OBJECTIVE:To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase(ROCK)/LIM-kinase1(LIMK1)/Cofilin signaling pathway.METHOD:Fifty Specific pathog...OBJECTIVE:To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase(ROCK)/LIM-kinase1(LIMK1)/Cofilin signaling pathway.METHOD:Fifty Specific pathogen Free Sprague-Dawley rats were randomly divided into five groups(n=8 each):blank group,model group,manipulation group,celecoxib group,and manipulation combined with celecoxib group(MC group).The osteoarthritis model was established by injecting 0.2 m L 4%papain into the articular disc of the rats.After successfully establishing the model,we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan(EX-LE4),Waixiyan(EX-LE5),Xuehai(SP10),Liangqiu(ST34),and Zusanli(ST36)acupoints for 10 min each time.Also,the celecoxib group was gavaged with 24 mg·kg^(-1)·d^(-1 )celecoxib,while the MC group was treated using both of these two methods.After four weeks,the cartilage of the right femur was removed for hematoxylin-eosin staining of the cartilage tissue.The expressions of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were observed using the enzyme-linked immunosorbent assay.Besides,we detected the expressions of ROCK,LIMK1,Phospho-LIM-kinase1(Phospho-LIMK1),Cofilin,and Phospho-Cofilin by Western blot.RESULTS:Compared to the model group,the manipulation group,celecoxib group,and MC group all exhibited superior results concerning pathological morphologic changes of cartilage,as observed by hematoxylin-eosin staining and calculated using the Mankin score.Besides,in contrast to the blank group,the model group exhibited elevated serum levels of IL-1βand TNF-α(P<0.01),while the expression of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage were all higher(P<0.01).Also,the serum levels of IL-1βand TNF-αin each treatment group were lower(P<0.01)than in the model group.Moreover,there were lower expressions of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage in the manipulation group and the MC group(P<0.01).Compared with the model group,the expression of ROCK,LIMK1,PhosphoLIMK1,Cofilin,and Phospho-Cofilin in cartilage in the celecoxib group were not statistically different(P>0.05).CONCLUSION:In this study,we established that manipulation has a better curative effect than celecoxib.Manipulation inhibits the development of cytoskeleton damage in cartilage and slows articular degeneration by regulating the expression of related proteins in the cytoskeletal signaling pathway.展开更多
Objective:To observe the differences in clinical therapeutic effect on acute attack of lumbar disc herniation(LDH) treated with electroacupuncture(EA) of different wave patterns so as to optimize EA wave pattern in th...Objective:To observe the differences in clinical therapeutic effect on acute attack of lumbar disc herniation(LDH) treated with electroacupuncture(EA) of different wave patterns so as to optimize EA wave pattern in the treatment of acute attack of LDH.Methods:A total of 108 patients were randomized into three groups,i.e.,a disperse-dense wave,an intermittent wave and a continuous wave group,36 cases in each one.In all of three groups,Shangliao(上髎BL31),Ciliao(次髎BL32),Zhongliao(中髎BL33) and Xialiao(下髎BL34) were selected bilaterally as the main acupoints,which is generally termed as Baliao(八髎BL31 to BL34,bilateral).In each group,the corresponding wave pattern was used in EA.The treatment lasted 20 min each time,once daily,totally for 10 days.Before and after treatment,the score of visual analogue scale(VAS) and the score of the lower back pain of Japanese orthopedic association(JOA) were observed in the patients of three groups and the overall therapeutic effect was evaluated.Results:After treatment,the VAS score was decreased and JOA score was increased in the patients of 3 groups,indicating statistical significance(all P <0.05).In comparison of VAS score before and after treatment among three groups,the score in the disperse-dense wave group was higher than the intermittent wave group and the continuous wave group,indicating statistical differences(both P <0.05).In comparison of JOA score before and after treatment among three groups,the score in the continuous wave group was higher than the intermittent wave group and the disperse-dense wave group,indicating statistical differences(both P <0.05).The total effective rate was 94.12% in the disperse-dense wave group,94.29% in the intermittent wave group and 97.14% in continuous wave group,without significant difference among groups(P> 0.05).Conclusion:Electroacupuncture of 3 different wave patterns all effectively relieves clinical symptoms of acute attack of lumbar disc herniation.Regarding pain release,electroacupuncture with disperse-dense wave is better than intermittent wave and continuous wave.Regarding the improvement of lumbar function,the effect of electroacupuncture with continuous wave is better than disperse-dense wave and intermittent wave.展开更多
基金Supported by the National Natural Science Foundation of China(No.81273870)Chongqing Municipal Health and Family Planning Commission and Chongqing Municipal Science and Technology Commission Jointly Funded Key Research Projects in Traditional Chinese Medicine(No.ZY201801007)Beibei District Chongqing Basic Research and Frontier Exploration Project(No.2019-6)。
文摘OBJECTIVE:To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase(ROCK)/LIM-kinase1(LIMK1)/Cofilin signaling pathway.METHOD:Fifty Specific pathogen Free Sprague-Dawley rats were randomly divided into five groups(n=8 each):blank group,model group,manipulation group,celecoxib group,and manipulation combined with celecoxib group(MC group).The osteoarthritis model was established by injecting 0.2 m L 4%papain into the articular disc of the rats.After successfully establishing the model,we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan(EX-LE4),Waixiyan(EX-LE5),Xuehai(SP10),Liangqiu(ST34),and Zusanli(ST36)acupoints for 10 min each time.Also,the celecoxib group was gavaged with 24 mg·kg^(-1)·d^(-1 )celecoxib,while the MC group was treated using both of these two methods.After four weeks,the cartilage of the right femur was removed for hematoxylin-eosin staining of the cartilage tissue.The expressions of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were observed using the enzyme-linked immunosorbent assay.Besides,we detected the expressions of ROCK,LIMK1,Phospho-LIM-kinase1(Phospho-LIMK1),Cofilin,and Phospho-Cofilin by Western blot.RESULTS:Compared to the model group,the manipulation group,celecoxib group,and MC group all exhibited superior results concerning pathological morphologic changes of cartilage,as observed by hematoxylin-eosin staining and calculated using the Mankin score.Besides,in contrast to the blank group,the model group exhibited elevated serum levels of IL-1βand TNF-α(P<0.01),while the expression of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage were all higher(P<0.01).Also,the serum levels of IL-1βand TNF-αin each treatment group were lower(P<0.01)than in the model group.Moreover,there were lower expressions of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage in the manipulation group and the MC group(P<0.01).Compared with the model group,the expression of ROCK,LIMK1,PhosphoLIMK1,Cofilin,and Phospho-Cofilin in cartilage in the celecoxib group were not statistically different(P>0.05).CONCLUSION:In this study,we established that manipulation has a better curative effect than celecoxib.Manipulation inhibits the development of cytoskeleton damage in cartilage and slows articular degeneration by regulating the expression of related proteins in the cytoskeletal signaling pathway.
文摘Objective:To observe the differences in clinical therapeutic effect on acute attack of lumbar disc herniation(LDH) treated with electroacupuncture(EA) of different wave patterns so as to optimize EA wave pattern in the treatment of acute attack of LDH.Methods:A total of 108 patients were randomized into three groups,i.e.,a disperse-dense wave,an intermittent wave and a continuous wave group,36 cases in each one.In all of three groups,Shangliao(上髎BL31),Ciliao(次髎BL32),Zhongliao(中髎BL33) and Xialiao(下髎BL34) were selected bilaterally as the main acupoints,which is generally termed as Baliao(八髎BL31 to BL34,bilateral).In each group,the corresponding wave pattern was used in EA.The treatment lasted 20 min each time,once daily,totally for 10 days.Before and after treatment,the score of visual analogue scale(VAS) and the score of the lower back pain of Japanese orthopedic association(JOA) were observed in the patients of three groups and the overall therapeutic effect was evaluated.Results:After treatment,the VAS score was decreased and JOA score was increased in the patients of 3 groups,indicating statistical significance(all P <0.05).In comparison of VAS score before and after treatment among three groups,the score in the disperse-dense wave group was higher than the intermittent wave group and the continuous wave group,indicating statistical differences(both P <0.05).In comparison of JOA score before and after treatment among three groups,the score in the continuous wave group was higher than the intermittent wave group and the disperse-dense wave group,indicating statistical differences(both P <0.05).The total effective rate was 94.12% in the disperse-dense wave group,94.29% in the intermittent wave group and 97.14% in continuous wave group,without significant difference among groups(P> 0.05).Conclusion:Electroacupuncture of 3 different wave patterns all effectively relieves clinical symptoms of acute attack of lumbar disc herniation.Regarding pain release,electroacupuncture with disperse-dense wave is better than intermittent wave and continuous wave.Regarding the improvement of lumbar function,the effect of electroacupuncture with continuous wave is better than disperse-dense wave and intermittent wave.
