Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patie...Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patient with American tegumentary leishmaniasis in Mendoza,Argentina,a non-endemic region.Patient concerns:A 43-year-old male was admitted to a tertiary care hospital in Mendoza,Argentina Republic with a history of progressive nasal discharge,septal perforation,facial pain,and pruritus.Despite treatment for presumed nasal myiasis and vasculitis with granulomatosis,symptoms persisted.Diagnosis:American tegumentary leishmaniasis.Interventions:Intravenous liposomal amphotericin B.Outcomes:Follow-up at 30 days showed no recurrence of symptoms with a remarkable clinical improvement of the nasal lesion.Lessons:This case sheds light on the necessity of accurate identification for timely intervention and the need to recognize the diverse manifestations of American tegumentary leishmaniasis to avoid misdiagnosis.展开更多
The Antarctic Paleogene marine fossil record has been the key to reconstructing the evolution of the Weddellian Sea and final dismemberment of Southern Gondwana.In this context,Eocene marine vertebrates from Seymour(M...The Antarctic Paleogene marine fossil record has been the key to reconstructing the evolution of the Weddellian Sea and final dismemberment of Southern Gondwana.In this context,Eocene marine vertebrates from Seymour(Marambio)Island have provided valuable information.We present the first Eocene record of marine reptiles from the southern Atlantic Coast of South America.This corresponds to several postcranial turtle remains represented by a proximal end of the right humerus,three caudal and one thoracic vertebrae,a fragment of the left pubis,and ten ossicles of the dorsal carapace,coming from the Leticia Formation(late-mid Eocene)at Cabo Tiburones,Tierra del Fuego,Argentina.These materials show severalfeatures such as the size and general morphology of the humerus and vertebrae,and the presence of relatively small,irregular,smooth,and unkeeled ossicles,which allow us to assign them to Dermochelyidae indet.Dermochelyids are a cosmopolitan group ofcryptodiran turtles,registered from the late Cretaceous up to the recent,with some physiological-biological peculiarities(e.g.,endothermy and an exclusive jellyfish-based diet)and characterized by the presence of an osseous carapace formed by ossicles.The new finding from the Leticia Formation is an addition to the scarce and extremely fragmentary record of Eocene dermochelyids from the southern seas like those from the La Meseta and Submeseta formations(Antarctica)and the Waihao and Burnside formations(New Zealand).This new information allows us to discuss the presence of these turtles in such high latitudes in the past and its implication in the evolution of the Weddellian fauna.展开更多
In this work,we report long-term trends in the abundance and breeding performance of Adélie penguins(Pygoscelis adeliae)nesting in three Antarctic colonies(i.e.,at Martin Point,South Orkneys Islands;Stranger Poin...In this work,we report long-term trends in the abundance and breeding performance of Adélie penguins(Pygoscelis adeliae)nesting in three Antarctic colonies(i.e.,at Martin Point,South Orkneys Islands;Stranger Point/Cabo Funes,South Shetland Islands;and Esperanza/Hope Bay in the Antarctic Peninsula)from 1995/96 to 2022/23.Using yearly count data of breeding groups selected,we observed a decline in the number of breeding pairs and chicks in crèche at all colonies studied.However,the magnitude of change was higher at Stranger Point than that in the remaining colonies.Moreover,the index of breeding success,which was calculated as the ratio of chicks in crèche to breeding pairs,exhibited no apparent trend throughout the study period.However,it displayed greater variability at Martin Point compared to the other two colonies under investigation.Although the number of chicks in crèche of Adélie penguins showed a declining pattern,the average breeding performance was similar to that reported in gentoo penguin colonies,specifically,those undergoing a population increase(even in sympatric colonies facing similar local conditions).Consequently,it is plausible to assume a reduction of the over-winter survival as a likely cause of the declining trend observed,at least in the Stranger Point and Esperanza colonies.However,we cannot rule out local effects during the breeding season affecting the Adélie population of Martin Point.展开更多
The worldwide record of Cretaceous Neornithes and Neornithine-like birds is both controversial and poorly understood.However,in recent years,the Antarctic continent has yielded a substantial number of Maastrichtian av...The worldwide record of Cretaceous Neornithes and Neornithine-like birds is both controversial and poorly understood.However,in recent years,the Antarctic continent has yielded a substantial number of Maastrichtian avian specimens,contributing significantly to our understanding of the early evolution of this group.Simultaneously,the keen interest and collaborative efforts of various paleornithologists have resulted in a wealth of knowledge,which we have thoroughly reviewed,updated,and discussed in the context of recent discoveries.展开更多
In coronavirus disease 2019(COVID-19),severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)primarily targets the respiratory system,but evidence suggests extrapulmonary organ involvement,notably in the liver.Vir...In coronavirus disease 2019(COVID-19),severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)primarily targets the respiratory system,but evidence suggests extrapulmonary organ involvement,notably in the liver.Viral RNA has been detected in hepatic tissues,and in situ hybridization revealed virions in blood vessels and endothelial cells.Electron microscopy confirmed viral particles in hepatocytes,emphasizing the need for understanding hepatotropism and direct cytopathic effects in COVID-19-related liver injury.Various factors contribute to liver injury,including direct cytotoxicity,vascular changes,inflammatory responses,immune reactions from COVID-19 and vaccinations,and druginduced liver injury.Although a typical hepatitis presentation is not widely documented,elevated liver biochemical markers are common in hospitalized COVID-19 patients,primarily showing a hepatocellular pattern of elevation.Long-term studies suggest progressive cholestasis may affect 20%of patients with chronic liver disease post-SARS-CoV-2 infection.The molecular mechanisms underlying SARS-CoV-2 infection in the liver and the resulting liver damage are complex.This“Editorial”highlights the expression of the Angiotensin-converting enzyme-2 receptor in liver cells,the role of inflammatory responses,the impact of hypoxia,the involvement of the liver's vascular system,the infection of bile duct epithelial cells,the activation of hepatic stellate cells,and the contribution of monocyte-derived macrophages.It also mentions that pre-existing liver conditions can worsen the outcomes of COVID-19.Understanding the interaction of SARS-CoV-2 with the liver is still evolving,and further research is required.展开更多
In recent years,the taxonomy and systematics of the cartilaginous fish taxa from the Eocene La Meseta Formation of Seymour(Marambio)Island,Antarctica have been extensively discussed in a series of papers,resulting in ...In recent years,the taxonomy and systematics of the cartilaginous fish taxa from the Eocene La Meseta Formation of Seymour(Marambio)Island,Antarctica have been extensively discussed in a series of papers,resulting in a complete revision of the Antarctic Eocene ichthyofauna housed in the Vertebrate Paleontology collection of the Museo de La Plata,Argentina.This collection constitutes one of the largest and taxonomically most diverse in the world,with approximately 20000 specimens,which provides a solid database used for the analysis of qualitative and quantitative chondrichthyan taxonomic composition.The information provided herein might be useful to understand the sudden decline in chondrichthyan taxonomic diversity towards the top of La Meseta Formation as well as its potential relationship with environmental changes during the Eocene.展开更多
The first dinosaur discovered in the Antarctic continent was the ankylosaur Antarctopelta oliveroi in the 1980s.Nevertheless,since then several hypotheses of phylogenetical relationships have been proposed because the...The first dinosaur discovered in the Antarctic continent was the ankylosaur Antarctopelta oliveroi in the 1980s.Nevertheless,since then several hypotheses of phylogenetical relationships have been proposed because these have been depended on how the skeletal remains have been interpreted.The main obstacle for clarifying its phylogenetic position is that many portions of the skeleton remain unknown,in addition to the presence of unknown characters in typical ankylosaurs.Considered an ankylosaurid,nodosaurid,or even a chimaera,a recent proposal based on mostly complete material of a new ankylosaur from Chilean Patagonia provided support for a novel phylogenetic hypothesis:Antarctopelta and other southern ankylosaurs are an early branching clade,the Parankylosauria,whose origin probably dates to the Late Jurassic.In the light of this new view,a redescription of the available skeletal remains is provided together with a new reconstruction of the first Antarctic dinosaur known to the science community.展开更多
In tropical and subtropical forest ecosystems,cavities formed by decay processes are a key but scarce resource for birds that nest and roost in them,which makes them a highly sensitive group to logging.The piedmont fo...In tropical and subtropical forest ecosystems,cavities formed by decay processes are a key but scarce resource for birds that nest and roost in them,which makes them a highly sensitive group to logging.The piedmont forest of northwestern Argentina is a complex ecosystem with 113 tree and 120 bird species.It has high logging pressure on the few,well-conserved forest remnants,complicating the delineation of sustainable management guidelines for each tree or bird species in a short time.Our objective was to reduce the complexity of subtropical forests by grouping tree species according to the characteristics used by secondary cavity-nesting birds(i.e.non excavators).In the piedmont forest,50 plots of 0.25 ha were sampled to record cavity trees and cavity characteristics.These were then used in a cluster analysis to form tree groups.Additionally,cavities were searched to identify the bird species using the decay-formed cavities.A total of 187 cavity trees,comprising 23 tree species,were recorded,and these formed four tree groups or clusters.We recorded 86 cavities that were used by secondary cavitynesting bird species.The four tree groups were unequally used by secondary cavity nesters.The tree group that included valuable timber species(Myroxylon peruiferum,Anadenanthera colubrina and Calycophyllum multiflorum)and had the greatest cavity availability represented 71%of total cavity use.Another tree group with valuable timber species(Cedrela balansae and Amburana cearensis),measured>73 cm DBH and>21 m tall,had cavity entrances>0.10 cm2,and contributed 14%of all cavity use by birds.A third group had no highly economically valuable tree species,and included the snag category(i.e.standing dead trees)as well as a 15%of cavity use.The fourth tree group had a DBH<0.40 cm,only one highly economically valuable tree species(Cordia trichotoma),and supported no cavity use.The clustering of subtropical trees can reduce the complexity of these forests,hence easing their management by focusing on those groups with tree species showing similar characteristics and providing suitable nesting sites for secondary cavity-nesting birds.展开更多
<span style="font-family:""><span style="font-family:Verdana;">Infection with </span><i><span style="font-family:Verdana;">Toxoplasma gondii</span>...<span style="font-family:""><span style="font-family:Verdana;">Infection with </span><i><span style="font-family:Verdana;">Toxoplasma gondii</span></i><span style="font-family:Verdana;">, is one of the most widespread zoonoses in the world. Congenital Toxoplasmosis (CT) is particularly risky due to its fetal </span><span style="font-family:Verdana;">complications. Sulfadiazine (SDZ) and Pyrimethamine (PYR) are usually </span><span style="font-family:Verdana;">used </span><span style="font-family:Verdana;">for CT treatment in Argentina, to prevent morbidity. Due to the lack of </span><span style="font-family:Verdana;">commercial pediatric formulations, these must be prepared in the hospital pharmacy. This is the first report of serum concentrations measures in pediatric CT therapy for this combination of drugs. A bioanalytical method was developed for identification and simultaneous quantification of SDZ and PYR by High Performance Liquid Chromatography (HPLC) with UV detection. The validated method was applied to residual serum samples obtained from 6 pediatric patients undergoing treatment with SDZ 42.20 a 93.70 mg/kg/day and </span><span style="font-family:Verdana;">PYR 0.77 a 2.70 mg/kg/day. Sample pretreatment consisted </span></span><span style="font-family:Verdana;">on</span><span style="font-family:Verdana;"> a</span><span style="font-family:""><span style="font-family:Verdana;"> deproteini</span><span style="font-family:Verdana;">zation step followed by centrifugation and then injection of supernatant.</span><span style="font-family:Verdana;"> Limit of Detection (LOD) and Quantification (LOQ) were (0.17 ± 0.02 and 0.13 ± 0.02) μg/mL and (0.46 ± 0.01 and 0.36 ± 0.01) μg/mL for SDZ and PYR respectively, with an appropriate linear range. Concentrations range found </span><span style="font-family:Verdana;">were (<LOD</span></span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">162.04 ± 0.02) μg/mL for SDZ and (<LOD</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">7.30 ± 0.03) </span><span style="font-family:""><span style="font-family:Verdana;">μg/mL for PYR. We developed and validated in real pediatric samples, an acute, pre</span><span style="font-family:Verdana;">cise and low-cost method for quantification of SDZ and PYR using a </span><span style="font-family:Verdana;">non-so</span><span style="font-family:Verdana;">phisticate chromatographic equipment, suitable for hospital therapeutic </span><span style="font-family:Verdana;">monitoring for public health system.展开更多
Seed number(SN)is the component most strongly associated with yield in soybean.SN depends on pod number(PN)and seeds per pod(SPP).Whereas SPP is a relatively stable component,PN is strongly influenced by environmental...Seed number(SN)is the component most strongly associated with yield in soybean.SN depends on pod number(PN)and seeds per pod(SPP).Whereas SPP is a relatively stable component,PN is strongly influenced by environmental and management factors.However,the environmental cues involved in PN regulation are not completely understood.The influence of increasing SPP on other yield components is controversial.Field trials were conducted in two growing seasons using two pairs of lanceolate(L)and ovate(O)nearisogenic lines,sown at low(LD)and high(HD)plant densities to evaluate the effect of leaflet shape on crop growth parameters,canopy red/far-red(R/FR)ratio,their relationships with pod initiation,PN,and yield;and the effect of increasing SPP on PN,SN,and yield.L canopies showed a higher number of pods initiated(PI)than O canopies owing to the increase of PI on branches.No association between PI and crop growth rate during the pod set period was found.PI was negatively associated with leaf area index(LAI)and light interception.In contrast,a positive association between PI and canopy R/FR ratio was found.This latter association was sustained irrespective of whether the LAI was below or above its critical value,providing experimental evidence that R/FR ratio is positively associated with pod initiation in soybean canopies.An increase in SPP produced a direct and steady increase in SN regardless of PN and plant density.A yield increase was observed for the L-LD treatment,which combined the increased SPP of L lines with the highest PN of L-LD canopies.These results have implications for crop management and breeding strategies aimed at increasing the yield potential of soybean.展开更多
Colorful thin oxide films were synthesized by galvanostatic anodization on Ti−6Al−4V alloy.Three different aqueous solutions containing corrosion inorganic inhibitors(Na_(2)MoO_(4),NaH_(2)PO_(4) and NH4VO3)were employ...Colorful thin oxide films were synthesized by galvanostatic anodization on Ti−6Al−4V alloy.Three different aqueous solutions containing corrosion inorganic inhibitors(Na_(2)MoO_(4),NaH_(2)PO_(4) and NH4VO3)were employed for the anodization treatment.The effect of inhibitor anions on the corrosion behavior of the alloy in Ringer solution was studied.Open circuit potential(OCP),Tafel polarization,linear sweep voltammetry(LSV)and chronoamperometry(CA)were performed to evaluate the corrosion performance of the treated electrodes.The incorporation of the inhibitor ions was detected by the release of Mo,V and P through ICP-AES technique.The formed oxides were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS).The results show that compact,amorphous oxides without pores or cracks were obtained independently of the solution used.The sample anodized in Na_(2)MoO_(4) solution registered the lowest corrosion current density(0.11μA/cm^(2)),and it was able to protect the alloy even after 168 h of immersion in Ringer solution.No cracks or corrosion products were detected.The XPS analysis reveals the incorporation of molybdenum to the oxide film in the form of Mo^(6+) and Mo^(4+).展开更多
The fossil record of terrestrial mammals in Antarctica is temporally and geographically constrained to the Eocene outcrops of La Meseta and Submeseta formations in Seymour(Marambio)Island in West Antarctica.The faunal...The fossil record of terrestrial mammals in Antarctica is temporally and geographically constrained to the Eocene outcrops of La Meseta and Submeseta formations in Seymour(Marambio)Island in West Antarctica.The faunal assemblage indicates a clear South American imprint since all the groups have a close phylogenetic relationship with Cretaceous and Paleogene mammals from Patagonia.Despite the presence of several mammalian taxonomic groups:Dryolestida,Gondwanatheria,Eutheria and Metatheria,the presence of other major mammalian taxa should be expected and will probably be confirmed by new findings.Placental mammals with an inferred body mass between 10 to 400 kg in size,are represented by xenarthrans,and two groups of the so called South American native ungulates:Astrapotheria and Litopterna.The Metatheria are the smaller(less than 1 kg)and most abundant components of the fauna.Marsupials are represented by derorhynchid ameridelphians,several microbiotherian australidelphians(both microbiotheriids and woodburnodontids),and?glasbiid prepidolopod and polydolopid polydolopimorphians.Plus,there are remains of several mammalian teeth of indeterminate phylogenetic affinities.The present knowledge of the Southern Hemisphere mammalian evolution and paleogeographic change through time,indicates that Antarctica played a major role for land mammals,at least since the Jurassic.The actual representation of Paleogene terrestrial mammals in Antarctica is most probably biased,as all the evidence indicates that australosphenidan mammals should be present in this continent since the Jurassic.展开更多
Colorectal cancer(CRC)remains the third most prevalent cancer disease and involves a multi-step process in which intestinal cells acquire malignant characteristics.It is well established that the appearance of distal ...Colorectal cancer(CRC)remains the third most prevalent cancer disease and involves a multi-step process in which intestinal cells acquire malignant characteristics.It is well established that the appearance of distal metastasis in CRC patients is the cause of a poor prognosis and treatment failure.Nevertheless,in the last decades,CRC aggressiveness and progression have been attributed to a specific cell population called CRC stem cells(CCSC)with features like tumor initiation capacity,self-renewal capacity,and acquired multidrug resistance.Emerging data highlight the concept of this cell subtype as a plastic entity that has a dynamic status and can be originated from different types of cells through genetic and epigenetic changes.These alterations are modulated by complex and dynamic crosstalk with environmental factors by paracrine signaling.It is known that in the tumor niche,different cell types,structures,and biomolecules coexist and interact with cancer cells favoring cancer growth and development.Together,these components constitute the tumor microenvironment(TME).Most recently,researchers have also deepened the influence of the complex variety of microorganisms that inhabit the intestinal mucosa,collectively known as gut microbiota,on CRC.Both TME and microorganisms participate in inflammatory processes that can drive the initiation and evolution of CRC.Since in the last decade,crucial advances have been made concerning to the synergistic interaction among the TME and gut microorganisms that condition the identity of CCSC,the data exposed in this review could provide valuable insights into the biology of CRC and the development of new targeted therapies.展开更多
Antarctica has significant environmental,scientific,historic,and intrinsic values,all of which are worth protecting into the future.This continent has a discrete number of places of scientific interest that exhibit gr...Antarctica has significant environmental,scientific,historic,and intrinsic values,all of which are worth protecting into the future.This continent has a discrete number of places of scientific interest that exhibit great potential as natural heritage sites;its geodiversity is of fundamental importance to scientific values of the continent,and the pursuit of geological and paleontological knowledge has had a strong influence on its historical values.Seymour Island was once called the‘Rosetta Stone’of Southern Hemisphere paleobiology,because this small island provides the most complete and richly fossiliferous Late Cretaceous–Paleogene sequence in Antarctica.In particular,fossil vertebrates form part of the evidence used in reconstructing the history of life on Antarctica.Paleontological heritage is considered a subset of geo-heritage that embodies both natural and historical components which has received only indirect recognition.Seymour Island is an outstanding paleontological area with high heritage value of its Late Cretaceous/Paleogene vertebrates and should be considered for geo-conservation and protection.This paper reviews vertebrate fossil occurrences and outcrops on Seymour Island and discusses some threats to these fossil sites.展开更多
Until recently,microglia were mainly known as the resident phagocytes of the brain,i.e.the‘immunological warriors’of the brain.However,extensive knowledge is being accumulated about the functions of microglia beyond...Until recently,microglia were mainly known as the resident phagocytes of the brain,i.e.the‘immunological warriors’of the brain.However,extensive knowledge is being accumulated about the functions of microglia beyond immunity.Nowadays,it is well accepted that microglial cells are highly dynamic and responsive,and that they intervene in a dual manner in many developmental processes that shape the central nervous system,including neurogenesis,gliogenesis,spatial patterning,synaptic formation and elimination,and neural circuit establishment and maturation.The differentiation and the pool of precursor cells were also shown to be under microglia regulation via bidirectional communication.In this concise review,I discuss our recent work in microglia-Pax6+cell interactions in one of the circumventricular organs,the pineal gland.An analogy with the rest of the central nervous system is also presented.In addition,I briefly examine mechanisms of interaction between microglia and non-microglial cells in both health and disease.New avenues are also introduced,which may lead us to better comprehend the impact of microglia in physiological and pathological conditions.展开更多
Under the present changing climate conditions and the observed temperature increase,it is of high importance to understand its effects on aquatic microbial life,and organisms’adaptations at the biochemical level.To a...Under the present changing climate conditions and the observed temperature increase,it is of high importance to understand its effects on aquatic microbial life,and organisms’adaptations at the biochemical level.To adjust to temperature or salinity stress and avoid cell damage,organisms alter their degree of fatty acids(FAs)saturation.Thus,temperature is expected to have strong effects on both the quantity and quality of FAs in aquatic microorganisms.Here we review some recent findings about FAs sensitivity to climate change in contrasting environments.Overall,heat waves may induce changes in the relative abundance of polyunsaturated FAs(PUFA).However,the impact of the exposure to warming waters is different in temperate and polar environments.In cold marine waters,high concentration of omega-3(ω3)FAs such as eicosapentaenoic acid(EPA)is promoted due to the activation of the desaturase enzyme.In this way,cells have enough energy to produce or activate antioxidant protection mechanisms and avoid oxidative stress due to heat waves.Contrastingly,under high irradiance and heat wave conditions in temperate environments,photosystems’protection is achieved by decreasing EPA concentration due to desaturase sensitivity.Essential FAs are transferred in aquatic food webs.Therefore,any alteration in the production of essential FAs by phytoplankton(the main source ofω3)due to climate warming can be transferred to higher trophic levels,with cascading effects for the entire aquatic ecosystem.展开更多
The World Health Organization has predicted that neurodegenerative diseases affecting the motor function will become the second most prevalent cause of death in the next 20 years.New therapies can result from three ma...The World Health Organization has predicted that neurodegenerative diseases affecting the motor function will become the second most prevalent cause of death in the next 20 years.New therapies can result from three main sources:synthetic compounds,natural products,and existing drugs.Parkinson’s disease(PD)is a common neurodegenerative disease affecting 1–3%of the adult population over 50 years of age worldwide.It is initially characterized by the death of dopaminergic neurons in the substantia nigra pars compact and later by the widespread loss of nondopaminergic neurons,including those in the cortex.展开更多
The objective was to reduce saturated fatty acids (SFA) and increase conjugated linoleic acid (CLA, cis-9, trans-11 C18:2), α-linolenic (cis-9, cis-12, cis-15 C18:3) and docosahexaenoic (DHA, C22:6) contents in milk ...The objective was to reduce saturated fatty acids (SFA) and increase conjugated linoleic acid (CLA, cis-9, trans-11 C18:2), α-linolenic (cis-9, cis-12, cis-15 C18:3) and docosahexaenoic (DHA, C22:6) contents in milk from confined dairy cows in order to promote a healthier option. The work was carried out in a commercial farm (Talar) located in Laguna del Sauce, Maldonado (Uruguay). Twenty four cows were assigned to one of two treatments (12 cows per treatment) over a 6 weeks experimental period. Treatments consisted in a control total mixed ration (C-TMR) without supplementary lipids (L) or the same TMR with the addition of 0.144 kg/cow·day of algae and 0.72 kg/cow·day of soybean oil (L-TMR). Chemical composition of the TMR (44.27% DM) averaged 15.94% for crude protein (CP), 38.20% neutral detergent fiber (NDF), 20.36% acid detergent fiber (ADF), 5.56% fat, 5.30% ash and 28.6% nonstructural carbohydrate (NSCH) with 1.81 Mcal/kg of net energy for lactation (NEL). After 39 days of feeding, individual milk samples were collected during three consecutive days. From the total milk collected, 20 ml were immediately used for chemical composition (Milko Scan) and 80 ml for analysis for milk FA profile. From week 3 onwards, milk production (kg/cow·day) resulted higher (P P P = 0.43) on milk protein content (C-TMR = 3.20;L-TMR = 3.07 g/100g). Milk lactose (C-TMR = 4.86, L-TMR = 4.69 g/100g) and urea nitrogen contents (C-TMR = 21.18, L-TMR = 17.33 g/100g) tended (P cow·day, P 12:0 to C16:0 FA averaging 45.19 in C-TMR and 34.74 g/100g in L-TMR (P P 18:1 trans-9) (0.23) and C18:1 trans-10 (0.44) FA increased (P trans-11 C18:1, VA) increased from 1.08 in C-TMR to 2.56 g/100g of FA in L-TMR (P cis-9, trans-11 C18:2) increased (127%) from 0.62 in C-TMR to 1.41 g/100g FA in L-TMR milk. Content of α-linolenic acid resulted 20% higher (P P cis-9 C18:1) resulted higher (P α-linolenic).展开更多
Phosphosites in the human proteome represent an excellent source of potential biomarkers of pesticide toxicity. In fact, experimental animal models as well as in vitro studies have revealed phosphorylation disruption ...Phosphosites in the human proteome represent an excellent source of potential biomarkers of pesticide toxicity. In fact, experimental animal models as well as in vitro studies have revealed phosphorylation disruption associated to metabolic regulation, hormone signaling, neuronal function and differentiation, cell survival and death. Due to their estrogen-mimicking ability, pesticides are considered as prime etiological suspects of increasing tumor incidence. Evidences of alterations in the signal transduction pathways involved in the tumor progression stage of pesticides were also provided. Despite progress in understanding the effect of pesticides on the human phosphorproteome and their health outcomes, it remains a complex issue to be studied. By now, the potential impact of pesticides in epigenetic phosphorylation pathways remains poorly explored. In addition, studies involving pesticides mixtures effects are needed. This review updates and provides a comprehensive discussion on the molecular and biochemical events underlying protein phosphorylation pathway disruption caused by pesticides most frequently detected in human tissues and fluids, such as organochlorine pesticides and organophosphates. The link between epidemiological studies and experimental approaches is also considered. Future challenges, such as micro-array phosphoproteome studies to complement gene expression arrays to understand the mechanisms involved in pesticide toxicology are briefly discussed.展开更多
The San Rafael Block(SRB)is part of one of the main retroarc volcanic provinces in southern Central Andes in Mendoza,Argentina.This block is located in the Andean foothills between the orogenic front and foreland base...The San Rafael Block(SRB)is part of one of the main retroarc volcanic provinces in southern Central Andes in Mendoza,Argentina.This block is located in the Andean foothills between the orogenic front and foreland basement uplifts of late Miocene age.In order to analyze the geochronological evolution of the Quaternary volcanism in the region,several geologic and geophysical studies have been conducted.Nevertheless,the crust,where the SRB is located,has not been well characterized yet.Based on gravimetric and magnetic data,together with isostatic and elastic thickness analyses,we modeled the crustal structure of the area.Information obtained has allowed us to understand the crust where the SRB and the Payenia volcanic province are located.Bouguer anomalies indicate that the SRB presents higher densities to the North of Cerro Nevado and Moho calculations suggest depths for this block between 40 and 50 km.Determinations of elastic thickness would indicate that the crust supporting the San Rafael Block presents values of approximately 10 km,being enough to support the block loading.However,in the Payenia region,elastic thickness values are close to zero due to the regional temperature increase.展开更多
基金supported by the Consejo Nacional de Investigaciones Cientıfícas y Técnicas(CONICET)(PIP 2021-2023/11220200102779CO).
文摘Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patient with American tegumentary leishmaniasis in Mendoza,Argentina,a non-endemic region.Patient concerns:A 43-year-old male was admitted to a tertiary care hospital in Mendoza,Argentina Republic with a history of progressive nasal discharge,septal perforation,facial pain,and pruritus.Despite treatment for presumed nasal myiasis and vasculitis with granulomatosis,symptoms persisted.Diagnosis:American tegumentary leishmaniasis.Interventions:Intravenous liposomal amphotericin B.Outcomes:Follow-up at 30 days showed no recurrence of symptoms with a remarkable clinical improvement of the nasal lesion.Lessons:This case sheds light on the necessity of accurate identification for timely intervention and the need to recognize the diverse manifestations of American tegumentary leishmaniasis to avoid misdiagnosis.
基金funded by the Agencia Nacional de Promoción Científica y Tecnológica-Proyectos de Investigación Científica y Tecnológica-PICT 2017-0607(to M.Reguero)and PICT 2019-00327(to M.Buono).
文摘The Antarctic Paleogene marine fossil record has been the key to reconstructing the evolution of the Weddellian Sea and final dismemberment of Southern Gondwana.In this context,Eocene marine vertebrates from Seymour(Marambio)Island have provided valuable information.We present the first Eocene record of marine reptiles from the southern Atlantic Coast of South America.This corresponds to several postcranial turtle remains represented by a proximal end of the right humerus,three caudal and one thoracic vertebrae,a fragment of the left pubis,and ten ossicles of the dorsal carapace,coming from the Leticia Formation(late-mid Eocene)at Cabo Tiburones,Tierra del Fuego,Argentina.These materials show severalfeatures such as the size and general morphology of the humerus and vertebrae,and the presence of relatively small,irregular,smooth,and unkeeled ossicles,which allow us to assign them to Dermochelyidae indet.Dermochelyids are a cosmopolitan group ofcryptodiran turtles,registered from the late Cretaceous up to the recent,with some physiological-biological peculiarities(e.g.,endothermy and an exclusive jellyfish-based diet)and characterized by the presence of an osseous carapace formed by ossicles.The new finding from the Leticia Formation is an addition to the scarce and extremely fragmentary record of Eocene dermochelyids from the southern seas like those from the La Meseta and Submeseta formations(Antarctica)and the Waihao and Burnside formations(New Zealand).This new information allows us to discuss the presence of these turtles in such high latitudes in the past and its implication in the evolution of the Weddellian fauna.
基金Nacional de Promoción Científica y Tecnológica(Grant:PICTO 2010-0111)the Instituto Antártico Argentino-Dirección Nacional del Antártico(PINST-05)provided financial and logistical support.
文摘In this work,we report long-term trends in the abundance and breeding performance of Adélie penguins(Pygoscelis adeliae)nesting in three Antarctic colonies(i.e.,at Martin Point,South Orkneys Islands;Stranger Point/Cabo Funes,South Shetland Islands;and Esperanza/Hope Bay in the Antarctic Peninsula)from 1995/96 to 2022/23.Using yearly count data of breeding groups selected,we observed a decline in the number of breeding pairs and chicks in crèche at all colonies studied.However,the magnitude of change was higher at Stranger Point than that in the remaining colonies.Moreover,the index of breeding success,which was calculated as the ratio of chicks in crèche to breeding pairs,exhibited no apparent trend throughout the study period.However,it displayed greater variability at Martin Point compared to the other two colonies under investigation.Although the number of chicks in crèche of Adélie penguins showed a declining pattern,the average breeding performance was similar to that reported in gentoo penguin colonies,specifically,those undergoing a population increase(even in sympatric colonies facing similar local conditions).Consequently,it is plausible to assume a reduction of the over-winter survival as a likely cause of the declining trend observed,at least in the Stranger Point and Esperanza colonies.However,we cannot rule out local effects during the breeding season affecting the Adélie population of Martin Point.
基金To the Universidad Nacional de La Plata PID N955,ANPCyT PICT 20170607,and CONICET PIP 0096 for partial support.
文摘The worldwide record of Cretaceous Neornithes and Neornithine-like birds is both controversial and poorly understood.However,in recent years,the Antarctic continent has yielded a substantial number of Maastrichtian avian specimens,contributing significantly to our understanding of the early evolution of this group.Simultaneously,the keen interest and collaborative efforts of various paleornithologists have resulted in a wealth of knowledge,which we have thoroughly reviewed,updated,and discussed in the context of recent discoveries.
基金Supported by Agencia Nacional de Promoción Científica y Tecnológica(PICTO-2021-COVID secuelas-00005 to JQ).
文摘In coronavirus disease 2019(COVID-19),severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)primarily targets the respiratory system,but evidence suggests extrapulmonary organ involvement,notably in the liver.Viral RNA has been detected in hepatic tissues,and in situ hybridization revealed virions in blood vessels and endothelial cells.Electron microscopy confirmed viral particles in hepatocytes,emphasizing the need for understanding hepatotropism and direct cytopathic effects in COVID-19-related liver injury.Various factors contribute to liver injury,including direct cytotoxicity,vascular changes,inflammatory responses,immune reactions from COVID-19 and vaccinations,and druginduced liver injury.Although a typical hepatitis presentation is not widely documented,elevated liver biochemical markers are common in hospitalized COVID-19 patients,primarily showing a hepatocellular pattern of elevation.Long-term studies suggest progressive cholestasis may affect 20%of patients with chronic liver disease post-SARS-CoV-2 infection.The molecular mechanisms underlying SARS-CoV-2 infection in the liver and the resulting liver damage are complex.This“Editorial”highlights the expression of the Angiotensin-converting enzyme-2 receptor in liver cells,the role of inflammatory responses,the impact of hypoxia,the involvement of the liver's vascular system,the infection of bile duct epithelial cells,the activation of hepatic stellate cells,and the contribution of monocyte-derived macrophages.It also mentions that pre-existing liver conditions can worsen the outcomes of COVID-19.Understanding the interaction of SARS-CoV-2 with the liver is still evolving,and further research is required.
基金Thanks to the Agencia Nacional de Promoción Científica y Tecnológica for financial support trough PICT 2017-0607(to MR),PICT 2019-02419(to SGC).
文摘In recent years,the taxonomy and systematics of the cartilaginous fish taxa from the Eocene La Meseta Formation of Seymour(Marambio)Island,Antarctica have been extensively discussed in a series of papers,resulting in a complete revision of the Antarctic Eocene ichthyofauna housed in the Vertebrate Paleontology collection of the Museo de La Plata,Argentina.This collection constitutes one of the largest and taxonomically most diverse in the world,with approximately 20000 specimens,which provides a solid database used for the analysis of qualitative and quantitative chondrichthyan taxonomic composition.The information provided herein might be useful to understand the sudden decline in chondrichthyan taxonomic diversity towards the top of La Meseta Formation as well as its potential relationship with environmental changes during the Eocene.
基金supported by Núcleo Milenio EVOTEM from ANIDGobierno de Chile
文摘The first dinosaur discovered in the Antarctic continent was the ankylosaur Antarctopelta oliveroi in the 1980s.Nevertheless,since then several hypotheses of phylogenetical relationships have been proposed because these have been depended on how the skeletal remains have been interpreted.The main obstacle for clarifying its phylogenetic position is that many portions of the skeleton remain unknown,in addition to the presence of unknown characters in typical ankylosaurs.Considered an ankylosaurid,nodosaurid,or even a chimaera,a recent proposal based on mostly complete material of a new ankylosaur from Chilean Patagonia provided support for a novel phylogenetic hypothesis:Antarctopelta and other southern ankylosaurs are an early branching clade,the Parankylosauria,whose origin probably dates to the Late Jurassic.In the light of this new view,a redescription of the available skeletal remains is provided together with a new reconstruction of the first Antarctic dinosaur known to the science community.
基金This work was supported with Funds from Agencia Nacional de Promoción Científica y Tecnoló gica (PICT 2012-0892,BID, PICT 2014-1388, BID)CONICET (PIP 112-201201-00259 CO)+2 种基金CONICET-UNJU (PIO 1402014100133)UNJU (SECTER A 0176 and B 046)CEBio NGO, Idea Wild, Association of Field Ornithologists, Optic for the Tropic, and Rufford Small Grants
文摘In tropical and subtropical forest ecosystems,cavities formed by decay processes are a key but scarce resource for birds that nest and roost in them,which makes them a highly sensitive group to logging.The piedmont forest of northwestern Argentina is a complex ecosystem with 113 tree and 120 bird species.It has high logging pressure on the few,well-conserved forest remnants,complicating the delineation of sustainable management guidelines for each tree or bird species in a short time.Our objective was to reduce the complexity of subtropical forests by grouping tree species according to the characteristics used by secondary cavity-nesting birds(i.e.non excavators).In the piedmont forest,50 plots of 0.25 ha were sampled to record cavity trees and cavity characteristics.These were then used in a cluster analysis to form tree groups.Additionally,cavities were searched to identify the bird species using the decay-formed cavities.A total of 187 cavity trees,comprising 23 tree species,were recorded,and these formed four tree groups or clusters.We recorded 86 cavities that were used by secondary cavitynesting bird species.The four tree groups were unequally used by secondary cavity nesters.The tree group that included valuable timber species(Myroxylon peruiferum,Anadenanthera colubrina and Calycophyllum multiflorum)and had the greatest cavity availability represented 71%of total cavity use.Another tree group with valuable timber species(Cedrela balansae and Amburana cearensis),measured>73 cm DBH and>21 m tall,had cavity entrances>0.10 cm2,and contributed 14%of all cavity use by birds.A third group had no highly economically valuable tree species,and included the snag category(i.e.standing dead trees)as well as a 15%of cavity use.The fourth tree group had a DBH<0.40 cm,only one highly economically valuable tree species(Cordia trichotoma),and supported no cavity use.The clustering of subtropical trees can reduce the complexity of these forests,hence easing their management by focusing on those groups with tree species showing similar characteristics and providing suitable nesting sites for secondary cavity-nesting birds.
文摘<span style="font-family:""><span style="font-family:Verdana;">Infection with </span><i><span style="font-family:Verdana;">Toxoplasma gondii</span></i><span style="font-family:Verdana;">, is one of the most widespread zoonoses in the world. Congenital Toxoplasmosis (CT) is particularly risky due to its fetal </span><span style="font-family:Verdana;">complications. Sulfadiazine (SDZ) and Pyrimethamine (PYR) are usually </span><span style="font-family:Verdana;">used </span><span style="font-family:Verdana;">for CT treatment in Argentina, to prevent morbidity. Due to the lack of </span><span style="font-family:Verdana;">commercial pediatric formulations, these must be prepared in the hospital pharmacy. This is the first report of serum concentrations measures in pediatric CT therapy for this combination of drugs. A bioanalytical method was developed for identification and simultaneous quantification of SDZ and PYR by High Performance Liquid Chromatography (HPLC) with UV detection. The validated method was applied to residual serum samples obtained from 6 pediatric patients undergoing treatment with SDZ 42.20 a 93.70 mg/kg/day and </span><span style="font-family:Verdana;">PYR 0.77 a 2.70 mg/kg/day. Sample pretreatment consisted </span></span><span style="font-family:Verdana;">on</span><span style="font-family:Verdana;"> a</span><span style="font-family:""><span style="font-family:Verdana;"> deproteini</span><span style="font-family:Verdana;">zation step followed by centrifugation and then injection of supernatant.</span><span style="font-family:Verdana;"> Limit of Detection (LOD) and Quantification (LOQ) were (0.17 ± 0.02 and 0.13 ± 0.02) μg/mL and (0.46 ± 0.01 and 0.36 ± 0.01) μg/mL for SDZ and PYR respectively, with an appropriate linear range. Concentrations range found </span><span style="font-family:Verdana;">were (<LOD</span></span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">162.04 ± 0.02) μg/mL for SDZ and (<LOD</span><span style="font-family:Verdana;"> - </span><span style="font-family:Verdana;">7.30 ± 0.03) </span><span style="font-family:""><span style="font-family:Verdana;">μg/mL for PYR. We developed and validated in real pediatric samples, an acute, pre</span><span style="font-family:Verdana;">cise and low-cost method for quantification of SDZ and PYR using a </span><span style="font-family:Verdana;">non-so</span><span style="font-family:Verdana;">phisticate chromatographic equipment, suitable for hospital therapeutic </span><span style="font-family:Verdana;">monitoring for public health system.
基金supported by Project PUE 22920160100043,IICAR-CONICET,ArgentinaProject 2010-205-13 Ministerio de Ciencia Tecnología e Innovación Productiva,Santa Fe Province,Argentina.
文摘Seed number(SN)is the component most strongly associated with yield in soybean.SN depends on pod number(PN)and seeds per pod(SPP).Whereas SPP is a relatively stable component,PN is strongly influenced by environmental and management factors.However,the environmental cues involved in PN regulation are not completely understood.The influence of increasing SPP on other yield components is controversial.Field trials were conducted in two growing seasons using two pairs of lanceolate(L)and ovate(O)nearisogenic lines,sown at low(LD)and high(HD)plant densities to evaluate the effect of leaflet shape on crop growth parameters,canopy red/far-red(R/FR)ratio,their relationships with pod initiation,PN,and yield;and the effect of increasing SPP on PN,SN,and yield.L canopies showed a higher number of pods initiated(PI)than O canopies owing to the increase of PI on branches.No association between PI and crop growth rate during the pod set period was found.PI was negatively associated with leaf area index(LAI)and light interception.In contrast,a positive association between PI and canopy R/FR ratio was found.This latter association was sustained irrespective of whether the LAI was below or above its critical value,providing experimental evidence that R/FR ratio is positively associated with pod initiation in soybean canopies.An increase in SPP produced a direct and steady increase in SN regardless of PN and plant density.A yield increase was observed for the L-LD treatment,which combined the increased SPP of L lines with the highest PN of L-LD canopies.These results have implications for crop management and breeding strategies aimed at increasing the yield potential of soybean.
基金The financial supports of the Secretaría de Ciencia y Técnica-UNS (PGI 24/M159)the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-PIP 112-20150-100147)the Agencia Nacional de Promoción Científica y Tecnológica (ANPCy T-PICT 2015-0726)。
文摘Colorful thin oxide films were synthesized by galvanostatic anodization on Ti−6Al−4V alloy.Three different aqueous solutions containing corrosion inorganic inhibitors(Na_(2)MoO_(4),NaH_(2)PO_(4) and NH4VO3)were employed for the anodization treatment.The effect of inhibitor anions on the corrosion behavior of the alloy in Ringer solution was studied.Open circuit potential(OCP),Tafel polarization,linear sweep voltammetry(LSV)and chronoamperometry(CA)were performed to evaluate the corrosion performance of the treated electrodes.The incorporation of the inhibitor ions was detected by the release of Mo,V and P through ICP-AES technique.The formed oxides were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS).The results show that compact,amorphous oxides without pores or cracks were obtained independently of the solution used.The sample anodized in Na_(2)MoO_(4) solution registered the lowest corrosion current density(0.11μA/cm^(2)),and it was able to protect the alloy even after 168 h of immersion in Ringer solution.No cracks or corrosion products were detected.The XPS analysis reveals the incorporation of molybdenum to the oxide film in the form of Mo^(6+) and Mo^(4+).
基金supported by Instituto Antártico Argentino–Dirección Nacional del Antártico (IAA–DNA)the funding PIP 0489 and N812-UNLP Vertebrados del lapso Cretácico Superior Paleógeno de la Península Antártica y extremo sur de Patagonia (Sur del Paralelo 50°)
文摘The fossil record of terrestrial mammals in Antarctica is temporally and geographically constrained to the Eocene outcrops of La Meseta and Submeseta formations in Seymour(Marambio)Island in West Antarctica.The faunal assemblage indicates a clear South American imprint since all the groups have a close phylogenetic relationship with Cretaceous and Paleogene mammals from Patagonia.Despite the presence of several mammalian taxonomic groups:Dryolestida,Gondwanatheria,Eutheria and Metatheria,the presence of other major mammalian taxa should be expected and will probably be confirmed by new findings.Placental mammals with an inferred body mass between 10 to 400 kg in size,are represented by xenarthrans,and two groups of the so called South American native ungulates:Astrapotheria and Litopterna.The Metatheria are the smaller(less than 1 kg)and most abundant components of the fauna.Marsupials are represented by derorhynchid ameridelphians,several microbiotherian australidelphians(both microbiotheriids and woodburnodontids),and?glasbiid prepidolopod and polydolopid polydolopimorphians.Plus,there are remains of several mammalian teeth of indeterminate phylogenetic affinities.The present knowledge of the Southern Hemisphere mammalian evolution and paleogeographic change through time,indicates that Antarctica played a major role for land mammals,at least since the Jurassic.The actual representation of Paleogene terrestrial mammals in Antarctica is most probably biased,as all the evidence indicates that australosphenidan mammals should be present in this continent since the Jurassic.
基金Supported by Agencia Nacional de Promoción Científica y Tecnológica,No.PICT-2020-SERIEA-03440 and PICT-2013-1441Consejo Nacional de Investigaciones Científicas y Técnicas,No.PIP11220200103061CO and PIP11220150100350CO+3 种基金Instituto Nacional del Cáncer Asistencia Financiera Ⅱ,RESOL 493/14,No.2002-4395-14-1Instituto Nacional del Cáncer Asistencia Financiera Ⅲ-2016-2017,RESOL-2016-1006-E-APN-MS,No.2002-3862-16-1 CANCERUniversidad Nacional del Sur,No.PGI:24/B230 and PGI:24/B303Fundación Alberto J Roemmers of Argentina.
文摘Colorectal cancer(CRC)remains the third most prevalent cancer disease and involves a multi-step process in which intestinal cells acquire malignant characteristics.It is well established that the appearance of distal metastasis in CRC patients is the cause of a poor prognosis and treatment failure.Nevertheless,in the last decades,CRC aggressiveness and progression have been attributed to a specific cell population called CRC stem cells(CCSC)with features like tumor initiation capacity,self-renewal capacity,and acquired multidrug resistance.Emerging data highlight the concept of this cell subtype as a plastic entity that has a dynamic status and can be originated from different types of cells through genetic and epigenetic changes.These alterations are modulated by complex and dynamic crosstalk with environmental factors by paracrine signaling.It is known that in the tumor niche,different cell types,structures,and biomolecules coexist and interact with cancer cells favoring cancer growth and development.Together,these components constitute the tumor microenvironment(TME).Most recently,researchers have also deepened the influence of the complex variety of microorganisms that inhabit the intestinal mucosa,collectively known as gut microbiota,on CRC.Both TME and microorganisms participate in inflammatory processes that can drive the initiation and evolution of CRC.Since in the last decade,crucial advances have been made concerning to the synergistic interaction among the TME and gut microorganisms that condition the identity of CCSC,the data exposed in this review could provide valuable insights into the biology of CRC and the development of new targeted therapies.
基金Funding for this study was provided by PICT 0607-2018 and UNLP 11N812
文摘Antarctica has significant environmental,scientific,historic,and intrinsic values,all of which are worth protecting into the future.This continent has a discrete number of places of scientific interest that exhibit great potential as natural heritage sites;its geodiversity is of fundamental importance to scientific values of the continent,and the pursuit of geological and paleontological knowledge has had a strong influence on its historical values.Seymour Island was once called the‘Rosetta Stone’of Southern Hemisphere paleobiology,because this small island provides the most complete and richly fossiliferous Late Cretaceous–Paleogene sequence in Antarctica.In particular,fossil vertebrates form part of the evidence used in reconstructing the history of life on Antarctica.Paleontological heritage is considered a subset of geo-heritage that embodies both natural and historical components which has received only indirect recognition.Seymour Island is an outstanding paleontological area with high heritage value of its Late Cretaceous/Paleogene vertebrates and should be considered for geo-conservation and protection.This paper reviews vertebrate fossil occurrences and outcrops on Seymour Island and discusses some threats to these fossil sites.
基金Supported by grants from CONICET(Argentina,EM,PIP-CONICET 112-201101-00247,http://www.conicet.gov.ar)ANPCyT(Argentina,EM,PICT 2012-174,PICT 2017-499+1 种基金http://www.agencia.mincyt.gob.ar)NIH-CONICET(Argentina and USA,EM and Stephen Noctor,F65096).
文摘Until recently,microglia were mainly known as the resident phagocytes of the brain,i.e.the‘immunological warriors’of the brain.However,extensive knowledge is being accumulated about the functions of microglia beyond immunity.Nowadays,it is well accepted that microglial cells are highly dynamic and responsive,and that they intervene in a dual manner in many developmental processes that shape the central nervous system,including neurogenesis,gliogenesis,spatial patterning,synaptic formation and elimination,and neural circuit establishment and maturation.The differentiation and the pool of precursor cells were also shown to be under microglia regulation via bidirectional communication.In this concise review,I discuss our recent work in microglia-Pax6+cell interactions in one of the circumventricular organs,the pineal gland.An analogy with the rest of the central nervous system is also presented.In addition,I briefly examine mechanisms of interaction between microglia and non-microglial cells in both health and disease.New avenues are also introduced,which may lead us to better comprehend the impact of microglia in physiological and pathological conditions.
基金The research leading to these results further received funding grants ANPCYT PICT 2011-130 Raíces of the Agencia Nacional de Promociones Científicas of Argentina to IRS and MPH,and from the European Union’s Horizon 2020 research and innovation program under grant agreement No.730984,ASSEMBLEPLUS2019359 Project awarded to FDIt is further a contribution to CoastCarb(Funding ID 872609,H2020,MSCA-RISE-2019,Research and Innovation Staff Exchange).
文摘Under the present changing climate conditions and the observed temperature increase,it is of high importance to understand its effects on aquatic microbial life,and organisms’adaptations at the biochemical level.To adjust to temperature or salinity stress and avoid cell damage,organisms alter their degree of fatty acids(FAs)saturation.Thus,temperature is expected to have strong effects on both the quantity and quality of FAs in aquatic microorganisms.Here we review some recent findings about FAs sensitivity to climate change in contrasting environments.Overall,heat waves may induce changes in the relative abundance of polyunsaturated FAs(PUFA).However,the impact of the exposure to warming waters is different in temperate and polar environments.In cold marine waters,high concentration of omega-3(ω3)FAs such as eicosapentaenoic acid(EPA)is promoted due to the activation of the desaturase enzyme.In this way,cells have enough energy to produce or activate antioxidant protection mechanisms and avoid oxidative stress due to heat waves.Contrastingly,under high irradiance and heat wave conditions in temperate environments,photosystems’protection is achieved by decreasing EPA concentration due to desaturase sensitivity.Essential FAs are transferred in aquatic food webs.Therefore,any alteration in the production of essential FAs by phytoplankton(the main source ofω3)due to climate warming can be transferred to higher trophic levels,with cascading effects for the entire aquatic ecosystem.
基金supported by Grants PIP 0906CO from CONICETPICT 3776 from Agencia Nacional de Promoción Científica y Tecnológica(ANPCyT)CIUNSa 2530/0 from Universidad Nacional de Salta and PIUNT D641/1(all to LA)
文摘The World Health Organization has predicted that neurodegenerative diseases affecting the motor function will become the second most prevalent cause of death in the next 20 years.New therapies can result from three main sources:synthetic compounds,natural products,and existing drugs.Parkinson’s disease(PD)is a common neurodegenerative disease affecting 1–3%of the adult population over 50 years of age worldwide.It is initially characterized by the death of dopaminergic neurons in the substantia nigra pars compact and later by the widespread loss of nondopaminergic neurons,including those in the cortex.
文摘The objective was to reduce saturated fatty acids (SFA) and increase conjugated linoleic acid (CLA, cis-9, trans-11 C18:2), α-linolenic (cis-9, cis-12, cis-15 C18:3) and docosahexaenoic (DHA, C22:6) contents in milk from confined dairy cows in order to promote a healthier option. The work was carried out in a commercial farm (Talar) located in Laguna del Sauce, Maldonado (Uruguay). Twenty four cows were assigned to one of two treatments (12 cows per treatment) over a 6 weeks experimental period. Treatments consisted in a control total mixed ration (C-TMR) without supplementary lipids (L) or the same TMR with the addition of 0.144 kg/cow·day of algae and 0.72 kg/cow·day of soybean oil (L-TMR). Chemical composition of the TMR (44.27% DM) averaged 15.94% for crude protein (CP), 38.20% neutral detergent fiber (NDF), 20.36% acid detergent fiber (ADF), 5.56% fat, 5.30% ash and 28.6% nonstructural carbohydrate (NSCH) with 1.81 Mcal/kg of net energy for lactation (NEL). After 39 days of feeding, individual milk samples were collected during three consecutive days. From the total milk collected, 20 ml were immediately used for chemical composition (Milko Scan) and 80 ml for analysis for milk FA profile. From week 3 onwards, milk production (kg/cow·day) resulted higher (P P P = 0.43) on milk protein content (C-TMR = 3.20;L-TMR = 3.07 g/100g). Milk lactose (C-TMR = 4.86, L-TMR = 4.69 g/100g) and urea nitrogen contents (C-TMR = 21.18, L-TMR = 17.33 g/100g) tended (P cow·day, P 12:0 to C16:0 FA averaging 45.19 in C-TMR and 34.74 g/100g in L-TMR (P P 18:1 trans-9) (0.23) and C18:1 trans-10 (0.44) FA increased (P trans-11 C18:1, VA) increased from 1.08 in C-TMR to 2.56 g/100g of FA in L-TMR (P cis-9, trans-11 C18:2) increased (127%) from 0.62 in C-TMR to 1.41 g/100g FA in L-TMR milk. Content of α-linolenic acid resulted 20% higher (P P cis-9 C18:1) resulted higher (P α-linolenic).
文摘Phosphosites in the human proteome represent an excellent source of potential biomarkers of pesticide toxicity. In fact, experimental animal models as well as in vitro studies have revealed phosphorylation disruption associated to metabolic regulation, hormone signaling, neuronal function and differentiation, cell survival and death. Due to their estrogen-mimicking ability, pesticides are considered as prime etiological suspects of increasing tumor incidence. Evidences of alterations in the signal transduction pathways involved in the tumor progression stage of pesticides were also provided. Despite progress in understanding the effect of pesticides on the human phosphorproteome and their health outcomes, it remains a complex issue to be studied. By now, the potential impact of pesticides in epigenetic phosphorylation pathways remains poorly explored. In addition, studies involving pesticides mixtures effects are needed. This review updates and provides a comprehensive discussion on the molecular and biochemical events underlying protein phosphorylation pathway disruption caused by pesticides most frequently detected in human tissues and fluids, such as organochlorine pesticides and organophosphates. The link between epidemiological studies and experimental approaches is also considered. Future challenges, such as micro-array phosphoproteome studies to complement gene expression arrays to understand the mechanisms involved in pesticide toxicology are briefly discussed.
文摘The San Rafael Block(SRB)is part of one of the main retroarc volcanic provinces in southern Central Andes in Mendoza,Argentina.This block is located in the Andean foothills between the orogenic front and foreland basement uplifts of late Miocene age.In order to analyze the geochronological evolution of the Quaternary volcanism in the region,several geologic and geophysical studies have been conducted.Nevertheless,the crust,where the SRB is located,has not been well characterized yet.Based on gravimetric and magnetic data,together with isostatic and elastic thickness analyses,we modeled the crustal structure of the area.Information obtained has allowed us to understand the crust where the SRB and the Payenia volcanic province are located.Bouguer anomalies indicate that the SRB presents higher densities to the North of Cerro Nevado and Moho calculations suggest depths for this block between 40 and 50 km.Determinations of elastic thickness would indicate that the crust supporting the San Rafael Block presents values of approximately 10 km,being enough to support the block loading.However,in the Payenia region,elastic thickness values are close to zero due to the regional temperature increase.
