AIM： To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS： Chitosan was chemically modified by gelatinizatio...AIM： To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS： Chitosan was chemically modified by gelatinization, and made into films of 60 μm in thickness, and sterilized. Two hundred Sprague-Dawley rats were randomly divided into five groups, Sham-operation group （group A）, wound-induced adhesion group （group B）, purified talc-induced adhesion group （group C）, vascular ligation-induced adhesion group （group D）, and infection-induced adhesion group （group E）, respectively. In each group, the rats were treated with different adhesion-inducing methods at the cecum of vermiform processes and then were divided into control and experimental subgroups. Serous membrane surface of vermiform processes were covered with the films in the experimental subgroups, and no films were used in the control subgroups. After 2 and 4 wk of treatments, the abdominal cavities were reopened and the adhesive severity was graded blindly according to Bhatia＇s method. The cecum of vermiform processes were resected for hydroxyproline （OHP） measurement and pathological examination. RESULTS： Adhesion severity and OHP level： After 2 and 4 wk of the treatments, in the experimental subgroups, the adhesions were significantly lighter and the OHP levels were significantly lower than those of the control subgroups in group B （2 wk： 0.199 ± 0.026 vs 0.285 ± 0.041 μg/mg pr, P 〈 0.001; 4 wk： 0.183 ± 0.034 vs 0.276 ± 0.03 μg/mg pr, P 〈 0.001）, D （2 wk： 0.216 ± 0.036 vs 0.274 ± 0.040 μg/mg pr, P = 0.004; 4 wk： 0.211 ± 0.044 vs 0.281 ± 0.047 μg/mg pr, P = 0.003） and E （2 wk： 0.259 ± 0.039 vs 0.371 ± 0.040 μg/mg pr, P 〈 0.001; 4 wk： 0.242 ± 0.045 vs 0.355 ± 0.029 μg/mg pr, P 〈 0.001）, but there were no significant differences in groups A （2wk： 0.141 ± 0.028 vs 0.137 =k 0.026 μg/mg pr, P = 0.737; 4 wk： 0.132 ± 0.031 vs 0.150 ± 0.035 μg/mg pr, P = 0.225） and C （2 wk： 0.395 ± 0.044 vs 0.378 ± 0.043 μg/mg pr, P = 0.387; 4 wk： 0.370 ± 0.032 vs 0.367 ± 0.041 μg/mg pr, P = 0.853）; Pathological changes： In group B, the main pathological changes were fibroplasias in the treated serous membrane surface and in group D, the fibroplasia was shown in the whole layer of the vermiform processes. In group E, the main pathological changes were acute and chronic suppurative inflammatory reactions. These changes were lighter in the experimental subgroups than those in the control subgroups in the three groups. In group C, the main changes were foreign body giant cell and granuloma reactions and fibroplasias in different degrees, with no apparent differences between the experimental and control subgroups. CONCLUSION： The gelatinizedly-modified chitosan film is effective on preventing peritoneal adhesions induced by wound, ischemia and infection, but the effect is not apparent in foreign body-induced adhesion.展开更多
Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay(ELISA),a hybridoma cell line strain secreting anti-HBs monoclonal antibody(mAb)(defined G6 mAb)was obtained.The cells grew and s...Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay(ELISA),a hybridoma cell line strain secreting anti-HBs monoclonal antibody(mAb)(defined G6 mAb)was obtained.The cells grew and secreted mAb stably.Antibody titers in the culture supernatant and ascites were 2.048�106 and 4.096�106,respectively.By applying the anti-HBs G6 mAb and horseradish peroxidase(HRP)-labeled goat anti-HBs antibody,we developed a sandwich ELISA(defined G6m ELISA)for detecting both wild-type and immune escape mutant HBsAgs(IEM HBsAg).The assay was performed to detect 17 species of genome recombinant expression HBsAg,including two wild-type species and 15 IEM HBsAg species,which varied in the“a”determinant,in a group of patients infected with hepatitis B virus(HBV).The patients previously had a lower ELISA detection signal[(absorbance of patients/absorbance of normal people(P/N):1.0–4.5)].The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125μg/L;12 of 15 IEM HBsAg species(P/N≥2.5)were positive for G6 mAb.Of the positive IEM HBsAg species,two had a low absorbance value at 450 nm(A450),one had an intermediate A450 value and nine had a high A450 value,which was 7.55%(mean),59.4%and 92.1%–109.4%of the wild-type A450 value,respectively.The two species with low A450 value and the three negative species mutated at the bases 120–124 in thefirst loop of the HBV“a”determinant.Using the G6 ELISA and two commercial ELISA kits(A and B),177 patients were tested.The G6 ELISA had a significantly higher detection rate than either commercial ELISAs(19.21%vs 14.89%and 6.21%,respectively;P<0.01,P<0.05,respectively).展开更多
A case of Sluder’s syndrome in a female patient,aged 56 years old,suffered from intermittent headache for over 20 years.The therapeutic methods include acupuncture with filiform needle mainly applied to the acupoints...A case of Sluder’s syndrome in a female patient,aged 56 years old,suffered from intermittent headache for over 20 years.The therapeutic methods include acupuncture with filiform needle mainly applied to the acupoints on governor vessel,foot-shaoyang meridian and foot-yangrning meridian,the external application of Wuzhuyu(吴茱萸Fructus Evodiae)on Yongquan(涌泉KI1),as well as the pricking and bleeding method with the three-edge needle on Touwei(头维ST8).The alliance of these three therapies co-act on promoting the circulation of shaoyang and yangming meridians and governor vessel and have obtained an satisfactory clinical effect.After treated for 5 weeks,the symptoms all disappeared completely,such as headache,vertigo and discomfort on eyes and they were not recurred in 1-year follow-up.展开更多
基金The National Natural Science Foundation of China, No. 50173023
文摘AIM： To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS： Chitosan was chemically modified by gelatinization, and made into films of 60 μm in thickness, and sterilized. Two hundred Sprague-Dawley rats were randomly divided into five groups, Sham-operation group （group A）, wound-induced adhesion group （group B）, purified talc-induced adhesion group （group C）, vascular ligation-induced adhesion group （group D）, and infection-induced adhesion group （group E）, respectively. In each group, the rats were treated with different adhesion-inducing methods at the cecum of vermiform processes and then were divided into control and experimental subgroups. Serous membrane surface of vermiform processes were covered with the films in the experimental subgroups, and no films were used in the control subgroups. After 2 and 4 wk of treatments, the abdominal cavities were reopened and the adhesive severity was graded blindly according to Bhatia＇s method. The cecum of vermiform processes were resected for hydroxyproline （OHP） measurement and pathological examination. RESULTS： Adhesion severity and OHP level： After 2 and 4 wk of the treatments, in the experimental subgroups, the adhesions were significantly lighter and the OHP levels were significantly lower than those of the control subgroups in group B （2 wk： 0.199 ± 0.026 vs 0.285 ± 0.041 μg/mg pr, P 〈 0.001; 4 wk： 0.183 ± 0.034 vs 0.276 ± 0.03 μg/mg pr, P 〈 0.001）, D （2 wk： 0.216 ± 0.036 vs 0.274 ± 0.040 μg/mg pr, P = 0.004; 4 wk： 0.211 ± 0.044 vs 0.281 ± 0.047 μg/mg pr, P = 0.003） and E （2 wk： 0.259 ± 0.039 vs 0.371 ± 0.040 μg/mg pr, P 〈 0.001; 4 wk： 0.242 ± 0.045 vs 0.355 ± 0.029 μg/mg pr, P 〈 0.001）, but there were no significant differences in groups A （2wk： 0.141 ± 0.028 vs 0.137 =k 0.026 μg/mg pr, P = 0.737; 4 wk： 0.132 ± 0.031 vs 0.150 ± 0.035 μg/mg pr, P = 0.225） and C （2 wk： 0.395 ± 0.044 vs 0.378 ± 0.043 μg/mg pr, P = 0.387; 4 wk： 0.370 ± 0.032 vs 0.367 ± 0.041 μg/mg pr, P = 0.853）; Pathological changes： In group B, the main pathological changes were fibroplasias in the treated serous membrane surface and in group D, the fibroplasia was shown in the whole layer of the vermiform processes. In group E, the main pathological changes were acute and chronic suppurative inflammatory reactions. These changes were lighter in the experimental subgroups than those in the control subgroups in the three groups. In group C, the main changes were foreign body giant cell and granuloma reactions and fibroplasias in different degrees, with no apparent differences between the experimental and control subgroups. CONCLUSION： The gelatinizedly-modified chitosan film is effective on preventing peritoneal adhesions induced by wound, ischemia and infection, but the effect is not apparent in foreign body-induced adhesion.
文摘Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay(ELISA),a hybridoma cell line strain secreting anti-HBs monoclonal antibody(mAb)(defined G6 mAb)was obtained.The cells grew and secreted mAb stably.Antibody titers in the culture supernatant and ascites were 2.048�106 and 4.096�106,respectively.By applying the anti-HBs G6 mAb and horseradish peroxidase(HRP)-labeled goat anti-HBs antibody,we developed a sandwich ELISA(defined G6m ELISA)for detecting both wild-type and immune escape mutant HBsAgs(IEM HBsAg).The assay was performed to detect 17 species of genome recombinant expression HBsAg,including two wild-type species and 15 IEM HBsAg species,which varied in the“a”determinant,in a group of patients infected with hepatitis B virus(HBV).The patients previously had a lower ELISA detection signal[(absorbance of patients/absorbance of normal people(P/N):1.0–4.5)].The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125μg/L;12 of 15 IEM HBsAg species(P/N≥2.5)were positive for G6 mAb.Of the positive IEM HBsAg species,two had a low absorbance value at 450 nm(A450),one had an intermediate A450 value and nine had a high A450 value,which was 7.55%(mean),59.4%and 92.1%–109.4%of the wild-type A450 value,respectively.The two species with low A450 value and the three negative species mutated at the bases 120–124 in thefirst loop of the HBV“a”determinant.Using the G6 ELISA and two commercial ELISA kits(A and B),177 patients were tested.The G6 ELISA had a significantly higher detection rate than either commercial ELISAs(19.21%vs 14.89%and 6.21%,respectively;P<0.01,P<0.05,respectively).
基金National natural science foundation of China:No.81674088Project of Shaanxi Administration Bureau of Traditional Chinese Medicine:No.2019-ZZ-JC023Youth Talent Support Plan of Shannxi University Science and Technology Association:No.20180308。
文摘A case of Sluder’s syndrome in a female patient,aged 56 years old,suffered from intermittent headache for over 20 years.The therapeutic methods include acupuncture with filiform needle mainly applied to the acupoints on governor vessel,foot-shaoyang meridian and foot-yangrning meridian,the external application of Wuzhuyu(吴茱萸Fructus Evodiae)on Yongquan(涌泉KI1),as well as the pricking and bleeding method with the three-edge needle on Touwei(头维ST8).The alliance of these three therapies co-act on promoting the circulation of shaoyang and yangming meridians and governor vessel and have obtained an satisfactory clinical effect.After treated for 5 weeks,the symptoms all disappeared completely,such as headache,vertigo and discomfort on eyes and they were not recurred in 1-year follow-up.
