Hirschsprung's disease(HSCR) or congenital megacolon is one of the differential diagnoses of chronic constipation mostly in infancy and may indeed represent a challenge for pediatricians, pediatric surgeons, and p...Hirschsprung's disease(HSCR) or congenital megacolon is one of the differential diagnoses of chronic constipation mostly in infancy and may indeed represent a challenge for pediatricians, pediatric surgeons, and pediatric pathologists. The diagnosis relies clearly on the identification of the absence of ganglion cells at the plexuses(submucosus and myentericus) of the bowel wall. HSCR is usually located at the terminal(distal) rectum with potential pre-terminal or proximal extension to the less distal large bowel(sigmoid colon). Astonishingly, there is some evidence that Hindu surgeons of prehistoric India may have been exposed and had considerable knowledge about HSCR, but this disease is notoriously and eponymously named to Dr. Harald Hirschsprung(1830-1916), who brilliantly presented two infants with fatal constipation at the Berlin conference of the German Society of Pediatrics more than one century ago. Historical milestones and diagnosis of HSCR(originally called "Die Hirschsprungsche Krankheit") are reviewed. More than 100 years following his meticulous and broad description, HSCR is still a puzzling disease for both diagnosis and treatment. HSCR remains a critical area of clinical pediatrics and pediatric surgery and an intense area of investigation for both molecular and developmental biologists.展开更多
[ Objective] To express lactate dehydrogenase C （LDH-C） gene in prokaryotic system and then purify the recombinant protein. E Meth- od] The LDI-I-C gene was amplified from black-lipped pika（ Ochotona curzoniae） by...[ Objective] To express lactate dehydrogenase C （LDH-C） gene in prokaryotic system and then purify the recombinant protein. E Meth- od] The LDI-I-C gene was amplified from black-lipped pika（ Ochotona curzoniae） by RT-PCR and ligated to the expression vector pET-32a. The E. coli BL21 （DE3） carrying the LDI-I-C gene was obtained and induced by IPTG （isopropy-beta-D-thiogalactoside）. The expression products were analyzed by SDS-PAGE and purified by affinity chromatography. L Result] An approximately 1.0 kbp band appeared in the RT-PCR products as the- oretically expected. As evidenced by PCR and double enzyme digestion, an approximately 1.0 kbp fragment appeared, which indicated successful construction of expression vector. As analyzed by SDS-PAGE, a fusion protein with molecular weight slightly larger than 45 kDa was expressed in form of inclusion body. And very pure fusion protein was obtained by nickel ions-charged affinity chromatography. [ Coaclusion] The LDH-C gene of black-lipped pika has been cloned and expressed in E. coil展开更多
Background:In patients receiving anticancer chemotherapy,polyethylene glycolated recombinant human granulocyte-colony stimulating factor(PEG-rhG-CSF)was used for prophylaxis of chemotherapy-induced neutropenia.However...Background:In patients receiving anticancer chemotherapy,polyethylene glycolated recombinant human granulocyte-colony stimulating factor(PEG-rhG-CSF)was used for prophylaxis of chemotherapy-induced neutropenia.However,the side effect of PEG-rhG-CSF use on fasting blood glucose(FBG)level remains unclear.Materials and Methods:Cancer patients receiving chemotherapy and PEG-rhG-CSF were enrolled in our study.Baseline glucose(Glucose 1)was measured before PEG-rhG-CSF use,a second FBG test(Glucose 2)was performed after PEG-rhG-CSF use.Mean glucose levels were compared using t test.Results:The time interval between PEG-rhG-CSF use and the second glucose test was 2.4±1.5 days.The mean Glucose 1 was 5.18±0.53 mmol/L,and Glucose 2 was 3.80±1.13 mmol/L.Statistical analysis showed a significant difference between Glucose 1 and 2 existed(P<0.001).Conclusion:Our study identifies a hypoglycemic side effect of PEG-rhG-CSF occurs in cancer patients undergoing anti-cancer chemotherapy.Our results highlight the caution required when using PEG-rhG-CSF for prophylaxis of chemotherapyinduced neutropenia.展开更多
Background： It was believed that inflammatory response induced by cardiopulmonary bypass （CPB） was blamed for complications after cardiac surgery. To improve the outcome, many pharmacological interventions have bee...Background： It was believed that inflammatory response induced by cardiopulmonary bypass （CPB） was blamed for complications after cardiac surgery. To improve the outcome, many pharmacological interventions have been applied to attenuate inflammatory response during CPB. The objective of this study was to investigate the effect of ulinastatin （urinary trypsin inhibitor [UTI]） on outcome after CPB surgery. Methods： Totally, 208 patients undergoing elective valves replacement between November 2013 and September 2014 were divided into Group U （n = 70） and Group C （n = 138） based on they received UTI or not. Categorical variables were compared between groups using Fisher＇s exact test, and continuous variables using unpaired Student＇s t-test or Mann-Whitney U-test. One-way analysis of variance and Dunnett＇s or Tukey＇s tests were used to compare values at different time points within the same group, The risk of outcomes was estimated and adjusted by multivariable logistic regression, propensity scoring, and mixed-effect models for all measured variables. Results： Both the serious complications in total, including death, acute lung injury, acute respiratory distress syndrome and acute kidney injury, and the other complications, including hemodialysis, infection, re-incubation, and tracheotomy were similar between the two groups （P 〉 0.05）. After adjusted by multivariable logistic regression and the propensity score, UTI still cannot be found any benefit to improve any outcomes after cardiac surgery. Also, no statistical differences with regard to duration of postoperative mechanical ventilation, the length of Intensive Care Unit and hospital stays （P 〉 0.05）. Conclusion： UTI did not improve postoperative outcomes in our patients after cardiopulmonary bypass surgery.展开更多
Primary biliary cholangitis(PBC) is an autoimmune disease involving dysregulation of a broad array of homeostatic and metabolic processes. Although considerable single-nucleotide polymorphisms have been unveiled, a la...Primary biliary cholangitis(PBC) is an autoimmune disease involving dysregulation of a broad array of homeostatic and metabolic processes. Although considerable single-nucleotide polymorphisms have been unveiled, a large fraction of risk factors remains enigmatic. Candidate genes with rare mutations that tend to confer more deleterious effects need to be identified. To help pinpoint cellular and developmental mechanisms beyond common noncoding variants, we integrate whole exome sequencing with integrative network analysis to investigate genes harboring de novo mutations. Prominent convergence has been revealed on a network of disease-specific co-expression comprised of 55 genes associated with homeostasis and metabolism. The transcription factor gene MEF2 D and the DNA repair gene PARP2 are highlighted as hub genes and identified to be up-and down-regulated, respectively, in peripheral blood data set. Enrichment analysis demonstrates that altered expression of MEF2 D and PARP2 may trigger a series of molecular and cellular processes with pivotal roles in PBC pathophysiology. Our study identifies genes with de novo mutations in PBC and suggests that a subset of genes in homeostasis and metabolism tend to act in synergy through converging on co-expression network, providing novel insights into the etiology of PBC and expanding the pool of molecular candidates for discovering clinically actionable biomarkers.展开更多
文摘Hirschsprung's disease(HSCR) or congenital megacolon is one of the differential diagnoses of chronic constipation mostly in infancy and may indeed represent a challenge for pediatricians, pediatric surgeons, and pediatric pathologists. The diagnosis relies clearly on the identification of the absence of ganglion cells at the plexuses(submucosus and myentericus) of the bowel wall. HSCR is usually located at the terminal(distal) rectum with potential pre-terminal or proximal extension to the less distal large bowel(sigmoid colon). Astonishingly, there is some evidence that Hindu surgeons of prehistoric India may have been exposed and had considerable knowledge about HSCR, but this disease is notoriously and eponymously named to Dr. Harald Hirschsprung(1830-1916), who brilliantly presented two infants with fatal constipation at the Berlin conference of the German Society of Pediatrics more than one century ago. Historical milestones and diagnosis of HSCR(originally called "Die Hirschsprungsche Krankheit") are reviewed. More than 100 years following his meticulous and broad description, HSCR is still a puzzling disease for both diagnosis and treatment. HSCR remains a critical area of clinical pediatrics and pediatric surgery and an intense area of investigation for both molecular and developmental biologists.
基金funded by the Applied Fundamental Study of Sichuan Province ( 2008JY0068)China Natural Science Foundation ( 31071700)Funds of the State Ethnic Affairs Commission of PRC ( 08XN04)
文摘[ Objective] To express lactate dehydrogenase C （LDH-C） gene in prokaryotic system and then purify the recombinant protein. E Meth- od] The LDI-I-C gene was amplified from black-lipped pika（ Ochotona curzoniae） by RT-PCR and ligated to the expression vector pET-32a. The E. coli BL21 （DE3） carrying the LDI-I-C gene was obtained and induced by IPTG （isopropy-beta-D-thiogalactoside）. The expression products were analyzed by SDS-PAGE and purified by affinity chromatography. L Result] An approximately 1.0 kbp band appeared in the RT-PCR products as the- oretically expected. As evidenced by PCR and double enzyme digestion, an approximately 1.0 kbp fragment appeared, which indicated successful construction of expression vector. As analyzed by SDS-PAGE, a fusion protein with molecular weight slightly larger than 45 kDa was expressed in form of inclusion body. And very pure fusion protein was obtained by nickel ions-charged affinity chromatography. [ Coaclusion] The LDH-C gene of black-lipped pika has been cloned and expressed in E. coil
文摘Background:In patients receiving anticancer chemotherapy,polyethylene glycolated recombinant human granulocyte-colony stimulating factor(PEG-rhG-CSF)was used for prophylaxis of chemotherapy-induced neutropenia.However,the side effect of PEG-rhG-CSF use on fasting blood glucose(FBG)level remains unclear.Materials and Methods:Cancer patients receiving chemotherapy and PEG-rhG-CSF were enrolled in our study.Baseline glucose(Glucose 1)was measured before PEG-rhG-CSF use,a second FBG test(Glucose 2)was performed after PEG-rhG-CSF use.Mean glucose levels were compared using t test.Results:The time interval between PEG-rhG-CSF use and the second glucose test was 2.4±1.5 days.The mean Glucose 1 was 5.18±0.53 mmol/L,and Glucose 2 was 3.80±1.13 mmol/L.Statistical analysis showed a significant difference between Glucose 1 and 2 existed(P<0.001).Conclusion:Our study identifies a hypoglycemic side effect of PEG-rhG-CSF occurs in cancer patients undergoing anti-cancer chemotherapy.Our results highlight the caution required when using PEG-rhG-CSF for prophylaxis of chemotherapyinduced neutropenia.
基金Financial support and sponsorship This work was supported by the National Natural Science Foundation of China （No. 81270324）.
文摘Background： It was believed that inflammatory response induced by cardiopulmonary bypass （CPB） was blamed for complications after cardiac surgery. To improve the outcome, many pharmacological interventions have been applied to attenuate inflammatory response during CPB. The objective of this study was to investigate the effect of ulinastatin （urinary trypsin inhibitor [UTI]） on outcome after CPB surgery. Methods： Totally, 208 patients undergoing elective valves replacement between November 2013 and September 2014 were divided into Group U （n = 70） and Group C （n = 138） based on they received UTI or not. Categorical variables were compared between groups using Fisher＇s exact test, and continuous variables using unpaired Student＇s t-test or Mann-Whitney U-test. One-way analysis of variance and Dunnett＇s or Tukey＇s tests were used to compare values at different time points within the same group, The risk of outcomes was estimated and adjusted by multivariable logistic regression, propensity scoring, and mixed-effect models for all measured variables. Results： Both the serious complications in total, including death, acute lung injury, acute respiratory distress syndrome and acute kidney injury, and the other complications, including hemodialysis, infection, re-incubation, and tracheotomy were similar between the two groups （P 〉 0.05）. After adjusted by multivariable logistic regression and the propensity score, UTI still cannot be found any benefit to improve any outcomes after cardiac surgery. Also, no statistical differences with regard to duration of postoperative mechanical ventilation, the length of Intensive Care Unit and hospital stays （P 〉 0.05）. Conclusion： UTI did not improve postoperative outcomes in our patients after cardiopulmonary bypass surgery.
基金supported in part by grants from the National Natural Science Foundation of China (81870397 to X.D.L.81620108002, 81771732, 81830016 to X.M+2 种基金and 81570469 to R.Q.T.)by grants from Jiangsu provincial research fund (BE2017713 to X.D.L and BL2018657 to Y.T.)a grant from National Key R&D Program of China (2016YFC0900400)。
文摘Primary biliary cholangitis(PBC) is an autoimmune disease involving dysregulation of a broad array of homeostatic and metabolic processes. Although considerable single-nucleotide polymorphisms have been unveiled, a large fraction of risk factors remains enigmatic. Candidate genes with rare mutations that tend to confer more deleterious effects need to be identified. To help pinpoint cellular and developmental mechanisms beyond common noncoding variants, we integrate whole exome sequencing with integrative network analysis to investigate genes harboring de novo mutations. Prominent convergence has been revealed on a network of disease-specific co-expression comprised of 55 genes associated with homeostasis and metabolism. The transcription factor gene MEF2 D and the DNA repair gene PARP2 are highlighted as hub genes and identified to be up-and down-regulated, respectively, in peripheral blood data set. Enrichment analysis demonstrates that altered expression of MEF2 D and PARP2 may trigger a series of molecular and cellular processes with pivotal roles in PBC pathophysiology. Our study identifies genes with de novo mutations in PBC and suggests that a subset of genes in homeostasis and metabolism tend to act in synergy through converging on co-expression network, providing novel insights into the etiology of PBC and expanding the pool of molecular candidates for discovering clinically actionable biomarkers.
