Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial ...Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial biomedical media for the research and treatment of infectious diseases.Their applications in the realm of infectious diseases include pathogen detection,exploration of biological mechanisms,and development of anti-infective drugs.This review provides a concise introduction to the severity,classification,and pathogenesis of infectious diseases.Subsequently,we examined the distinctiveness and design strategies of functional probes for diagnosing and treating infectious diseases,shedding light on their design rationale using typical examples.We discuss the current status and challenges associated with the clinical implementation of functional probes.Furthermore,we explored the prospects of using these probes for the diagnosis and treatment of infectious diseases.This review aims to offer novel insights into the design of diagnostic probes for infectious diseases and broaden their applications in disease treatment.展开更多
Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue cultur...Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in vivo.To overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to therapy.With our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and proliferation.In addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK pathway.With our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.展开更多
Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to ...Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to overcome this challenge.The chip comprises a double spiral focusing unit,a flow resistance-based sample enrichment module with fine-tunable outlets,and a crossflow droplet generation unit.Utilizing a low-density cell/bead suspension(2×10^(6) objects/mL),cells/beads are focused into a near-equidistant linear arrangement within the double spiral microchannel.The excess water phase is diverted while cells/beads remain focused and sequentially encapsulated in individual droplets.Focusing performance was assessed through numerical simulations and experiments at three flow rates(40,60,80μL/min),demonstrating successful focusing at 40 and 80μL/min for beads and cells,respectively.In addition,both simulation and experimental results revealed that the flow resistance at the sample enrichment module is adjustable by punching different outlets,allowing over 50%of the aqueous phase to be removed.YOLOv8n-based droplet detection algorithms realized the counting of cells/beads in droplets,statistically demonstrating single-cell and bead encapsulation rates of 72.2%and 79.2%,respectively.All the results indicate that this on-chip sample enrichment approach can be further developed and employed as a critical component in single-cell encapsulation in water-in-oil droplets.展开更多
基金National Science Foundation for Excellent Young Project,Grant/Award Number:82322042National Key Research and Development Program of China,Grant/Award Number:2021YFC2302200+2 种基金National Natural Science Foundation of China,Grant/Award Number:82272248Training Project of National Science Foundation for Outstanding/Distinguished Young Scholars of Southern Medical University,Grant/Award Number:C620PF0217Science Fund for Distinguished Young Scholars of Guangdong Province,Grant/Award Number:2022B1515020089。
文摘Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial biomedical media for the research and treatment of infectious diseases.Their applications in the realm of infectious diseases include pathogen detection,exploration of biological mechanisms,and development of anti-infective drugs.This review provides a concise introduction to the severity,classification,and pathogenesis of infectious diseases.Subsequently,we examined the distinctiveness and design strategies of functional probes for diagnosing and treating infectious diseases,shedding light on their design rationale using typical examples.We discuss the current status and challenges associated with the clinical implementation of functional probes.Furthermore,we explored the prospects of using these probes for the diagnosis and treatment of infectious diseases.This review aims to offer novel insights into the design of diagnostic probes for infectious diseases and broaden their applications in disease treatment.
基金supported by NUS Presidential Young Professorship,MOE Tier 1 grantsupported by the NUS Research Scholarship.
文摘Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in vivo.To overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to therapy.With our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and proliferation.In addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK pathway.With our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.
文摘Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to overcome this challenge.The chip comprises a double spiral focusing unit,a flow resistance-based sample enrichment module with fine-tunable outlets,and a crossflow droplet generation unit.Utilizing a low-density cell/bead suspension(2×10^(6) objects/mL),cells/beads are focused into a near-equidistant linear arrangement within the double spiral microchannel.The excess water phase is diverted while cells/beads remain focused and sequentially encapsulated in individual droplets.Focusing performance was assessed through numerical simulations and experiments at three flow rates(40,60,80μL/min),demonstrating successful focusing at 40 and 80μL/min for beads and cells,respectively.In addition,both simulation and experimental results revealed that the flow resistance at the sample enrichment module is adjustable by punching different outlets,allowing over 50%of the aqueous phase to be removed.YOLOv8n-based droplet detection algorithms realized the counting of cells/beads in droplets,statistically demonstrating single-cell and bead encapsulation rates of 72.2%and 79.2%,respectively.All the results indicate that this on-chip sample enrichment approach can be further developed and employed as a critical component in single-cell encapsulation in water-in-oil droplets.
