Effects of Commiphora swynnertonii,Synadenium glaucescens and Allium sativum extracts on the tomato leaf miner,Tuta absoluta(Meyrick)were evaluated on the adults and in screen house conditions.The adult stage was invo...Effects of Commiphora swynnertonii,Synadenium glaucescens and Allium sativum extracts on the tomato leaf miner,Tuta absoluta(Meyrick)were evaluated on the adults and in screen house conditions.The adult stage was involved with 30 adults that were reared in an insectarium,the experimental design was a completely randomized design(plant extracts from three plants species×three doses of 2%,4% and 8%).In the screen house,experimental dispositive was a completely randomized block design(two varieties of tomatoes×three plants extracts).Larval counts were performed after 0,1,2,3 and 7 d of treatment,40 tomato leaves(10×4 replicates)were randomly taken from each treatment.The mean percentage mortality of adults was recorded daily for 5 d.Results indicated that,each plant extract caused significant mortality to adults of T.absoluta after 5 d in comparison to the control.Leaf dipping against adult of T.absoluta proved to be the most effective for all plant extracts at 30%-100%.Commiphora resulted in the adults’mortality of 100%.In the screen house Commiphora showed the high reduction of infestation for Tanya and Cal J varieties.Treatment with this plant extract resulted in the highest fruit yield and the lowest yield loss compared to all the plant extracts.C.swynnertonii extract is recommended into integrated pest management strategies for the control of T.absoluta.展开更多
Background:Taeniasis and cysticercosis are two diseases caused by Taenia solium,a parasite transmitted between humans and pigs,leading to considerable economic loss and disabilities.Transmission of the parasite is lin...Background:Taeniasis and cysticercosis are two diseases caused by Taenia solium,a parasite transmitted between humans and pigs,leading to considerable economic loss and disabilities.Transmission of the parasite is linked to environmental and behavioural factors such as inadequate sanitation and hygiene,poor pig management,and consumption of infected pork.This study used implementation research method to design a health education intervention strategy for reducing T.solium infections in Burkina Faso,a country endemic for the parasite.Methods:Eighteen group discussions were conducted with 8-18 participants each in three villages.In addition,structured interviews were conducted among 4777 participants and 2244 pig owners,who were selected through cluster random sampling in 60 villages of three provinces of Burkina Faso.Both approaches assessed knowledge and practices related to T.solium.The information obtained was used to develop a community-adapted health education intervention strategy to control taeniasis and cysticercosis in Burkina Faso.Results:The group discussions revealed that participants had a poor quality of life due to the diseases as well as inadequate access to latrines,safe water,and healthcare services.In addition,it was found that pig production was an important economic activity,especially for women.Furthermore,financial and knowledge constraints were important limitations to improved pig management and latrine construction.The survey data also showed that open defecation and drinking unboiled water were common behaviours,enhanced by a lack of knowledge regarding the transmission of the parasite,perceived financial barriers to the implementation of control measures,lack of public sensitization,as well as a lack of self-efficacy towards control of the parasite.Nevertheless,the perceived financial benefits of controlling porcine cysticercosis could be emphasized by an education program that discourages open defecation and encourages drinking safe water.The final intervention strategy included a Participatory Hygiene and Sanitation Transformation(PHAST)approach,as well as a 52-min film and an accompanying comic booklet.Conclusions:The main problem in the study communities regarding the transmission of T.solium cysticercosis is the random disposal of human faeces,which can be contaminated with parasite eggs.Prevention of open defecation requires the building of latrines,which can be quite problematic in economically challenged settings.Providing the community with the skills to construct durable latrines using low-cost locally available materials would likely help to resolve this problem.Further studies are required to implement and evaluate the T.solium control strategy developed in this study.展开更多
Pig production is becoming increasingly important in Mbozi and Mbeya rural districts to characterise smallholder pig Eastern Africa in recent years. Two studies were carried out in production systems and associated pi...Pig production is becoming increasingly important in Mbozi and Mbeya rural districts to characterise smallholder pig Eastern Africa in recent years. Two studies were carried out in production systems and associated pig management practises. A participatory rural appraisal （PRA）, involving 279 pigs-keeping households in nine villages and a cross sectional survey using a random sample of 300 pig farmers in 30 villages of the study districts, were used to collect data. Important economic activities were crop farming, livestock keeping, petty business, casual labour and artisan works in that order. The mean farm size and number of pigs per household was 2 ± 2.7 ha and 4.9 ±4.2 pigs, respectively. The largest categories of pig herd structure were breeding females followed by piglets. Age, marital status, household size and land size of household head had significant influence on the pig herd size （P 〈 0.05）. Pig farmers practised three main types of pig management systems, namely, total confinement, semi confinement and free range/herding. Each system had specific management practices in relation to feeding and housing. The frequencies of these systems varied significantly （P 〈 0.01） by socio-economic factors, such as, educational status of household head, land size and herd sizes of pigs, goats and cattle. The authors recommend effective use of locally available resources and careful consideration of existing farming systems for sustainable development of smallholder pig production.展开更多
Taenia solium cysticercosis is an emerging food borne parasitic zoonosis in urban centres where infected pigs from rural areas are slaughtered and consumed. The public is at risk of acquiring the infection if meat ins...Taenia solium cysticercosis is an emerging food borne parasitic zoonosis in urban centres where infected pigs from rural areas are slaughtered and consumed. The public is at risk of acquiring the infection if meat inspection and control is not effective. This study was conducted to establish the prevalence of porcine cysticercosis in pigs slaughtered in Dar es Salaam City and Morogoro Municipality and assessed the existence of risk factors for its transmission. Meat inspection was performed in pigs slaughtered in official slaughter slabs where direct observation and check-list based questions were used to assess the risk factors for disease transmission. The results revealed porcine cysticercosis prevalence of 6.3% and 1.5% in pigs slaughtered in Dares Salaam City and Morogoro Municipality, respectively. Possible risk factors for T. solium cysticercosis transmission included lack of centralized slaughtering facilities for pigs, inadequate meat inspectors, lack or inadequate meat inspection and control, poor knowledge among butchers and pork vendors about the parasite and possible public health implications. In addition, the findings indicated that the public was at high risk of acquiring the infection if immediate control measures were not taken. In view of this, it is recommended that the pig slaughtering should be centralized for effective inspection and pork control. Lastly, constant public health education should be provided to butchers, pork vendors and the public in general.展开更多
A study on nontuberculous mycobacteria （NTM） was carried out in wildlife-livestock interface of Katavi Rukwa ecosystem （KRE）. 328 livestock tissues and 178 wild animals were cultured, wild animals were sampled opp...A study on nontuberculous mycobacteria （NTM） was carried out in wildlife-livestock interface of Katavi Rukwa ecosystem （KRE）. 328 livestock tissues and 178 wild animals were cultured, wild animals were sampled opportunistically during professional hunting and game cropping operations in the KRE protected areas. The objective of the study was to generate data on epidemiology of NTM in the wildlife-livestock interface of the KRE. Methods used to identify the NTM were： culture and isolation, polymerase chain reaction, protein heat shock 65 kilodalton （hsp65） and sequencing. Mycobacteria were detected on 25.9% and 11.9% of livestock and wildlife tissue cultures, respectively. The most NTM isolated were M. kansasii （30%）, M. gastri （30%）, M. fortuitum （1%）, M. intracellulare （4%）, M. indicuspranii （4%）, M. nonchromogenicum （6%） and M. lentiflavum （6%）. Other NTM in smaller percentages were M. hibernae, M. engbaekii, M. septicum, M. arupense and 34.. godii. Due to rise of NTM infection in both human and animals, it is recommended that awareness and laboratory facilities be improved to curb the underreporting especially in TB-endemic countries. For species specific identification, a network of national and regional laboratories is promoted.展开更多
In order to investigate associations between individual calf parameters and/or herd management practices and dairy calf body weight (BW) attainment in smallholder farms in Tanzania, a prospective study was carried out...In order to investigate associations between individual calf parameters and/or herd management practices and dairy calf body weight (BW) attainment in smallholder farms in Tanzania, a prospective study was carried out at 121 farms in Mvomero and Njombe districts and included 156 crossbred dairy calves. Information on management factors and sources of calf drinking water were collected by personal observation and interview during four visits. At each visit, BW assessments were conducted by the same veterinarian and blood samples collected. The blood samples were analyzed for hematological parameters, total blood protein, glucose, and serum phosphorus and urea. Low BW was associated with smaller amount of milk fed to calves, early weaning, birth during the wet season, and well/tap water as the main water source (P < 0.05). Low blood total protein, high urea, low phosphorus and low hemoglobin concentrations were also associated with retarded growth. These findings identified and quantified some critical factors that can guide farmers in improving calf performance on smallholder dairy farms in Tanzania. To improve calf BW, the protein and mineral fraction of the roughage should be increased and water supply should be improved. In conclusion, appropriate changes in milk feeding routines and breeding management would also be expected to improve productivity.展开更多
Dairy cattle are frequently fed high-concentrate(HC)diets in modern intensive feeding systems,especially in the transition period.During this period,cows face many alterations that include hormonal changes and shiftin...Dairy cattle are frequently fed high-concentrate(HC)diets in modern intensive feeding systems,especially in the transition period.During this period,cows face many alterations that include hormonal changes and shifting to a lactating state.Switching to a HC diet that may disrupt the ruminal microbiota balance can lead to subacute ruminal acidosis(SARA).Moreover,the main factor shaping the rumen microbiota is dietary composition,especially the ratio of starch to fibrous carbohydrates.Feeding highly fermentable carbohydrate diets after adaptation to forage diets leads to a rumen fermentation rate that exceeds rumen absorption and buffering rates,resulting in a reduction in ruminal pH.As a result of Gram-negative bacterial cell lysis,an increase in harmful ruminal bacterial metabolites,including lipopolysaccharide,lactic acid,and histamine,is observed.The interactions between the host immune system and the ruminal microbiota play an essential role in many physiological processes and the development of the disorder.Progress in DNA sequencing and bioinformatics platforms provides new opportunities to investigate the composition of ruminal microbes and yields unique advances in understanding ecology of the rumen.Subacute ruminal acidosis is linked with a change in the ruminal microbiota structure and richness and with other metabolic disorders;such as rumenitis,milk fat depression,laminitis,and liver abscesses.Therefore,this review aims to explore a better understanding of the crosstalk between diet and microbiota in the prevalence of rumen acidosis and its consequences,which is crucial for control strategies such as feeding management,and supplementation with thiamine,prebiotics,and probiotics.展开更多
文摘Effects of Commiphora swynnertonii,Synadenium glaucescens and Allium sativum extracts on the tomato leaf miner,Tuta absoluta(Meyrick)were evaluated on the adults and in screen house conditions.The adult stage was involved with 30 adults that were reared in an insectarium,the experimental design was a completely randomized design(plant extracts from three plants species×three doses of 2%,4% and 8%).In the screen house,experimental dispositive was a completely randomized block design(two varieties of tomatoes×three plants extracts).Larval counts were performed after 0,1,2,3 and 7 d of treatment,40 tomato leaves(10×4 replicates)were randomly taken from each treatment.The mean percentage mortality of adults was recorded daily for 5 d.Results indicated that,each plant extract caused significant mortality to adults of T.absoluta after 5 d in comparison to the control.Leaf dipping against adult of T.absoluta proved to be the most effective for all plant extracts at 30%-100%.Commiphora resulted in the adults’mortality of 100%.In the screen house Commiphora showed the high reduction of infestation for Tanya and Cal J varieties.Treatment with this plant extract resulted in the highest fruit yield and the lowest yield loss compared to all the plant extracts.C.swynnertonii extract is recommended into integrated pest management strategies for the control of T.absoluta.
基金This work was conducted with support from the National Institute of Neurological Disorders and Stroke and the Fogarty International Center of the National Institutes of Health under the Brain Disorders in the Developing World:Research Across the Lifespan program,grant R01NS064901(http://www.fic.nih.gov/Programs/Pages/brain-disorders.aspx)The funders had no role in study design,data collection and analysis,interpretation of data,preparation of the paper,or decision to publish the data.
文摘Background:Taeniasis and cysticercosis are two diseases caused by Taenia solium,a parasite transmitted between humans and pigs,leading to considerable economic loss and disabilities.Transmission of the parasite is linked to environmental and behavioural factors such as inadequate sanitation and hygiene,poor pig management,and consumption of infected pork.This study used implementation research method to design a health education intervention strategy for reducing T.solium infections in Burkina Faso,a country endemic for the parasite.Methods:Eighteen group discussions were conducted with 8-18 participants each in three villages.In addition,structured interviews were conducted among 4777 participants and 2244 pig owners,who were selected through cluster random sampling in 60 villages of three provinces of Burkina Faso.Both approaches assessed knowledge and practices related to T.solium.The information obtained was used to develop a community-adapted health education intervention strategy to control taeniasis and cysticercosis in Burkina Faso.Results:The group discussions revealed that participants had a poor quality of life due to the diseases as well as inadequate access to latrines,safe water,and healthcare services.In addition,it was found that pig production was an important economic activity,especially for women.Furthermore,financial and knowledge constraints were important limitations to improved pig management and latrine construction.The survey data also showed that open defecation and drinking unboiled water were common behaviours,enhanced by a lack of knowledge regarding the transmission of the parasite,perceived financial barriers to the implementation of control measures,lack of public sensitization,as well as a lack of self-efficacy towards control of the parasite.Nevertheless,the perceived financial benefits of controlling porcine cysticercosis could be emphasized by an education program that discourages open defecation and encourages drinking safe water.The final intervention strategy included a Participatory Hygiene and Sanitation Transformation(PHAST)approach,as well as a 52-min film and an accompanying comic booklet.Conclusions:The main problem in the study communities regarding the transmission of T.solium cysticercosis is the random disposal of human faeces,which can be contaminated with parasite eggs.Prevention of open defecation requires the building of latrines,which can be quite problematic in economically challenged settings.Providing the community with the skills to construct durable latrines using low-cost locally available materials would likely help to resolve this problem.Further studies are required to implement and evaluate the T.solium control strategy developed in this study.
文摘Pig production is becoming increasingly important in Mbozi and Mbeya rural districts to characterise smallholder pig Eastern Africa in recent years. Two studies were carried out in production systems and associated pig management practises. A participatory rural appraisal （PRA）, involving 279 pigs-keeping households in nine villages and a cross sectional survey using a random sample of 300 pig farmers in 30 villages of the study districts, were used to collect data. Important economic activities were crop farming, livestock keeping, petty business, casual labour and artisan works in that order. The mean farm size and number of pigs per household was 2 ± 2.7 ha and 4.9 ±4.2 pigs, respectively. The largest categories of pig herd structure were breeding females followed by piglets. Age, marital status, household size and land size of household head had significant influence on the pig herd size （P 〈 0.05）. Pig farmers practised three main types of pig management systems, namely, total confinement, semi confinement and free range/herding. Each system had specific management practices in relation to feeding and housing. The frequencies of these systems varied significantly （P 〈 0.01） by socio-economic factors, such as, educational status of household head, land size and herd sizes of pigs, goats and cattle. The authors recommend effective use of locally available resources and careful consideration of existing farming systems for sustainable development of smallholder pig production.
文摘Taenia solium cysticercosis is an emerging food borne parasitic zoonosis in urban centres where infected pigs from rural areas are slaughtered and consumed. The public is at risk of acquiring the infection if meat inspection and control is not effective. This study was conducted to establish the prevalence of porcine cysticercosis in pigs slaughtered in Dar es Salaam City and Morogoro Municipality and assessed the existence of risk factors for its transmission. Meat inspection was performed in pigs slaughtered in official slaughter slabs where direct observation and check-list based questions were used to assess the risk factors for disease transmission. The results revealed porcine cysticercosis prevalence of 6.3% and 1.5% in pigs slaughtered in Dares Salaam City and Morogoro Municipality, respectively. Possible risk factors for T. solium cysticercosis transmission included lack of centralized slaughtering facilities for pigs, inadequate meat inspectors, lack or inadequate meat inspection and control, poor knowledge among butchers and pork vendors about the parasite and possible public health implications. In addition, the findings indicated that the public was at high risk of acquiring the infection if immediate control measures were not taken. In view of this, it is recommended that the pig slaughtering should be centralized for effective inspection and pork control. Lastly, constant public health education should be provided to butchers, pork vendors and the public in general.
文摘A study on nontuberculous mycobacteria （NTM） was carried out in wildlife-livestock interface of Katavi Rukwa ecosystem （KRE）. 328 livestock tissues and 178 wild animals were cultured, wild animals were sampled opportunistically during professional hunting and game cropping operations in the KRE protected areas. The objective of the study was to generate data on epidemiology of NTM in the wildlife-livestock interface of the KRE. Methods used to identify the NTM were： culture and isolation, polymerase chain reaction, protein heat shock 65 kilodalton （hsp65） and sequencing. Mycobacteria were detected on 25.9% and 11.9% of livestock and wildlife tissue cultures, respectively. The most NTM isolated were M. kansasii （30%）, M. gastri （30%）, M. fortuitum （1%）, M. intracellulare （4%）, M. indicuspranii （4%）, M. nonchromogenicum （6%） and M. lentiflavum （6%）. Other NTM in smaller percentages were M. hibernae, M. engbaekii, M. septicum, M. arupense and 34.. godii. Due to rise of NTM infection in both human and animals, it is recommended that awareness and laboratory facilities be improved to curb the underreporting especially in TB-endemic countries. For species specific identification, a network of national and regional laboratories is promoted.
基金the Norwegian Government through the PANTIL project at Sokoine University of Agriculture
文摘In order to investigate associations between individual calf parameters and/or herd management practices and dairy calf body weight (BW) attainment in smallholder farms in Tanzania, a prospective study was carried out at 121 farms in Mvomero and Njombe districts and included 156 crossbred dairy calves. Information on management factors and sources of calf drinking water were collected by personal observation and interview during four visits. At each visit, BW assessments were conducted by the same veterinarian and blood samples collected. The blood samples were analyzed for hematological parameters, total blood protein, glucose, and serum phosphorus and urea. Low BW was associated with smaller amount of milk fed to calves, early weaning, birth during the wet season, and well/tap water as the main water source (P < 0.05). Low blood total protein, high urea, low phosphorus and low hemoglobin concentrations were also associated with retarded growth. These findings identified and quantified some critical factors that can guide farmers in improving calf performance on smallholder dairy farms in Tanzania. To improve calf BW, the protein and mineral fraction of the roughage should be increased and water supply should be improved. In conclusion, appropriate changes in milk feeding routines and breeding management would also be expected to improve productivity.
基金the National Natural Science Foundation of China(NSFC No.31872988No.31572429)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PADA).
文摘Dairy cattle are frequently fed high-concentrate(HC)diets in modern intensive feeding systems,especially in the transition period.During this period,cows face many alterations that include hormonal changes and shifting to a lactating state.Switching to a HC diet that may disrupt the ruminal microbiota balance can lead to subacute ruminal acidosis(SARA).Moreover,the main factor shaping the rumen microbiota is dietary composition,especially the ratio of starch to fibrous carbohydrates.Feeding highly fermentable carbohydrate diets after adaptation to forage diets leads to a rumen fermentation rate that exceeds rumen absorption and buffering rates,resulting in a reduction in ruminal pH.As a result of Gram-negative bacterial cell lysis,an increase in harmful ruminal bacterial metabolites,including lipopolysaccharide,lactic acid,and histamine,is observed.The interactions between the host immune system and the ruminal microbiota play an essential role in many physiological processes and the development of the disorder.Progress in DNA sequencing and bioinformatics platforms provides new opportunities to investigate the composition of ruminal microbes and yields unique advances in understanding ecology of the rumen.Subacute ruminal acidosis is linked with a change in the ruminal microbiota structure and richness and with other metabolic disorders;such as rumenitis,milk fat depression,laminitis,and liver abscesses.Therefore,this review aims to explore a better understanding of the crosstalk between diet and microbiota in the prevalence of rumen acidosis and its consequences,which is crucial for control strategies such as feeding management,and supplementation with thiamine,prebiotics,and probiotics.
