP, and fluorescent quantitative PCR kits were used to determine the mRNA expression of proliferation- and invasion-related genes.Results:EZH2, c-myc, PIK3CD, Vav3 and UHRF1 mRNA expression in gastric cancer tissue were significantly higher than those in the tissue adjacent to carcinoma while Mst1, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in the tissue adjacent to carcinoma;EZH2, c-myc, PIK3CD, Vav3 and UHRF1 mRNA expression in HP-positive gastric cancer tissue were significantly higher than those in HP-negative gastric cancer tissue while Mst1, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in HP-negative gastric cancer tissue.Conclusion: Helicobacter pylori have regulating effect on the expression of proliferation- and invasion-related malignant biological molecules during the pathological process of gastric cancer.展开更多
Objective:Patients undergoing hematopoietic stem cell transplant(HSCT)need frequent transfusions,until their red blood cells(RBCs)and platelets start to recover.The safe transfusion for patients who receive ABO-incomp...Objective:Patients undergoing hematopoietic stem cell transplant(HSCT)need frequent transfusions,until their red blood cells(RBCs)and platelets start to recover.The safe transfusion for patients who receive ABO-incompatible HSCT is essential to the transplant process.To date,there is no user-friendly tool to choose the right blood product for transfusion treatment,despite the number of guidelines and expert advice on the subject.Methods:R/shiny is a powerful programming language for clinical data analysis and visualization.It can create interactive web applications that work in real-time.The web application named TSR was built using R programming,simplifying blood transfusion practice for ABO-incompatible HSCT witha one-click solution.Results:The TSR is divided into four main tabs.The home tab provides an overview of the application,while RBC,plasma and platelet transfusion tabs offer tailored suggestions for blood product selection in each category.Unlike traditional methods that rely on treatment guidelines and specialist consensus,TSR leverages the power of the R/Shiny interface to extract critical content based on user-specified parameters,providing an innovative approach to improve transfusion support.Conclusion:The present study highlights that the TSR enables real-time analysis,and promotes transfusion practice byoffering a unique and efficient one-key output for blood product selection to ABO-incompatible HSCT.TSR has the potential to become a widely-utilized tool for transfusion services,providing a reliable and user-friendly solution that enhances transfusion safety in clinical practice.展开更多
The aim of this study was to estimate the seroprevalence of immunoglobulin M(IgM)and G(IgG)antibodies against SARS-CoV-2 in asymptomatic people in Wuhan.This was a cross-sectional study,which enrolled 18,712 asymptoma...The aim of this study was to estimate the seroprevalence of immunoglobulin M(IgM)and G(IgG)antibodies against SARS-CoV-2 in asymptomatic people in Wuhan.This was a cross-sectional study,which enrolled 18,712 asymptomatic participants from 154 work units in Wuhan.Pearson Chi-square test,t-test,and Mann-Whitney test were used to compare the standardized seroprevalence of IgG and IgM for age and gender between different groups.展开更多
Objective:To demonstrate the advantages of scraping in helping COVID-19 patients in convalescence,popularize scraping therapy among them and provide basis for the establishment of operation specification.Methods:Discu...Objective:To demonstrate the advantages of scraping in helping COVID-19 patients in convalescence,popularize scraping therapy among them and provide basis for the establishment of operation specification.Methods:Discuss the relationship between scraping and epidemic disease by reviewing the relevant scraping theory analysis,summarize the existing clinical studies and propose reference scheme for the treatment of COVID-19 patients in convalescence with respect to the related symptoms proving effective treatment of scraping on patients in convalescence.Results:Ancient healers used scraping to treat pestilence,and there have been many clinical trials and experiments for the treatment of convalescent symptoms related to scraping.Scraping also has the effect of improving immune function and antioxidation,which is feasible for the treatment of convalescent symptoms related to scraping.Conclusion:Scraping can effectively improve the clinical symptoms of COVID-19 convalescent patients,and it can be used as one of the external treatments for convalescent patients.It is recommended to promote scraping among the patients so that they can have self-intervention treatment.展开更多
Objective:To study the correlation between magnetic resonance diffusion-weighted imaging ADC value of endometrial stromal sarcoma and the malignant biological features.Methods:A total of 34 patients with endometrial s...Objective:To study the correlation between magnetic resonance diffusion-weighted imaging ADC value of endometrial stromal sarcoma and the malignant biological features.Methods:A total of 34 patients with endometrial stromal sarcoma who received surgical resection in Hubei Provincial Hospital of Integrated Chinese & Western Medicine between May 2014 and August 2016 were selected as the malignant group of the research, and 58 patients with uterine fibroids who received surgical resection between August 2015 and October 2016 were selected as the control group of the research. Magnetic resonance diffusion-weighted imaging was done before operation to measure apparent diffusion coefficient (ADC value). The lesions were collected after operation to determine the expression of proliferation genes as well as estrogen and progestogen receptors.Results:Endometrial stromal sarcoma ADC value of malignant group was significantly lower than uterine fibroid ADC value of control group;CyclinD1, Rb, Sp1, Survivin, ER , ERβ, PRA and PRB protein expression in endometrial stromal sarcoma lesions of malignant group were significantly higher than those of control group while SULT1E1 protein expression was significantly lower than that of control group;CyclinD1, Rb, Sp1, Survivin, ER , ERβ, PRA and PRB protein expression in endometrial stromal sarcoma lesions of subgroup with low ADC value were significantly higher than those of subgroup with high ADC value while SULT1E1 protein expression was significantly lower than that of subgroup with high ADC value.Conclusion: Magnetic resonance diffusion weighted imaging ADC values can be used to evaluate the malignant biological behavior of endometrial stromal sarcoma.展开更多
What is already known about this topic?Lower extremity musculoskeletal diseases(LE-MSDs)have emerged as a significant contributor to the global disease economic burden and worker absenteeism,becoming a global public h...What is already known about this topic?Lower extremity musculoskeletal diseases(LE-MSDs)have emerged as a significant contributor to the global disease economic burden and worker absenteeism,becoming a global public health concern.However,the epidemic characteristics of LE-MSDs among occupational populations in China are unknown.What is added by this report?This report finds that the LE-MSDs prevalence rate among key occupational groups in China is 17.7%,with the top 5 being toy manufacturing,medical personnel,automobile manufacturing,nonferrous metal smelting and rolling processing,and coal mining and washing.What are the implications for public health practice?This study investigated the occurrence of LE-MSDs in key industries in China and its possible risk factors to provide big data support for preventing and controlling such diseases in these industries.展开更多
To the Editor:Lumbar spinal canal stenosis(LSCS)is a common disease among older individuals.Hypertrophic lumbar ligamentum flavum(LLF)plays a vital role in the pathogenesis of LSCS.The physiological LLF is a kind of d...To the Editor:Lumbar spinal canal stenosis(LSCS)is a common disease among older individuals.Hypertrophic lumbar ligamentum flavum(LLF)plays a vital role in the pathogenesis of LSCS.The physiological LLF is a kind of dense connective tissue with thickness of 2 to 4mm,which is mainly composed of fibroblasts and extracellular matrix(ECM).ECM contains elastic,collagen fibers and ground substances.The distribution characteristics of fibers show that a large number of elastic fibers are mixed with a small amount of the collagen fibers;mainly type I and type III collagen fibers.These fibers are arranged tightly and regularly along the direction of column’s axis.The LLF is located in the dorsal part of the dura mater of the spinal canal,and it attaches to the upper border and lower parts of anterior surfaces of the laminas.The hypotrophy of LLF is the consequence of several factors such as aging,inflammatory process,stress loading and genetic predisposition.展开更多
OBJECTIVE: To observe the effect of Qibai Pingfei capsule(QBPF) medicated serum on the proliferation of rat pulmonary arterial smooth muscle cells(PASMCs) under hypoxia conditions and to investigate its key molecular ...OBJECTIVE: To observe the effect of Qibai Pingfei capsule(QBPF) medicated serum on the proliferation of rat pulmonary arterial smooth muscle cells(PASMCs) under hypoxia conditions and to investigate its key molecular effects on the Ca^(2+)/calcineurin/nuclear factor of activated T-cells 3(NFATc3) signaling pathway.METHODS: QBPF was provided to rats via continuous gavage for 10 days. Primary rat PASMCs were cultured using the direct adherent culture method.Methyl thiazolyl tetrazolium assay was used to eval-uate the effect of QBPF on PASMCs proliferation under hypoxia conditions. Laser scanning confocal microscopy was used to detect changes in intracellular free calcium([Ca^(2+)]_i) in PASMC-loaded Fluo-3-AM.Real-time quantitative polymerase chain reaction and western blot were used to detect the transcription and protein expression levels of calcineurin and NFATc3 genes in PASMCs.RESULTS: Compared with normoxia conditions,PASMCs proliferated at 12, 24, 48, and 72 h under hypoxia conditions. QBPF at concentrations of 5%,10%, and 20% could inhibit hypoxia-induced PASMC proliferation to different degrees. The inhibitory effect was most significant in the 20% QBPF group under 24 h hypoxia conditions. The concentration of [Ca^(2+)]_iin PASMCs under hypoxia was increased and [Ca^(2+)]_iwas significantly decreased when co-incubated with QBPF at 5%, 10%, and 20%. Compared with normoxia conditions, the m RNA and protein expression levels of calcineurin and NFATc3 in PASMCs induced by hypoxia were up-regulated.QBPF application significantly down-regulated m RNA and protein expression levels of calcineurin and NFATc3 in PASMCs.CONCLUSION: QBPF can effectively inhibit hypoxia-induced proliferation of PASMCs through down-regulation of key molecular expression via the Ca^(2+)/calcineurin/NFATc3 pathway.展开更多
Objective:Bushen Tiansui formula(BSTSF),a traditional Chinese medicine prescription,has been widely used to treat Alzheimer’s disease(AD).However,the mechanisms underlying its effects remain largely unknown.In this s...Objective:Bushen Tiansui formula(BSTSF),a traditional Chinese medicine prescription,has been widely used to treat Alzheimer’s disease(AD).However,the mechanisms underlying its effects remain largely unknown.In this study,a rat AD model was used to study the effects of BSTSF on cognitive performance and expression of transfer RNA-derived small RNAs(tsRNAs)in the hippocampus,to determine whether treatment of AD with BSTSF could regulate the expression of tsRNAs,a novel small non-coding RNA.Methods:To generate a validated AD model,oligomeric amyloid-β_(1-42)(Aβ_(1-42))was injected intracerebroventricularly into rats.The Morris water maze(MWM)test was used to evaluate rat cognitive performance,and tsRNA-sequencing was conducted to examine tsRNA expression in the rat hippocampus.Potential targets were validated by quantitative real-time polymerase chain reaction(qRT-PCR).Bioinformatic analyses were conducted to investigate the biological function of candidate tsRNAs.Results:The learning and memory deficits of Aβ_(1-42)-induced AD rats,assessed by MWM tests,were clearly ameliorated by BSTSF treatment.A total of 387 tsRNAs were detected in the rat hippocampus.Among them,13 were significantly dysregulated in AD rats compared with sham control rats,while 57 were markedly altered by BSTSF treatment,relative to untreated AD rats(fold change>2 and P<0.05).Moreover,six BSTSF treatment-related tsRNAs were identified and validated by qRT-PCR.Bioinformatic analyses indicated that the six treatment-related tsRNAs had potential therapeutic roles,via multiple signaling pathways and Gene Ontology biological functions,including cyclic adenosine monophosphate and retrograde endocannabinoid signaling.Conclusion:This study identified a previously uncharacterized mechanism underlying the effects of BSTSF in alleviating the learning and memory deficits in Aβ_(1-42)-induced AD rats,demonstrating that tsRNAs are potential therapeutic targets of BSTSF in the treatment of AD.展开更多
Alzheimer’s disease(AD)is a progressive and fatal neurodegenerative condition and the most prevalent cause of dementia.This disease is characterized by progressive cognitive impairment.The prevalence of AD is current...Alzheimer’s disease(AD)is a progressive and fatal neurodegenerative condition and the most prevalent cause of dementia.This disease is characterized by progressive cognitive impairment.The prevalence of AD is currently affecting more than 35 million people and is rising worldwide.No efficient therapy is currently available due to low drug potency and a number of various obstacles to delivery.Recent nanotechnological advancements have the potential to offer promising therapeutic options.Progress on nanomaterials as well as their applications in biomedicine is receiving increasing attention,especially the advantages of nanomaterial-based drug delivery systems.The aim of this review is to comprehensively summarize the latest developments in nanomaterial-based strategies for AD treatment,including nanoparticles,liposomes and other options for the delivery of therapeutic compounds and scaffolds for cell delivery strategies.Future research directions are also proposed.We hope this review can provide important information to guide the future development of nanomaterials in AD treatment.展开更多
Summary What is already known about this topic?In recent decades,work-related musculoskeletal disorders(WMSDs)have become increasingly prominent and have become an important issue that is of universal concern and an u...Summary What is already known about this topic?In recent decades,work-related musculoskeletal disorders(WMSDs)have become increasingly prominent and have become an important issue that is of universal concern and an urgent need to be solved in all countries of the world.What is added by this report?The top three industries or occupational groups with the highest standardized prevalence rate of WMSDs were flight attendants,medical staff,and vegetable greenhouses in that order.Women workers were 1.5 times more likely to suffer from WMSDs than men workers.展开更多
What is already known about this topic?Work-related musculoskeletal disorders(WMSDs)have a high prevalence and seriously harmful,which has attracted extensive attention in various countries in the world.Currently,the ...What is already known about this topic?Work-related musculoskeletal disorders(WMSDs)have a high prevalence and seriously harmful,which has attracted extensive attention in various countries in the world.Currently,the occurrence and rules of WMSDs in key industries are not known in China.What is added by this report?The prevalence of WMSDs is relatively high among professional populations in key industries in China,with the most commonly affected body parts concentrated in neck,shoulders,and low back and increasing with age and working years.What are the implications for public health practice?This study determined the prevalence and distribution characteristics of WMSDs in key industries in China and provided scientific evidence to recommend for inclusion of WMSDs in the new revision of the list of occupational diseases in China.展开更多
基金financially supported by the National Natural Science Foundation of China,No.823 74552 (to WP)the Science and Technology Innovation Program of Hunan Province,No.2022RC1220 (to WP)+1 种基金the Natural Science Foundation of Hunan Province of China,Nos.2020JJ4803 (to WP),2022JJ40723 (to MY)the Scientific Research Launch Project for New Employees of the Second Xiangya Hospital of Central South University (to MY)
文摘Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzheimer’s disease,in particular the association between changes in peripheral organ metabolism,changes in gut microbial composition,and Alzheimer’s disease development.To do this,we analyzed peripheral organ metabolism and the gut microbiota in amyloid precursor protein-presenilin 1(APP/PS1)transgenic and control mice at 3,6,9,and 12 months of age.Twelve-month-old APP/PS1 mice exhibited cognitive impairment,Alzheimer’s disease-related brain changes,distinctive metabolic disturbances in peripheral organs and fecal samples(as detected by untargeted metabolomics sequencing),and substantial changes in gut microbial composition compared with younger APP/PS1 mice.Notably,a strong correlation emerged between the gut microbiota and kidney metabolism in APP/PS1 mice.These findings suggest that alterations in peripheral organ metabolism and the gut microbiota are closely related to Alzheimer’s disease development,indicating potential new directions for therapeutic strategies.
基金financially supported by the Science and Technology Innovation Program of Hunan Province,No.2022RC1220(to WP)China Postdoctoral Science Foundation,No.2022M711733(to ZZ)+2 种基金the National Natural Science Foundation of China,No.82160920(to ZZ)Hebei Postdoctoral Scientific Research Project,No.B2022003040(to ZZ)Hunan Flagship Department of Integrated Traditional Chinese and Western Medicine(to WP)。
文摘Alzheimer's disease,the primary cause of dementia,is characterized by neuropathologies,such as amyloid plaques,synaptic and neuronal degeneration,and neurofibrillary tangles.Although amyloid plaques are the primary characteristic of Alzheimer's disease in the central nervous system and peripheral organs,targeting amyloid-beta clearance in the central nervous system has shown limited clinical efficacy in Alzheimer's disease treatment.Metabolic abnormalities are commonly observed in patients with Alzheimer's disease.The liver is the primary peripheral organ involved in amyloid-beta metabolism,playing a crucial role in the pathophysiology of Alzheimer's disease.Notably,impaired cholesterol metabolism in the liver may exacerbate the development of Alzheimer's disease.In this review,we explore the underlying causes of Alzheimer's disease and elucidate the role of the liver in amyloid-beta clearance and cholesterol metabolism.Furthermore,we propose that restoring normal cholesterol metabolism in the liver could represent a promising therapeutic strategy for addressing Alzheimer's disease.
基金supported by the Key Technology R&D Program of Shandong Province(2023TZXD069)the National Natural Science Foundation of China(NSFC-U21A20274)+2 种基金the innovation group project of Hubei Province(2023AFA042)the Earmarked Fund for China Agriculture Research System(CARS-14)by Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2016-OCRI).
文摘Walnut oil(WO),known for abundant polyunsaturated fatty acids and an array of bioactive substances such as tocopherols,phytosterols,squalene,melatonin,and polyphenols,which is endowed with numerous health advantages.The primary objective of this research was to ascertain the impact of WO on cognitive deficits in learning and memory impairment mice caused by scopolamine(SCOP).The Morris water maze and the step-down avoidance test were utilized to assess the memory and learning capabilities.WO notably counteracted the detrimental effects of SCOP on learning and memory in the Morris water maze,as indicated by a reduction in escape latency and swimming distance.Likewise,WO administration led to a notably reduced number of errors in training trial and an increased latency in testing trial when compared to the SCOP group in the step-down avoidance test.Moreover,WO activated the cholinergic system of the brain by upregulating choline acetyltransferase activity and reducing acetylcholinesterase activity.These results suggest that WO has the potential to protect against memory decline in mice,offering a promising strategy for the prevention of memory-related disorders.
基金Hunan Provincial Natural Science Foundation of China(2022JJ40220)Health Commission of Hunan Province(B202303106781)Hunan Administration of Traditional Chinese Medicine(2021192).
文摘Objective To explore the therapeutic effect and mechanism of Bushen Tiansui Decoction(补肾填髓方,BSTSD)and its active component icariin on Alzheimer’s disease(AD).Methods(i)Animal experiments.This study conducted experiments using specific pathogen-free(SPF)grade male C57BL/6J wild-type(WT)mice and APP/PS1 double transgenic mice.The animals were divided into three groups:WT group(WT mice,n=5,receiving distilled wa-ter daily),APP/PS1 group(APP/PS1 double transgenic mice,n=5,receiving distilled water daily),and BSTSD group[APP/PS1 double transgenic mice,n=5,treated with BSTSD suspen-sion at a dosage of 27 g/(kg·d)for 90 d].Cognitive function was assessed using the Morris wa-ter maze(MWM).Post-experiment,hippocampal tissues were collected for analysis of pyra-midal cell and synaptic morphology through hematoxylin-eosin(HE)staining and transmis-sion electron microscopy(TEM).(ii)Cell experiments.The HT-22 cells were divided into con-trol group(untreated),Aβ_(25-35) group(treated with 20μmol/L Aβ_(25-35) for 24 h),icariin group(pre-treated with 20μmol/L icariin for 60 min,followed by 20μmol/L Aβ_(25-35) for an additional 24 h),and icariin+LY294002 group[treated with 20μmol/L icariin and 20μmol/L LY294002(an inhibitor of the phosphoinostitide 3-kinases(PI3K)signaling pathway)for 60 min,then exposed to 20μmol/L Aβ_(25-35) for 24 h],and cell viability was measured.Western blot was used to detect the expression levels of synapse-associated proteins[synaptophysin(SYP)and post-synaptic density-95(PSD-95)]and PI3K signaling pathway associated proteins[phosphorylat-ed(p)-PI3K/PI3K,p-protein kinase B(Akt)/Akt,and p-mechanistic target of rapamycin(mTOR)/mTOR].Results(i)Animal experiments.Compared with APP/PS1 group,BSTSD group showed that escape latency was significantly shortened(P<0.01)and the frequency of crossing the origi-nal platform was significantly increased(P<0.01).Morphological observation showed that pyramidal cells in the hippocampal CA1 region were arranged more regularly,nuclear stain-ing was uniform,and vacuole-like changes were reduced after BSTSD treatment.TEM showed that the length of synaptic active zone in BSTSD treatment group was increased com-pared with APP/PS1 group(P<0.01),and the width of synaptic gap was decreased(P<0.01).(ii)Cell experiments.Icariin had no obvious toxicity to HT-22 cells when the concentration was not more than 20μmol/L(P>0.05),and alleviated the cell viability decline induced by Aβ_(25-35)(P<0.01).Western blot results showed that compared with Aβ_(25-35) group,the ratios of p-PI3K/PI3K,p-Akt/Akt and p-mTOR/mTOR in icariin group were significantly increased(P<0.01),while the protein expression levels of SYP and PSD-95 were increased(P<0.01).These effects were blocked by LY294002(P<0.01).Conclusion BSTSD and icariin enhance cognitive function and synaptic integrity in AD mod-els and provide potential therapeutic strategies through activation of the PI3K/Akt/mTOR pathway.
基金financially sponsored by the Foundation for Excellent Young Talents in Universities of Anhui Province in China,No.2010SQRL105the National Natural Science Foundation of China,No.81173325
文摘Acupuncture at Baihui （GV20） and Dazhui （GV14） reduces neuronal loss and attenuates ultra- structural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 ex- pression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats.
基金This work was supported by the National Natural Science Foundation of China,Nos.81373741(to LHK),81473786(to YJD),81873380(to YJD)Sun Guojie Inheritance Base for TCM Acupuncture-Moxibustion of World Federation of Acupuncture-Moxibustion Societies in Wuhan,China(World Federation of Acupuncture-Moxibustion Societies[2019]No.26)(to YJD)and“Wei Yizong Chiropractic Team”Project Founded by Shenzhen of China,No.SZSM201612059(to YFL).
文摘Acupuncture can reduce cognitive deficits in Alzheimer’s disease.However,whether electroacupuncture can prevent or alleviate the cognitive deficits in animal models of aging remains poorly understood.Studies have shown that disordered epigenetic modifications play a critical role in age-related cognitive decline.Therefore,we hypothesized that preventive electroacupuncture might improve cognitive functions during aging by regulating epigenetic modifications.A rat model of aging was produced by intraperitoneal injection of 120 mg/kg D-galactose for 8 weeks.Baihui and Shenshu acupoints were stimulated by electroacupuncture for 8 weeks from the first day of D-galactose administration.Preventive electroacupuncture alleviated memory impairment,decreased tau hyperphosphorylation,and reduced glycogen synthase kinase-3βprotein and mRNA expression levels in the brainstem dorsal raphe nucleus,where intracellular neurofibrillary tangle lesions first occur.In addition,the DNA methylation level in the promoter region of the glycogen synthase kinase-3βgene was increased.The effects of preventive electroacupuncture were stronger than those of preventive acupuncture.Intraperitoneal injection of 0.4 mg/kg 5-aza-2ʹ-deoxycytidine,an inhibitor of DNA methyltransferase that blocks epigenetic modifications,antagonized the effects of preventive electroacupuncture.Our results suggest that preventive electroacupuncture treatment alleviates cognitive impairment in aging rats probably by affecting the epigenetic modification of the glycogen synthase kinase-3βgene in the dorsal raphe nucleus.This study was approved by the Animal Ethics Committee of Hubei University of Chinese Medicine,China(approval No.HUCMS201712001)on November 28,2017.
文摘The clinical efficacy was compared between 3D navigation-assisted percutaneous iliosacral screw(3DPS)and minimally invasive reconstruction plate(MIRP)in treating sacroiliac complex injury and the surgical procedures of 3DPS were introduced.A retrospective analysis was performed on 49 patients with sacroiliac complex injury from March 2013 to May 2017.Twenty-one cases were treated by 3DPS,and 28 cases by MIRP.Intraoperative indexes as operative time,blood loss,incision length,length of hospital stay and postoperative complications were respectively documented.Quality of reduction was postoperatively evaluated by Matta radiological criteria,and clinical effect was assessed by Majeed scoring criteria at the last followup.Operative time and hospital stay were significantly shortened,and blood loss,and incision length were significantly reduced in 3DPS group as compared with those in MIRP group(P<0.05).No statistically significant difference was found between 3DPS group and MIRP group in the assessment of reduction and function(P>0.05).It was concluded that both 3DPS and MIRP can effectively treat the sacroiliac complex injury,and 3DPS can provide an accurate,safe and minimally invasive fixation with shorter operative time and hospital stay.
文摘Summary:Nurses'work-related fatigue has been recognized as a threat to nurse health and patient safety.The aim of this study was to assess the prevalence of fatigue among first-line nurses combating with COVID-19 in Wuhan,China,and to analyze its influencing factors on fatigue.A multi-center,descriptive,cross-sectional design with a convenience sample was used.The statistical population consisted of the first-line nurses in 7 tertiary general hospitals from March 3,2020 to March 10,2020 in Wuhan of China.A total of 2667 samples from 2768 contacted participants completed the investgation,with a response rate of 96.35%.Social-demographic questionnaire,work-related questionnaire,Fatigue Scale-14,Generalized Anxiety Disorder-7,Patient Health Questionnaire-9,and Chinese Perceived Stress Scale were used to conduct online survey.The descriptive statistic of nurses'social-demographic characteristics was conducted,and the related variables of work,anxiety,depression,perceived stress and fatigue were analyzed by Z-tests,nonparametric test and Pearson's correlation analysis.The significant factors which resulted in nurses'fatigue were further analyzed by multiple linear regression analysis.The median score for the first-line nurses'fatigue in Wuhan was 4(2,8).The median score of physical and mental fatigue of them was 3(1,6)and 1(0,3)respectively.According to the scoring criteria,35.06%nurses(w=935)of all participants were in the fatigue status,their median score of fatigue was 10(8,11),and the median score of physical and mental fatigue of them was 7(5,8)and 3(2,4)respectively.Multiple linear regression analysis revealed the participants in the risk groups of anxiety,depression and perceived stress had higher scores on physical and mental fatigue and the statistically significant positive correlation was observed between the variables and nurses'fatigue,the frequency of exercise and nurses'fatigue had a statistically significant negative correlation,and average daily working hours had a significantly positive correlation with nurses'fatigue,and the frequency of weekly night shift had a low positive correlation with nurses'fatigue(P<0.01).There was a moderate level of fatigue among the first-line nurses fighting against COVID-19 pandemic in Wuhan,China.Government and health authorities need to formulate and take effective intervention strategies according to the relevant risk factors,and undertake preventive measures aimed at reducing health hazards due to increased work-related fatigue among first-line nurses,and to enhance their health status and provide a safe occupational environment worldwide.Promoting both medical and nursing safety while combating with the pandemic currently is warranted.
文摘Objective:To study the relationship between malignant biological molecule expression during the pathological process of gastric cancer and helicobacter pylori (HP) infection.Methods:A total of 78 cases of gastric cancer specimens that were surgically removed in our hospital between May 2013 and June 2015 were selected as the research material, the gastric cancer tissue and the tissue adjacent to carcinoma were collected, rapid urease test kits were used to test HP, and fluorescent quantitative PCR kits were used to determine the mRNA expression of proliferation- and invasion-related genes.Results:EZH2, c-myc, PIK3CD, Vav3 and UHRF1 mRNA expression in gastric cancer tissue were significantly higher than those in the tissue adjacent to carcinoma while Mst1, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in the tissue adjacent to carcinoma;EZH2, c-myc, PIK3CD, Vav3 and UHRF1 mRNA expression in HP-positive gastric cancer tissue were significantly higher than those in HP-negative gastric cancer tissue while Mst1, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in HP-negative gastric cancer tissue.Conclusion: Helicobacter pylori have regulating effect on the expression of proliferation- and invasion-related malignant biological molecules during the pathological process of gastric cancer.
文摘Objective:Patients undergoing hematopoietic stem cell transplant(HSCT)need frequent transfusions,until their red blood cells(RBCs)and platelets start to recover.The safe transfusion for patients who receive ABO-incompatible HSCT is essential to the transplant process.To date,there is no user-friendly tool to choose the right blood product for transfusion treatment,despite the number of guidelines and expert advice on the subject.Methods:R/shiny is a powerful programming language for clinical data analysis and visualization.It can create interactive web applications that work in real-time.The web application named TSR was built using R programming,simplifying blood transfusion practice for ABO-incompatible HSCT witha one-click solution.Results:The TSR is divided into four main tabs.The home tab provides an overview of the application,while RBC,plasma and platelet transfusion tabs offer tailored suggestions for blood product selection in each category.Unlike traditional methods that rely on treatment guidelines and specialist consensus,TSR leverages the power of the R/Shiny interface to extract critical content based on user-specified parameters,providing an innovative approach to improve transfusion support.Conclusion:The present study highlights that the TSR enables real-time analysis,and promotes transfusion practice byoffering a unique and efficient one-key output for blood product selection to ABO-incompatible HSCT.TSR has the potential to become a widely-utilized tool for transfusion services,providing a reliable and user-friendly solution that enhances transfusion safety in clinical practice.
基金supported by Advisory Research Project of the Chinese Academy of Engineering in 2019[2019-XZ-70]。
文摘The aim of this study was to estimate the seroprevalence of immunoglobulin M(IgM)and G(IgG)antibodies against SARS-CoV-2 in asymptomatic people in Wuhan.This was a cross-sectional study,which enrolled 18,712 asymptomatic participants from 154 work units in Wuhan.Pearson Chi-square test,t-test,and Mann-Whitney test were used to compare the standardized seroprevalence of IgG and IgM for age and gender between different groups.
基金the National Key Research and Development Plan for TCM modernization research special 4.5.2 project task No.5 subtask(project number2018YFC1707705)Project commissioned by the Department of Policy,Regulation and Supervision,National Administration of Traditional Chinese Medicine(GZY-FJS-2018-023)。
文摘Objective:To demonstrate the advantages of scraping in helping COVID-19 patients in convalescence,popularize scraping therapy among them and provide basis for the establishment of operation specification.Methods:Discuss the relationship between scraping and epidemic disease by reviewing the relevant scraping theory analysis,summarize the existing clinical studies and propose reference scheme for the treatment of COVID-19 patients in convalescence with respect to the related symptoms proving effective treatment of scraping on patients in convalescence.Results:Ancient healers used scraping to treat pestilence,and there have been many clinical trials and experiments for the treatment of convalescent symptoms related to scraping.Scraping also has the effect of improving immune function and antioxidation,which is feasible for the treatment of convalescent symptoms related to scraping.Conclusion:Scraping can effectively improve the clinical symptoms of COVID-19 convalescent patients,and it can be used as one of the external treatments for convalescent patients.It is recommended to promote scraping among the patients so that they can have self-intervention treatment.
文摘Objective:To study the correlation between magnetic resonance diffusion-weighted imaging ADC value of endometrial stromal sarcoma and the malignant biological features.Methods:A total of 34 patients with endometrial stromal sarcoma who received surgical resection in Hubei Provincial Hospital of Integrated Chinese & Western Medicine between May 2014 and August 2016 were selected as the malignant group of the research, and 58 patients with uterine fibroids who received surgical resection between August 2015 and October 2016 were selected as the control group of the research. Magnetic resonance diffusion-weighted imaging was done before operation to measure apparent diffusion coefficient (ADC value). The lesions were collected after operation to determine the expression of proliferation genes as well as estrogen and progestogen receptors.Results:Endometrial stromal sarcoma ADC value of malignant group was significantly lower than uterine fibroid ADC value of control group;CyclinD1, Rb, Sp1, Survivin, ER , ERβ, PRA and PRB protein expression in endometrial stromal sarcoma lesions of malignant group were significantly higher than those of control group while SULT1E1 protein expression was significantly lower than that of control group;CyclinD1, Rb, Sp1, Survivin, ER , ERβ, PRA and PRB protein expression in endometrial stromal sarcoma lesions of subgroup with low ADC value were significantly higher than those of subgroup with high ADC value while SULT1E1 protein expression was significantly lower than that of subgroup with high ADC value.Conclusion: Magnetic resonance diffusion weighted imaging ADC values can be used to evaluate the malignant biological behavior of endometrial stromal sarcoma.
基金funded by the Project of Occupational Health Risk Assessment and the National Occupational Health Standard Formulation of the National Institute of Occupational Health and Poison Control(Project No.102393220020090000020)National Key R&D Program of China(2022YFC2503205).
文摘What is already known about this topic?Lower extremity musculoskeletal diseases(LE-MSDs)have emerged as a significant contributor to the global disease economic burden and worker absenteeism,becoming a global public health concern.However,the epidemic characteristics of LE-MSDs among occupational populations in China are unknown.What is added by this report?This report finds that the LE-MSDs prevalence rate among key occupational groups in China is 17.7%,with the top 5 being toy manufacturing,medical personnel,automobile manufacturing,nonferrous metal smelting and rolling processing,and coal mining and washing.What are the implications for public health practice?This study investigated the occurrence of LE-MSDs in key industries in China and its possible risk factors to provide big data support for preventing and controlling such diseases in these industries.
基金supported by the grants from the Scientific Research Grant of Health and Family Planning Commission of Wuhan Municipality(No.WX19B06)the Health Commission of Hubei Province Scientific Research Project(No.WJ2019H429).
文摘To the Editor:Lumbar spinal canal stenosis(LSCS)is a common disease among older individuals.Hypertrophic lumbar ligamentum flavum(LLF)plays a vital role in the pathogenesis of LSCS.The physiological LLF is a kind of dense connective tissue with thickness of 2 to 4mm,which is mainly composed of fibroblasts and extracellular matrix(ECM).ECM contains elastic,collagen fibers and ground substances.The distribution characteristics of fibers show that a large number of elastic fibers are mixed with a small amount of the collagen fibers;mainly type I and type III collagen fibers.These fibers are arranged tightly and regularly along the direction of column’s axis.The LLF is located in the dorsal part of the dura mater of the spinal canal,and it attaches to the upper border and lower parts of anterior surfaces of the laminas.The hypotrophy of LLF is the consequence of several factors such as aging,inflammatory process,stress loading and genetic predisposition.
基金Supported by the National Natural Science Foundation of China(Intervention Mechanism of Yiqi Huatan Quyu Recipe on Pulmonary Vascular Remodeling of Chronic Obstructive Pulmonary Disease Based on Ca~(2+)-Calcineurin-NFAT Signaling Pathway,No.81403308)the National Natural Science Foundation of China(Based on"Lung Qi Deficiency and Missing Management and Regulation"Discuss the Mechanism of Chronic Obstructive Pulmonary Disease Pulmonary Vasoconstriction and KATP Channel,No.81373598)
文摘OBJECTIVE: To observe the effect of Qibai Pingfei capsule(QBPF) medicated serum on the proliferation of rat pulmonary arterial smooth muscle cells(PASMCs) under hypoxia conditions and to investigate its key molecular effects on the Ca^(2+)/calcineurin/nuclear factor of activated T-cells 3(NFATc3) signaling pathway.METHODS: QBPF was provided to rats via continuous gavage for 10 days. Primary rat PASMCs were cultured using the direct adherent culture method.Methyl thiazolyl tetrazolium assay was used to eval-uate the effect of QBPF on PASMCs proliferation under hypoxia conditions. Laser scanning confocal microscopy was used to detect changes in intracellular free calcium([Ca^(2+)]_i) in PASMC-loaded Fluo-3-AM.Real-time quantitative polymerase chain reaction and western blot were used to detect the transcription and protein expression levels of calcineurin and NFATc3 genes in PASMCs.RESULTS: Compared with normoxia conditions,PASMCs proliferated at 12, 24, 48, and 72 h under hypoxia conditions. QBPF at concentrations of 5%,10%, and 20% could inhibit hypoxia-induced PASMC proliferation to different degrees. The inhibitory effect was most significant in the 20% QBPF group under 24 h hypoxia conditions. The concentration of [Ca^(2+)]_iin PASMCs under hypoxia was increased and [Ca^(2+)]_iwas significantly decreased when co-incubated with QBPF at 5%, 10%, and 20%. Compared with normoxia conditions, the m RNA and protein expression levels of calcineurin and NFATc3 in PASMCs induced by hypoxia were up-regulated.QBPF application significantly down-regulated m RNA and protein expression levels of calcineurin and NFATc3 in PASMCs.CONCLUSION: QBPF can effectively inhibit hypoxia-induced proliferation of PASMCs through down-regulation of key molecular expression via the Ca^(2+)/calcineurin/NFATc3 pathway.
基金supported by the National Natural Science Foundation of China(No.81603670,81873169)the Hunan Provincial Natural Science Foundation of China(No.2017JJ3459,2020JJ4803)。
文摘Objective:Bushen Tiansui formula(BSTSF),a traditional Chinese medicine prescription,has been widely used to treat Alzheimer’s disease(AD).However,the mechanisms underlying its effects remain largely unknown.In this study,a rat AD model was used to study the effects of BSTSF on cognitive performance and expression of transfer RNA-derived small RNAs(tsRNAs)in the hippocampus,to determine whether treatment of AD with BSTSF could regulate the expression of tsRNAs,a novel small non-coding RNA.Methods:To generate a validated AD model,oligomeric amyloid-β_(1-42)(Aβ_(1-42))was injected intracerebroventricularly into rats.The Morris water maze(MWM)test was used to evaluate rat cognitive performance,and tsRNA-sequencing was conducted to examine tsRNA expression in the rat hippocampus.Potential targets were validated by quantitative real-time polymerase chain reaction(qRT-PCR).Bioinformatic analyses were conducted to investigate the biological function of candidate tsRNAs.Results:The learning and memory deficits of Aβ_(1-42)-induced AD rats,assessed by MWM tests,were clearly ameliorated by BSTSF treatment.A total of 387 tsRNAs were detected in the rat hippocampus.Among them,13 were significantly dysregulated in AD rats compared with sham control rats,while 57 were markedly altered by BSTSF treatment,relative to untreated AD rats(fold change>2 and P<0.05).Moreover,six BSTSF treatment-related tsRNAs were identified and validated by qRT-PCR.Bioinformatic analyses indicated that the six treatment-related tsRNAs had potential therapeutic roles,via multiple signaling pathways and Gene Ontology biological functions,including cyclic adenosine monophosphate and retrograde endocannabinoid signaling.Conclusion:This study identified a previously uncharacterized mechanism underlying the effects of BSTSF in alleviating the learning and memory deficits in Aβ_(1-42)-induced AD rats,demonstrating that tsRNAs are potential therapeutic targets of BSTSF in the treatment of AD.
基金financially supported by Hunan Provincial Key Laboratory of Micro&Nano Materials Interface Sciencethe National Natural Science Foundation of China(Nos.81603670,81873169,21773311,21972169)+1 种基金Hunan Provincial Natural Science Foundation of China(Nos.2017JJ3459,2020JJ4803)the Hunan Provincial Science and Technology Plan Project,China(No.2019TP1001)。
文摘Alzheimer’s disease(AD)is a progressive and fatal neurodegenerative condition and the most prevalent cause of dementia.This disease is characterized by progressive cognitive impairment.The prevalence of AD is currently affecting more than 35 million people and is rising worldwide.No efficient therapy is currently available due to low drug potency and a number of various obstacles to delivery.Recent nanotechnological advancements have the potential to offer promising therapeutic options.Progress on nanomaterials as well as their applications in biomedicine is receiving increasing attention,especially the advantages of nanomaterial-based drug delivery systems.The aim of this review is to comprehensively summarize the latest developments in nanomaterial-based strategies for AD treatment,including nanoparticles,liposomes and other options for the delivery of therapeutic compounds and scaffolds for cell delivery strategies.Future research directions are also proposed.We hope this review can provide important information to guide the future development of nanomaterials in AD treatment.
基金The Project of Occupational Health Risk Assessment and National Occupational Health Standard Formulation of National Institute of Occupational Health and Poison Control(Project No.131031109000150003).
文摘Summary What is already known about this topic?In recent decades,work-related musculoskeletal disorders(WMSDs)have become increasingly prominent and have become an important issue that is of universal concern and an urgent need to be solved in all countries of the world.What is added by this report?The top three industries or occupational groups with the highest standardized prevalence rate of WMSDs were flight attendants,medical staff,and vegetable greenhouses in that order.Women workers were 1.5 times more likely to suffer from WMSDs than men workers.
基金funded by the Project of Occupational Health Risk Assessment and National Occupational Health Standard Formulation of National Institute of Occupational Health and Poison Control(Project No.:131031109000150003).
文摘What is already known about this topic?Work-related musculoskeletal disorders(WMSDs)have a high prevalence and seriously harmful,which has attracted extensive attention in various countries in the world.Currently,the occurrence and rules of WMSDs in key industries are not known in China.What is added by this report?The prevalence of WMSDs is relatively high among professional populations in key industries in China,with the most commonly affected body parts concentrated in neck,shoulders,and low back and increasing with age and working years.What are the implications for public health practice?This study determined the prevalence and distribution characteristics of WMSDs in key industries in China and provided scientific evidence to recommend for inclusion of WMSDs in the new revision of the list of occupational diseases in China.
