Hybrid wheat is recognized as a preferred approach to improve wheat yield,and it will be a competition focus in high-tech seed industry in the future. We have made a breakthrough for the first time in creation of two-...Hybrid wheat is recognized as a preferred approach to improve wheat yield,and it will be a competition focus in high-tech seed industry in the future. We have made a breakthrough for the first time in creation of two-line hybrid wheat system,which reaches the world leading level in wheat research and has laid an important foundation for the future direction of the world wheat research. Similar to hybrid rice,the innovation of two-line hybrid wheat system is another achievement in science and technology. The application of hybrid wheat in China will greatly increase the food production,and make a great significance to food production and food security. This paper introduces the development process and major breakthrough of hybrid wheat in China,and the main bottleneck and countermeasures in the application of hybrid wheat.展开更多
Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair ...Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat（SSR） markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value（GSV） ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.展开更多
文摘Hybrid wheat is recognized as a preferred approach to improve wheat yield,and it will be a competition focus in high-tech seed industry in the future. We have made a breakthrough for the first time in creation of two-line hybrid wheat system,which reaches the world leading level in wheat research and has laid an important foundation for the future direction of the world wheat research. Similar to hybrid rice,the innovation of two-line hybrid wheat system is another achievement in science and technology. The application of hybrid wheat in China will greatly increase the food production,and make a great significance to food production and food security. This paper introduces the development process and major breakthrough of hybrid wheat in China,and the main bottleneck and countermeasures in the application of hybrid wheat.
基金funded by the Innovation Platform for Basic Research in Breeding of Beijing, China (D08070500690801)the Science and Technology Innovation Ability Construction Projects,Beijing Academy of Agricultural and Forestry Sciences, China (KJCX20140202)
文摘Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat（SSR） markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value（GSV） ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.
