Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of st...Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.展开更多
Cartilage is a nonedible byproduct with little saleable value.However,previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential.The current study aim...Cartilage is a nonedible byproduct with little saleable value.However,previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential.The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon(Acipenser schrenckii)cartilage in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages.Five peptide sequences,including four novel peptides,were identified from ethanol-soluble cartilage hydrolysates.Among these five peptides,LTGP,LLLE,LLEL and VGPAGPAGP reduced the production of nitric oxide(NO)and interleukin-6(IL-6)while increasing interleukin-10(IL-10)excretion.Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase(MAPK)and interleukin-17(IL-17)signaling pathways after LLEL intervention.MAPK,which is involved in the IL-17 signaling pathway,was further proved to be blocked by downregulating the phosphorylation of p38,extracellular-signal regulated protein kinase(ERK),and c-jun N-terminal kinase(JNK).This novel peptide offers an attractive approach to develop functional foods.展开更多
To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The...To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The results showed that the Zn tolerance index of Aohan was significantly higher than other species.Among the four species,Aohan had the highest concentration of Zn in roots,followed by Golden Empress,Sanditi and Longxi.Aohan had the highest bioconcentration factor(BCF)in leaves.Whereas,Sanditi and Longxi had the lowest BCF in stem and roots,respectively.The translocation factor of Golden Empress was significantly lower than other species.The Zn accumulation rate of Aohan was higher than other species regardless of the concentration of Zn.Longxi had the lowest allocation of Zn in leaves and Golden Empress had the lowest allocation of Zn in roots.The concentrations of other elements(Fe and Mg)in leaves were decreased with Zn additions,but the interactions between Zn and other elements in roots varied with species.These results indicated that suitable species of alfalfa could successfully be used for the phytoremediation of Zn-contaminated soils.展开更多
In China, 31 genera and 95 species of fungivorous Phlaeothripinae are recorded here, of which 7 species are newly recorded and illustrated. An illustrated identification key to the 94 species is also provided, togethe...In China, 31 genera and 95 species of fungivorous Phlaeothripinae are recorded here, of which 7 species are newly recorded and illustrated. An illustrated identification key to the 94 species is also provided, together with the information of specimens examined, and distribution of each species.展开更多
文摘Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.
基金supported by the China Agriculture Research System(CARS-46),China.
文摘Cartilage is a nonedible byproduct with little saleable value.However,previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential.The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon(Acipenser schrenckii)cartilage in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages.Five peptide sequences,including four novel peptides,were identified from ethanol-soluble cartilage hydrolysates.Among these five peptides,LTGP,LLLE,LLEL and VGPAGPAGP reduced the production of nitric oxide(NO)and interleukin-6(IL-6)while increasing interleukin-10(IL-10)excretion.Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase(MAPK)and interleukin-17(IL-17)signaling pathways after LLEL intervention.MAPK,which is involved in the IL-17 signaling pathway,was further proved to be blocked by downregulating the phosphorylation of p38,extracellular-signal regulated protein kinase(ERK),and c-jun N-terminal kinase(JNK).This novel peptide offers an attractive approach to develop functional foods.
基金Supported by Natural Science Foundation of Shannxi Province,China(15JK1121,2015JM3086)the Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology,China(SLGPT2019KF04-02)+1 种基金Scientific research project of City-University co-construction of Shaanxi Province,China(SXJ-2101)The Project of Foreign Experts Bureau of Shaanxi Province of China(G2021041011L,2022WGZJ-20,G20200241015)。
文摘To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The results showed that the Zn tolerance index of Aohan was significantly higher than other species.Among the four species,Aohan had the highest concentration of Zn in roots,followed by Golden Empress,Sanditi and Longxi.Aohan had the highest bioconcentration factor(BCF)in leaves.Whereas,Sanditi and Longxi had the lowest BCF in stem and roots,respectively.The translocation factor of Golden Empress was significantly lower than other species.The Zn accumulation rate of Aohan was higher than other species regardless of the concentration of Zn.Longxi had the lowest allocation of Zn in leaves and Golden Empress had the lowest allocation of Zn in roots.The concentrations of other elements(Fe and Mg)in leaves were decreased with Zn additions,but the interactions between Zn and other elements in roots varied with species.These results indicated that suitable species of alfalfa could successfully be used for the phytoremediation of Zn-contaminated soils.
基金supported by the National Natural Science Fund for Distinguished Young Scientists(31025024)the National Natural Sciences Foundation of China(31272348)+1 种基金National Science Fund for Fostering Talents in Basic Research(J1210002)grants from the Ministry of Science and Technology of the People’s Republic of China(2014FY210200,2014FY110100,2013FY111200)
文摘In China, 31 genera and 95 species of fungivorous Phlaeothripinae are recorded here, of which 7 species are newly recorded and illustrated. An illustrated identification key to the 94 species is also provided, together with the information of specimens examined, and distribution of each species.
